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M Ghazizadeh

Publications and source records attributed to M Ghazizadeh.

52 records · Page 3Linked to original sources
PubMed1985

Immunohistological detection of T antigen and ABH blood group antigens in upper urinary tract tumours.

The status of T antigen was assessed by means of peanut agglutinin (PNA) used in an immunoperoxidase technique, and the status of ABH blood group antigens was determined by the specific red cell adherence (SRCA) test. Thirty-eight patients with upper urinary tract tumours were examined. Our data indicated that T antigen status did not correlate with conventional prognostic indicators such as grade and stage of tumour and survival of patients, while the expression or deletion of ABH blood group antigens did correlate well. It was concluded that ABH blood group antigen determination might provide a useful prognostic probe in upper urinary tract tumours should it find clinical application.

ABO Blood-Group System↗
PubMed1984

Immunohistochemical localization of T antigen-like substance in benign hyperplasia and adenocarcinoma of the prostate.

Peanut agglutinin was used in a lectin-anti-lectin immunoperoxidase technique to assess the status of T antigen-like substance in histological sections of benign hyperplasia and adenocarcinoma of the prostate. Of 30 benign lesions 26 (86.7 per cent) had negative staining in the glandular epithelia and 4 showed uneven staining of the epithelial cells in a small proportion of the glands. Additionally, 15 of 30 benign lesions (50 per cent) had positive staining restricted to the basal myoepithelial cells in a variable proportion of the glands. All benign lesions demonstrated the presence of normal cryptic T antigen after neuraminidase digestion. Of 25 adenocarcinomas 14 (56 per cent) were positive and 7 (25 per cent) were negative for T antigen but demonstrated the presence of normal cryptic T antigen after neuraminidase digestion, and 4 (16 per cent) were negative for T and cryptic T antigens. Of 8 grade 1, 9 grade 2 and 8 grade 3 tumors, and 9 tumors from patients with bone metastases 3 (37.5 per cent), 8 (88.9 per cent), 7 (87.5 per cent) and 7 (77.8 per cent), respectively, were positive for T antigen or negative for T and cryptic T antigens. The results show a correlation between the status of T antigen-like substance and tumor grade as well as metastasis in patients with prostatic carcinoma.

Adenocarcinoma↗
PubMed1984

Immunohistochemical detection of carcinoembryonic antigen in benign hyperplasia and adenocarcinoma of the prostate with monoclonal antibody.

Immunoperoxidase localization of carcinoembryonic antigen was done on tissue sections of benign hyperplasia and adenocarcinoma of the prostate using murine monoclonal antibody against carcinoembryonic antigen. The study was done to determine whether carcinoembryonic antigen would be a tissue marker of prostatic malignancy. Of 30 benign lesions 25 (83 per cent) were negative and 5 had focally weak staining, while of 36 adenocarcinomas 31 (86 per cent) had diffusely positive staining and 5 were negative. Diffusely moderate or strong staining was noted in 5 of 10 grade I (50 per cent), 11 of 12 (92 per cent) grade II and 13 of 14 (93 per cent) grade III tumors, as well as 14 of 15 (93 per cent) tumors from patients with bone metastasis. These findings suggested that extensive immunostaining for carcinoembryonic antigen in prostatic glandular lesions might be a potentially important prognostic marker of malignancy. Further studies are warranted to extend and confirm these results.

Adenocarcinoma↗
PubMed1984

Prostatic origin of metastases: immunoperoxidase localization of prostate-specific antigen.

An immunoperoxidase technique for prostate-specific antigen was used to evaluate autopsy material from 8 patients with multiple prostatic metastases, 22 primary prostatic carcinomas and 12 nonprostatic tumors. All specimens of primary and metastatic prostatic carcinomas stained positively, irrespective of the Gleason grade. None of the nonprostatic tumors showed immunostaining. Thus, the method appeared to be highly specific and highly sensitive for the detection of the prostatic origin of metastases.

Antigens, Neoplasm↗
PubMed1983

A, B, O(H) blood group antigen distribution in normal skin and squamous cell carcinoma of the penis.

