Search PubMed⌕ Search

Biomedical subjects

M Goodman

Publications and source records attributed to M Goodman.

At least 433 records · Page 24Linked to original sources
PubMed1978

Long-term follow-up and bronchial reactivity testing in survivors of the adult respiratory distress syndrome.

Long-term follow-up of 9 survivors of the adult respiratory distress syndrome at 39 to 83 months demonstrated 2 patients with continued improvement of their restrictive pattern, 3 patients with stable mild obstructive disease, one patient who had had chronic obstructive pulmonary disease before the adult respiratory distress syndrome with continued pulmonary deterioration, and 4 patients without symptoms and with normal pulmonary function tests. Attention is drawn to bronchial reactivity as defined by a bronchodilator response of more than 60 per cent increase in maximal mid-expiratory flow, positive methacholine challenge, or development of bronchospasm after exercise in 3 patients having clinical symptoms of wheezing on recovery.

Adolescent↗
PubMed1978

Cell movement and the mechanism of invasiveness: a survey of the behaviour of some normal and malignant cells implanted into the developing chick wing bud.

A survey of the behaviour of a variety of normal and malignant tumours and cells has been carried out to gain insights into the mechanisms of tumour invasiveness. The tumours and cells were implanted into the developing chick wing bud, which is a loose mesenchyme bounded by ectoderm. The distribution of the grafted cells was examined histologically after one or two days. The special feature of this assay is that the behaviour of cells is tested in a 3-dimensional tissue. Cells from 3 different carcinomas, mouse lung tumour, rat bladder tumour and human breast tumour did not invade the mesenchyme, whereas trophoblast, sarcoma 180, cultured hamster fibroblasts (BHK, PyBHK, Nil 8, HSV Nil 8) and neuroblastoma cells did. Cells from embryonic pigmented retina and heart ventricle were non-invasive. These results suggest that cell movement may not be a common feature of all invasive tumours. The cells that did move into the mesenchyme appeared to do so by various mechanisms. Lack of contact inhibition of movement, although probably involved in the invasiveness of sarcoma 180 cells, does not appear to be necessary for invasion: cells that have been shown to exhibit contact inhibition of movement (BHK and PyBHK) also invade. Both normal and transformed cells (BHK and PyBHK; Nil 8 and HSV Nil 8) moved into the mesenchyme. Other invading cells, such as trophoblast, neuroblastoma and to a small extent, HSV Nil 8 cells, destroy the adjacent host tissue and this may be important in the invasiveness of these cells. The patterns of invasion and interactions with the host tissue were varied. Trophoblast and the fibroblasts were often elongated along the basement membrane at the ectoderm/mesenchyme border and also closely apposed to the endothelial linings of blood vessels. Sarcoma 180 and neuroblastoma cells clustered around nerves. The embryonic tissues and neuroblastoma cells were often associated with blood vessels. These results are discussed in relation to tumour invasion. A striking finding was that the carcinoma cells were frequently found positioned within the wing ectoderm on the basement membrane. This affinity of carcinoma cells for the epithelium rather than the mesenchyme leads to a reappraisal of the mechanisms involved in the invasiveness of carcinomas.

Animals↗
PubMed1978

Mechanisms of invasiveness of epithelial tumours: ultrastructure of the interactions of carcinoma cells with embryonic mesenchyme and epithelium.

Three different kinds of carcinoma cell, human breast tumours, mouse lung tumour and rat bladder tumours have been implanted into the developing chick wing to assay their invasive behaviour. We found that the majority of the carcinoma cells did not invade the mesenchyme but were positioned in the ectoderm. We examined the ultrasturcture of the interaction of the carcinoma cells with both the ectoderm and the mesenchyme to see if there are any differences. We found no specialized adhesive junctions between carcinoma cells and mesenchyme cells. In contrast, the carcinoma cells seem to be adhesive to the ectoderm cells; desmosomes between the two have been found and also frequent parallel alignment of membranes over long distances. This difference between the interaction of the carcinoma cells with the mesenchyme and the ectoderm may explain why the carcinoma cells can penetrate into the ectoderm but rarely do so into the mesenchyme. The carcinoma cells formed a smooth border with the mesenchyme even in the absence of a basal lamina. These results are discussed in relation to the mechanisms of invasion of epithelial tumours in vivo. In particular, the relative importance of the basal lamina, and cell adhesion within the tumour, in limiting invasiveness are assessed.

Animals↗
PubMed1978

Cell contacts and sorting out in vivo: the behaviour of some embryonic tissues implanted into the developing chick wing.

