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Biomedical subjects

M Gomes

Publications and source records attributed to M Gomes.

61 records · Page 4Linked to original sources
PubMedDate not supplied

Immunologic methods for the detection of benzo[a]pyrene metabolites in urine.

Two monoclonal antibodies (10C10 and 4D5) have been developed from the spleen cells of Balb/c mice immunized with 6-aminobenzo[a]pyrene covalently coupled to bovine serum albumin. These antibodies have been used in an immunoassay for the detection of benzo[a]pyrene and its metabolites in mouse urine. The antibodies were characterized in terms of sensitivity and specificity by competitive enzyme-linked immunosorbent assay (ELISA). With both antibodies, 50% inhibition of antibody binding is at 4 pmol of BP. The antibodies also cross-react with a number of BP metabolites as well as with several other polycyclic aromatic hydrocarbons (PAHs) including pyrene, 1-aminopyrene, and 7,12-dimethylbenz[a]anthracene but with different sensitivities. These results suggest that this assay will detect multiple PAH metabolites in urine. To test the assay on biological samples, mice were treated with [3H]BP, and urine was collected and digested with beta-glucuronidase and aryl sulfatase. Several methods were used to isolate BP and its metabolites from the urine, including ethyl acetate extraction, Sep-pak C18 cartridge chromatography, XAD2 resin chromatography, and immunoaffinity chromatography with antibody 4D5. Analysis of the urine extracts with antibody 4D5 gave 50% inhibition at 12-15 pmol of metabolites. Thus, quantitation of metabolites in this sample by competitive ELISA against a standard curve of BP would have underestimated actual metabolite levels by about 70%. This assay will be applied to the analysis of urines from individuals with environmental or occupational exposure. Since humans are usually exposed to BP in complex mixtures of PAHs, multiple metabolites may be present in the urine, making absolute quantitation difficult. This assay should thus serve as a general indicator of exposure to this class of chemicals.

Animals↗
PubMedDate not supplied

[Surgical procedures in selected proctological patients with local anesthesia. Study of 150 cases].

Experience in the treatment of 150 patients with anorectal disorders and disorders of the sacrococcygeal region who were operated on with local anesthesia at the University Hospital, ABC Medical School, São Bernardo do Campo, SP, Brazil, from March 1995 to March 1998. The anesthesia technique, the operations carried out and the tolerance to the procedure are reported. Intraoperative morbidity was 10.6% (16 patients), and postoperative morbidity was 6% (nine patients). The age of patients was between 15 and 92 years old, with mean age 42 years old; 58% of patients were male and 42% female. Surgical mean time was 45 minutes and the patients remained in the hospital for a mean time of 8 hours. All of patients was instructed about the anesthesia technique, their advantages and disadvantages, and only with their permit the surgery was programmed. Hospitalization was required in five patients (3.3%). The anesthesia technique employed was the same for all patients. Upon survey, 96.7% of the patients stated they did not feel pain during the surgery and that they would go through the procedure again. The authors conclude the surgical treatment of anorectal disorders and disorders of the sacrococcygeal region with local anesthesia is viable and safe, and in addition, is well accepted by the patients.

Adolescent↗
PubMedDate not supplied

Laser in situ keratomileusis for myopia using manual dissection.

Forty-two eyes underwent interlamellar photoablation, a simple method of exposing the stroma to excimer laser treatment using common surgical instruments. A 8 mm diameter line up to 200 microns thick is raised manually from its bed. The stromal bed is reshaped with the excimer laser, and restoring the disc results in correction of high myopia. Our own nomogram was used. This method allows the use of less corticosteroids and final visual acuity recovers between 20 and 90 days postoperatively, time required for interface adherence and epithelial remodeling. Two treatment zones were used when 80% of the patient's myopia was greater than 10.00 diopters (D). One-year follow-up data allowed us to conclude that this method achieved results similar to excimer laser in situ keratomileusis (LASIK).

Corneal Stroma↗
PubMedDate not supplied

Keratomileusis-in-situ using manual dissection of corneal flap for high myopia.

Overcorrection, regression and haze are some side-effects found after excimer laser photorefractive keratectomy (PRK) for high myopia. A new method attempts to avoid photoablation through Bowman's layer, using the stroma to flatten the cornea without use of a microkeratome. Manual surgical instruments such as the diamond blade, spatula, Pierce forceps, and Vannas scissors are used to remove a disc of anterior cornea. Minimal topical corticosteroids are used, avoiding the complications of prolonged corticotherapy. Six eyes underwent manual excimer laser keratomileusis-in-situ. Postoperatively, the epitheliums in these eyes initially were dry and excoriated. By the twentieth day, however, the eyes had re-epithelialized and recovered. The optical effect is the same as when keratomileusis is used. No more than three-fourths of the pre-existing myopia was used in the program as some undercorrection was desired.

Adult↗