The distribution of A, B, O(H) blood group antigens was studied in paraffin sections of 4 and 11 specimens, respectively, of normal skin and squamous cell carcinoma of the penis using specific red cell adherence (SRCA) test. In the normal skin of the penis the antigens appeared on the uppermost cornifying layers, while basal and malpighian layers were devoid of antigens. In squamous cell carcinoma of the penis the antigens were either absent or confined only to the epithelial pearl formations. Each of the 4 normal skins and 3 of the 11 carcinomas were antigen positive. As the blood group antigens were frequently absent in patients with carcinoma of the penis regardless of the tumor grade and stage as well as patient's condition, they lack the power to discriminate invasive potential.

ABO Blood-Group System↗
PubMed1983

Clinical features of urachal carcinoma in Japan: review of 157 patients.

Carcinoma of the urachus is not as uncommon as previously considered. 157 cases diagnosed to have urachal carcinoma collected from the Japanese literature were reviewed and the findings were compared with those reported in the English literature. The incidence of the disease ranges from 0.55 to 1.2% of bladder tumours in Japan in contrast with 0.07 to 0.70% in the Western countries. Males accounted for 72% of the patients. The highest age incidence occurred between fifth and sixth decades. The commonest presenting symptom was haematuria (71%). Adenocarcinoma accounted for 88% of the tumours most being mucous producing. Various treatment modalities were used, however, and the prognosis remained uniformly poor. An analysis of 66 patients with known outcome revealed an overall 5-year survival rate of 6%. The authors conclude that the disease tends to have a relatively higher incidence in Japan (Far East). When comparing the parameters of sex, age, presenting symptom, histopathology, treatment and prognosis, urachal carcinoma appears to have the same characteristics in the Far East as in Western countries.

Adenocarcinoma↗
PubMed1983

Direct immunofluorescence for ABH blood group isoantigens: use of FITC-conjugated lectins.

A direct immunofluorescence method was used to detect A, B, or H blood group isoantigens in deparaffinized tissue sections of 30 bladder tumors of various grades and stages. The results were compared with those of the specific red cell adherence test performed on the replicate sections. The two methods showed identical results for isoantigens A, B, and H. Direct immunofluoresence method appeared to be less time-consuming, more simple to perform, and reproducible with less subjectivity in the interpretation of the results.

ABO Blood-Group System↗
PubMed1983

Specific red cell adherence test in benign and malignant lesions of the prostate.

The specific red cell adherence test (SRCA) for blood group antigens has been shown to have some bearing on the invasive potential of bladder tumours. Hitherto there have been few data published from patients with prostatic disease. The results of SRCA testing in 69 such patients are presented. Each of the 30 cases of adenocarcinoma was antigen negative. However, as 18 of 39 patients with only benign hyperplasia were also antigen negative, the test clearly does not reflect extant tumour and is probably not an indicator of subsequent growth of prostatic cancer. Antigen expression was also negative in sections showing prostatitis. As the test was invariably negative in patients with adenocarcinoma, whether or not metastases were present and whatever the degree of differentiation of the primary tumour, it lacks the power to discriminate invasive potential.

ABO Blood-Group System↗
PubMed1983

Prognostic validity of the specific red cell adherence test in upper urothelial tumours.

The specific red cell adherence (SRCA) test for blood group antigens was used in 32 patients with upper urinary tract tumours to determine whether their survival could be predicted by this test. The SRCA results correlated well with survival in each grade and stage of the tumours. Overall, patients with positive tests showed a significantly higher 5-year survival rate than those with negative tests (87.2% vs. 24.6%). These findings add support to the prognostic importance of this test in upper urinary tract tumours.

Adult↗
PubMed1983

In vitro-produced antibody-coated bacteria.

A new in vitro method was developed which enabled isolation and identification of ACB-positive bacteria in polymicrobial renal bacteriuria. This approach was used in 16 patients with permanent nephrostomies and asymptomatic bacteriuria who had shown positive ACB test by the method of Thomas et al. A total of 48 urinary isolates were obtained from these patients in which 21 (43.8%) were revealed as being positive by the present method. In 13 of the 16 patients, there was only one positive species among several species of bacteria. The species with a colony count of more than 10(5)/ml was found to be most commonly ACB positive (71.5%). Of the 13 patients who had creatinine clearance tests available, 11 (84.6%) showed a considerable decrease in renal function after the intervals since nephrostomy. The immunologically different characteristics observed among multiple bacterial species in polymicrobial renal bacteriuria suggest a possible aggressive role for the ACB-positive species and therefore a need for eradicating them.