The interaction of cells from embryonic liver, neural retina and mesonephros with cells from limb-bud mesenchyme has been investigated in vivo by grafting these tissues into the developing chick wing-bud. The implanted cells were in all cases from quail tissue which can be recognized histologically. As embryonic liver and neural tube are tissues that sort externally to limb-bud mesenchyme in mixed aggregates, it would be expected, from a differential adhesiveness hypothesis, that heterotypic adhesions along the borders of graft and host would be favoured over cell-cell adhesions in the graft. No morphological signs of this were evident: rather the grafted cells maximized like-like contacts. The cells of the grafts, including those from control mesenchyme, did not invade into the wing. The results were the same irrespective of whether the graft was a fragment of tissue or a pellet of reaggregated cells. This supports the idea that cells within tissues are not actively moving around and also provides controls for assaying the invasiveness of other cell types, such as malignant cells into the wing.

Animals↗
PubMed1977

Primitive haemoglobin.

The sequences of Petromyzon and Aplysia globins are compared with the postulated vertebrate and mollusc-vertebrate ancestors to see if differences exist in the rates of evolution of different types of residue positions. Between the mollusc-vertebrate ancestor and Aplysia globin there is no very striking pattern of changes except that the interior positions are relatively conserved. In the evolution of Petromyzon haemoglobin, the alpha 1 beta 2 contact area is relatively conserved. The homopolymeric binding of lamprey Hb seems to be a primitive function.

Animals↗
PubMed1977

Alignment statistic for identifying related protein sequences.

Closely related proteins show an obvious kinship by having numerous matching amino acids in their aligned sequences. Kinship between anciently separated proteins requires a statistical evaluation to rule out fortuitous similarities. A simple statistic is developed which assumes equal probability for all codon pairs, and a table of critical values for amino acid sequence alignments of lengthnments of length 200 or less is presented. Applying this statistic to V and C regions of immunoglobulin chains, aligned on the basis of shared features of three-dimensional structure, provides evidence that the V and C sequences descended from a common ancestor. Similarly the distant evolutionary relationship of dehydrogenases, flavdoxin, and subtilisin, suggested by structural alignments, is verified. On the other hand, the statistic does not verify a common evolutionary origin for the heme binding pocket in globins and cytochrome bs. Empirical evidence from the distribution of MMD values of amino acid pairs in comparisons of misaligned polypeptide chains and from Monte Carlo trials of sequences aligned with arbitrary gaps supports the validity of the statistic.

Amino Acid Sequence↗
PubMed1977

Traumatic neuroma of the nose.

We report a case of a traumatic neuroma of the nose. It was an unusual site for two distinct types of tissue response to skin and soft trauma that exceeded the reparative needs of the body. In the first instance, a small cutaneous nerve was sectioned, and its inability to establish continuity distally resulted in a neuroma. In the second instance, the excision site of the neuroma was reinjured, and an abnormal connective tissue response occurred, resulting in a hypertrophic scar or keloid. Both were treated similarly with excision, and ultimately with local steroid injection with satisfactory results.

Adolescent↗
PubMed1977

Management of malignancy of the temporal bone.

An analysis was made of 31 patients with malignancy of the temporal bone treated at Massachusetts Eye and Ear Infirmary between 1959 and 1975. Treatment modalities included: partial temporal bone resection, subtotal temporal bone resection, total temporal bone resection, radical mastoidectomy followed by radiation therapy, radiation therapy alone, and chemotherapy. When tumor was limited to the external ear canal, partial temporal bone resection was effective in 10 out of 11 patients. When tumor involved the middle ear, subtotal temporal bone resection cured 3 out of 5 patients, and radical mastoidectomy followed by radiation therapy cured 5 out of 12 patients. Total temporal bone resection and chemotherapy failed to cure a patient each, but radiation therapy alone was successful in controlling tumor in one patient.

Adult↗
PubMed1977

Amino acid sequences of the aplpha and beta chains of adult hemoglobin of the tupai, Tupaia glis.

Globin prepared from hemoglobin of adult tupai (Tupaia glis) was separated into alpha and beta polypeptide chains by CM-cellulose column chromatography. The S-aminoethylated alpha polypeptide chain and S-carboxymethylated beta polypeptide chain were each digested with trypsin, and the sequences of all the peptides thus obtained were established. The ordering of these tryptic peptides in the alpha and beta polypeptide chains was deduced from the homology of their primary structures with that of human adult hemoglobin. In this way the primary structures of the alpha and beta polypeptide chains of tupai hemoglobin were established; 27 amino acids in the alpha polypeptide chain and 26 in the beta chain differ from those in human adult hemoglobin.

Amino Acid Sequence↗