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Biomedical subjects

M Gabriele

Publications and source records attributed to M Gabriele.

At least 37 records · Page 2Linked to original sources
PubMed1990

Induction of micronuclei by mitomycin C and colchicine in the marine mussel Mytilus galloprovincialis.

The frequencies of micronuclei (MNi) in the gill cells of the mussel Mytilus galloprovincialis were determined over a long period (up to 28 days) following a 48-h treatment with colchicine. The frequency of MNi at the end of treatment was significantly higher than in controls and 24 h later it had increased even more. After this period, the frequency of MNi rapidly declined until a plateau level was reached on day 2-3, which was significantly higher than the control baseline level, and persisted until the end of the experiment (28th day). In the same cell system we previously reported a persistence of an increased frequency of MNi after treatment with mitomycin C (MMC) (Majone et al., 1987). In order to establish the origin of MNi, the difference between their size distribution in MMC- and colchicine-treated animals was determined at the end of treatment as well as during the plateau phase. The difference was statistically significant (P less than 0.001) at the end of treatment, the MNi induced by MMC being smaller than those induced by colchicine. However, the difference during the plateau phase was not statistically significant. Human CREST antikinetochore fluorescent antibodies reacted with chromosome centromeres of Mytilus and were applied to gill cells at the end of a 48-h treatment with MMC or colchicine. About 60% of the MNi induced by colchicine but only 30% of those produced by MMC reacted positively with the fluorescent antibodies. This result indicates that the majority of MNi observed at the end of a 48-h treatment with MMC or colchicine originate, respectively, from acentric chromosome fragments and from whole lagging chromosomes.

Animals↗
PubMed1990

Roxithromycin penetration into gingiva and alveolar bone of odontoiatric patients.

The concentrations of the new macrolide antibiotic roxithromycin in plasma, saliva, gingiva, and alveolar bone were studied in 24 odontoiatric patients treated with a first dose of 300 mg p.o. followed by three maintenance doses of 150 mg p.o., 12-hourly. Samples of blood, saliva, gingiva, and bone were collected at various time points up to 24 h after the last dosing, and the roxithromycin concentration was measured microbiologically, using Bacillus subtilis ATCC 6633 as the reference organism. Pharmacokinetic analysis was performed according to a two-compartment open model with first-order absorption. The plasma, gingiva, and alveolar bone peak concentrations were 6.12 +/- 1.94 mg/l, 6.55 +/- 2.54 mg/kg, and 5.09 +/- 1.60 mg/kg, respectively. Low levels of roxithromycin were detected in saliva (0.67 +/- 0.12 mg/l at the 3rd h). The values of the area under the concentration-time curve for plasma, gingiva, and bone were 59.47 mg/l.h, 51.88 mg/kg.h and 46.80 mg/kg.h, respectively; the half-life values were 7.52 h for plasma and 6.36 and 5.20 h for gingiva and bone, respectively. These results indicate that roxithromycin reaches high levels in periodontal tissues.

Adult↗
PubMed1989

[Value of cell cycle analysis by flow cytometry in gynecology and obstetrics].

Flow cytometry is a new technique that makes it possible to study the cell cycle and the DNA content of thousands of cells per minute. Its value in oncology is prognostic, there being a correlation between the level of DNA and mitotic activity with the power to invade tissues. After a description of the cell cycle, a review of the literature demonstrates its value in gynaecology and obstetrics. Aneuploidy and marked mitotic activity correlate well with a poor prognosis for: CIN: aneuploid lesions are less likely to regress spontaneously and are found more frequently in higher grades. This is a reason for treating aneuploid CIN. Adenocarcinoma of the endometrium: aneuploidy is the best prognostic measure when analysing cells in a multifactorial way to include the stage, the grade, the degree of myometrial invasion and the receptors. Sarcoma of the uterus: in a small series aneuploidy occurred frequently and was of poor prognosis. Molar pregnancy: flow cytometry is a diagnostic aid because the hydatidiform mole is diploid with a marked degree of mitotic activity. The prognostic value has still to be determined. Ovary: borderline tumours are always diploid. In cancers of the ovary aneuploidy is a sign of aggression and predictive of survival.

Aneuploidy↗
PubMed1988

Exposure to agricultural treatment residues: some simple statistical considerations based on monitoring data for ethoxyquin on apples.

The data examined here concern ethoxyquin levels, mainly measured in apple samples from the retail market. In this study about three hundred measurements, carried out on apples in Northern-Central Italy and recently published, are examined, with the purpose of defining the main characteristics of value statistical distribution and variability and to optimize sampling criteria. The analysis did not indicate the presence of significantly high levels or consequently significantly high risks; nevertheless, it pointed out some important aspects in data collection and evaluation. First, it appeared that the statistical distribution of data is typically nonsymmetrical, non-Gaussian, and characterized by a tail extending toward the high values. The logarithmic transformation of data appeared useful in order to obtain a Gaussian distribution. As a rule, the data variability appeared to be high. The frequency and distribution of values above the analytical threshold resulted in significant change, both in time and in space. The percentage of positive values (above the analytical threshold) appeared to vary up to a factor of 2-3, as analogously did their geometric mean.(ABSTRACT TRUNCATED AT 250 WORDS)

Environmental Monitoring↗
PubMed1988

Dose-response relationships in rodents of promoter carcinogens: a tentative interpretation of some downward trends.