Aged↗
PubMed1982

Specific red cell adherence testing of benign and malignant lesions of the urethra.

We herein report the first application of the specific red cell adherence test in patients with benign and malignant lesions of the urethra. In an attempt to determine whether the specific red cell adherence test could have potential use in predicting malignant behavior of urethral carcinoma we applied this test on specimens from 17 patients with a urethral caruncle, 3 with chronic urethritis and 4 with primary urethral carcinoma. Of the 20 benign lesions 18 (90 per cent) were positive for adherence, while the 4 urethral carcinoma specimens were negative. The high positive rate obtained in specimens of benign urethral lesions, together with the uniformly negative tests in those of urethral carcinoma, indicate the possible use of the specific red cell adherence test in early detection of aggressive behavior of urethral carcinoma, as well as predictiveness in bladder cancer.

ABO Blood-Group System↗
PubMedDate not supplied

Immunohistochemical expression of T, Tn and sialyl-Tn antigens and clinical outcome in human breast carcinoma.

BACKGROUND: The extent of expression of reactive T (Thomsen-Friedenreich), Tn and sialyl-Tn antigens has been assumed to predict carcinoma aggressiveness. We studied the expression of T, Tn and sialyl-Tn antigens in a relatively large cohort of breast carcinoma patients with known long-term outcome to assess the clinical and biological significance of these antigens. MATERIALS AND METHODS: T, Tn and sjalyl-Tn antigens were examined in 72 consecutive primary breast carcinomas by immunohistochemistry using well defined monoclonal antibodies and their semiquantitative values were correlated with established clinicopathologic prognostic parameters of the disease to determine their relationship with long-term clinical outcome. RESULTS: Of the 72 carcinomas, 63 (87.5%) each expressed T or Tn antigens, while 16 (22%) expressed sialyl-Tn antigens. Most carcinomas (81%) expressed more than one of the antigens simultaneously, being the most frequent combination T/Tn antigen expression. No significant correlation was noted between the expression of T, Tn and sialyl-Tn antigens (whether individually or in combination) and the prognostic parameters including patient age, disease stage, tumor size, lymph node status, nuclear and histologic grades, histologic types, hormone receptor status and menopausal status. Univariate survival analyses showed that disease stage, tumour size and lymph node metastasis were significant predictors of overall survival. Interestingly, a significant inverse correlation was found between the Tn antigen expression (p = 0.04), as well as the combined T/Tn (p = 0.03) and Tn/sialyl-Tn (p = 0.02) antigen expressions and long-term overall survival. In a multivariate Cox proportional hazard model, disease stage and a negative or low Tn antigen expression emerged as significant predictors of overall survival. CONCLUSION: Our data suggested that the expression of T, Tn and sialyl-Tn antigens does not appear to predict the outcome of patients with breast carcinoma in a long-term run. Moreover, the findings signified a potential value for a negative or low Tn antigen expression in prognostic stratification of breast carcinomas.

Adult↗
PubMedDate not supplied

A new antigen retrieval method using citraconic anhydride for immunoelectron microscopy: localization of surfactant pro-protein C (proSP-C) in the type II alveolar epithelial cells.

In the attempt to develop a pre-embedding immunoelectron microscopic method with improved ultrastructral morphology, we examined an antigen retrieval (AR) method using citraconic anhydride, and compared the effects of glutaraldehyde fixation with routine paraformaldehyde fixation in the pre-embedding immunoelectron microscopic method with reference to the localization of surfactant-associated pro-protein C (proSP-C) in the lung. The glutaraldehyde-fixed tissues were immunostained after AR in 0.05% citraconic anhydride solution, pH 7.4, at 98 degrees C for 60 min. In routine pre-embedding immunoelectron microscopic method using paraformaldehyde fixation, proSP-C positive products were distributed sporadically in the type II alveolar epithelial cells. In glutaraldehyde-fixed tissues without the AR method, proSP-C products were not detected, however after AR in citraconic anhydride proSP-C positive products were distributed specifically, in rough endoplasmic reticulum membranes, Golgi complex membranes, multivesicular bodies and osmiophilic lamellar bodies. The positive proSP-C products also showed lattice-like structures in the alveoli. Thus, the present AR method provides successful pre-embedding immunoelectron microscopy of glutaraldehyde-fixed tissues.

Animals↗