This study analyzes a set of dose-response curves relative to promoter carcinogens administered to experimental animals with or without initiator pretreatment. In the case of initiator plus promoter treatment, as well as for low experimental doses, the experimental data and their mathematical fitting indicate a downward shape of the dose-response curves as a general feature. In this study, the following hypothesis, according to the two-stage carcinogenic action of initiator plus promoter treatment, is presented to interpret this downward shape: (i) an initiating action, involving a subset of available "targets" (the number of "initiated" targets is assumed to depend on initiating treatment level), and (ii) a subsequent dose-dependent promoting process, mainly involving the initiated targets. This process reaches a saturation level when all the initiated targets have been promoted. This kind of process may be quantitatively described by a saturated exponential model: P(d) = K(1 - exp(-K0 + K1d)] which fits the experimental data fairly well. No indication supporting the hypothesis of a threshold for the chemicals examined resulted from the analysis. In the specific case of sodium saccharin, experimental dose-response relationships were available with or without initiator pretreatment (BBN and MNU). While in the first case the shape of the dose-response curve is downward, in the second case, it is upward and significantly different.

Animals↗
PubMedDate not supplied

State specificity of DST abnormalities in geriatric depression.

Pre-treatment and posttreatment dexamethasone suppression test (DST) results in physically healthy elderly major depressives without dementia demonstrated an association between treatment and DST normalization. Sixty percent of subjects were nonsuppressors at baseline compared to 17% after intensive treatment. DST results normalized in 75% of initial nonsuppressors; none of the initial suppressors converted to nonsuppression. A strong correlation between clinical improvement and decreases in afternoon cortisol levels was identified. Initial suppression status did not influence this association. There was a nonsignificant trend for very high (> 15 micrograms/dl) afternoon cortisol levels to be associated with delusional depression. The advantage of using continuous rather than categorical measures to assess the relationship between reversal of depression and DST results is discussed.

Aged↗
PubMedDate not supplied

[Endometriosis with ascites and pleural effusion: a case report].

Massive ascites associated with pelvic endometriosis is an uncommon combination. Pleural effusion may rarely occur. Fourteen cases are reported in the literature. We report the case of a black nulligravida woman followed for a primary infertility. Endometriosis was suspected in presence of increasing dysmenorrhea, cystic adnexal masse, umbilical nodes and ascites. The diagnosis was confirmed at exploratory laparoscopy. The patient had been followed for assisted procreation for six years and had undergone a conservative laparoscopic surgery never described. After several ascites recurrences, the pathology was resolved by Gonadotropin-releasing hormone agonist therapy. But a right pleural effusion with ascites occurred following a bad therapeutic observance. This complication reveals an early pregnancy never reported for this exceptional pathology. A conservative management allowed this unique case of well outcoming pregnancy. The possible pathogenesis of ascites and pleural effusion are explored and recommendations for diagnosis and treatment options are discussed.

Adult↗
PubMedDate not supplied

Clinical evaluation of patients with squamous carcinoma of the oral cavity.

AIMS: To make a clinical evaluation of patients with squamous carcinoma of the oral cavity. METHODS: The clinical condition of 72 patients with squamous carcinoma of the mouth, hospitalized at the Institute of Oral and Maxillo-Facial Surgery, 2nd University of Naples, Italy, between 1992 and 1996, were examined. The patients were evaluated system by system, staged by TNM-UICC and classified by ASA and by the Karnofsky and Burchenal and the Zubrod et al. Performance status methods. RESULTS: Evaluation by the two methods produced different results. Of the patients examined, 34.5% showed more than one systemic disorder. 51.4% were in ASA classes 3-4. CONCLUSIONS: The presence of a disease with poor prognosis associated with advanced neoplastic stage (stages III-IV; 69.4% of the total) precluded radical surgery in 32% of patients, who were treated with chemotherapy and/or radiotherapy.

Adult↗
PubMedDate not supplied

The use of hydrogen peroxide in the experimental therapy of cysts. An in vitro analysis.

AIM: Aim of the authors is to evaluate in vitro the histological alteration caused by hydrogen peroxide on a sample of radicular cysts. METHODS: Twelve radicular cysts were divided into 2 portions. The control group was histologically evaluated whereas the other portion, test, was treated with 3% hydrogen peroxide before histological analysis. Test and control portions were compared for integrity of the epithelium, inflammatory state of the cystic walls, presence of necrosis, stromal vacuolization and microvessel density (MVD). MVD was evaluated by immunohistochemistry using anti-CD34 monoclonal antibodies. RESULTS: Significantly lower epithelium integrity has been detected in the test group (p= or <0.007), (Wilcoxon test). Moreover, the test group presented a higher number of stromal vacuolization areas (p= or <0.004). However, necrosis and inflammation reported no significative differences. No significative differences were noticed for the MVD. CONCLUSION: Hydrogen peroxide showed a cytopathic and cytolytic activity in vitro. Those effects have been shown on both cysts tissues: epithelium and connective tissue. The present study did not reveal any statistically significant difference between the number of vessels in the control and in the test group. However, further in vitro and human studies are needed to validate this substance in the treatment of cysts.

Blood Vessels↗
PubMedDate not supplied

In vitro effects of different concentration of PRP on primary bone and gingival cell lines. Preliminary results.

AIM: Platelet rich plasma (PRP) is utilized in oral surgery to enhance bone healing and it has been suggested to accelerate soft tissue healing. Nevertheless, there is no evidence on biological concentration of platelets needed to determine the higher biological response. Therefore, aim of this study is to evaluate the action of PRP in vitro on osteoblasts and fibroblasts and to evaluate which is the most effective concentration of PRP. METHODS: PRP was obtained from volunteers donors by standard apheresis. Osteoblasts were growth for 72 hours in a medium added with platelet concentration of 230%. Fibroblasts were treated with different platelet density for 24 hours and 72 hours. Platelet density was increased of 230%, 350%, 460% and 700% the normal blood count. Cell proliferation was evaluated with MTT test. ANOVA test was used to assess cells proliferation data. RESULTS: Osteoblasts proliferation, at 72 hours, showed an increase of proliferation in PRP group compared to plasma (P < 0.001). Fibroblast proliferation after 24 hours increases when PRP is added (P < 0.05). However, no significant differences were detected among the various concentration of PRP. Yet, at 72 hours, MTT values increases when platelet concentrate is 230% and 350%. At 700% platelet density MTT values were lower than control group (P < 0.05). CONCLUSIONS: Our results indicated that PRP has an enhancing effect on osteoblasts and fibroblasts proliferation when it is prepared only within certain ranges of concentrations. However, further experimental studies are needed to confirm our hypothesis.

Adult↗
PubMedDate not supplied

Periodontitis: from local infection to systemic diseases.

Periodontitis is a chronic infectious disease, characterized by the progressive loss of the teeth's supporting tissues, affecting almost 40% of the entire adult population. An imbalance between a localized gram-negative infection and an exaggerated host inflammatory response plays a pivotal role in determining gingival tissue damage. Recent evidence suggests that the effect of periodontitis might not be limited just to the oral cavity but it might have systemic consequences. Indeed periodontitis has also been associated with a moderate systemic inflammatory response. Although the mechanisms behind this association remain unclear, periodontitis might represent one distant source of low-grade systemic inflammation. This association could explain the increased risk of future cardiovascular diseases observed, the impaired metabolic control in diabetes subjects and the adverse pregnancy outcomes observed in populations suffering from periodontitis. In this review we describe the pathophysiological processes involved in periodontitis and briefly review the evidence produced to support an association between periodontitis and systemic diseases.

Acute-Phase Reaction↗
PubMedDate not supplied

Parotid sialography and lip biopsy in the evaluation of oral component in Sjögren's syndrome.

In order to evaluate their sensitivity and specificity, parotid sialography (PS) and labial salivary gland biopsy were performed in 150 putative patients with Sjögren's syndrome (SS) (74 with SS alone and 76 with SS associated with other well-defined disorders). Although PS and LSGB strongly correlated to each other (Kendall's Tau 0.47, z = 7.78, p less than 0.0001), PS confirmed the diagnosis of SS in a significantly higher prevalence of patients with respect to LSGB (63% vs 37%, chi 2 = 18.25, p less than 0.001). When the sensitivity and specificity of PS and LSGB were calculated, PS showed a better sensitivity, and an only slightly lower specificity than LSGB (86% vs 70% and 78% vs 86% respectively). In conclusion, our study indicates that PS has been unduly overlooked in the past decades as a diagnostic tool for the salivary component of SS, and should be reconsidered as a potentially useful and relatively non-invasive procedure in the approach of patients with this disease.

Adult↗
PubMedDate not supplied

Statistical evaluation of different clinical and serological parameters for the diagnosis of Sjögren's syndrome.

The potential value for the diagnosis of Sjögren's syndrome (SS) of 28 clinical and 18 serological parameters was evaluated in 38 patients with primary (1 degree) SS, 29 with rheumatoid arthritis (RA), 30 with systemic lupus erythematosus (SLE), and 22 with mixed cryoglobulinemia (MC), by means of a stepwise discriminant analysis. Twelve patients with RA, 11 with SLE, and 8 with MC had evidence of sicca syndrome and were then classified as having a secondary (2 degree) SS. When comparing the various disease groups to each other, it was always possible to select different combinations of clinical and serological variables which defined highly significant discriminant functions. In addition, some parameters appeared to have a stronger discriminant power between different disease groups. For instance, 1 degree SS was well discriminated from RA by anti-Ro antibodies (Ab) and normal C-reactive protein (CRP) levels, from SLE by normal complement levels and the positivity of rheumatoid factor, from MC by the absence of liver involvement and normal complement levels. While anti-La Ab had the highest value in differentiating patients with 1 degree from those with 2 degrees SS, anti-Ro Ab and recurrent parotid swelling seem to be typical features of patients with SS with respect to those with other connective tissue disease but without sicca syndrome.

Adult↗