Biomedical subjects

M G Sevast'ianova

Publications and source records attributed to M G Sevast'ianova.

At least 19 recordsLinked to original sources

PubMed1991

[Oxygen-binding properties of blood in hemoglobinosis M Boston detected in the USSR for the first time].

Hemoglobin M-Boston comprising 30% of the total blood Hb was detected in two members of a Russian family. The stages of its identification were described. An analysis was made of probands' whole blood oxy-Hb dissociation curves. At pO2 = 100 mm Hg oxygenation of Hb M-Boston beta-chains was 44% only, while mutant alpha-chains were completely oxidated. Despite a significant decrease in arterial blood oxygen content arteriovenous variation by O2 content at pO2 differential from 100 to 40 mm Hg is within the normal level due to various directions of changes in P50 and Hill's coefficient of probands' whole blood. The authors consider that pronounced cyanosis in Hb M-Boston carriers is not the consequence of tissue hypoxia but is caused by a higher content of nonfunctioning Hb M-Boston that changes the blood color.

Adolescent↗

PubMed1990

[Antigen, common for mature human B- and T-lymphocytes, detected with the use of autolymphocytotoxins].

The investigation of adsorption capacity of B- and T-lymphocytes in respect to autolymphocytotoxic antibodies (ALCTA) has shown that these two populations of mature cells have a common antigen against which ALCTA are directed. This antigen is insufficiently expressed of pathologic cells of patients with B-cellular variant of chronic lymphoid leukemia (CLL). B-lymphocytes of patients with malignant non-Hodgkin's lymphoma, hairy-cell leukemia, Waldenström's macroglobulinemia are more mature, as compared to those of CLL patients, and carry surface antigens to which autoantibodies are directed.

Antigen-Antibody Reactions↗

PubMed1990

[Isolation, identification and electrophoretic properties of DNA excreted by human lymphocytes].

It was shown that the buoyant density of DNA isolated from lymphocyte incubation media as well as from blood plasma of healthy patients and patients with chronic lympholeukemia (CLL) using the SDS-phenol method with subsequent ultracentrifugation in a cesium trifluoroacetate density gradient is 1.59-1.60 and 1.60-1.63 g/ml, respectively. Using electrophoresis in 0.6% agarose gel, it was found that the DNA excreted by lymphocytes from healthy patients and CLL patients is a high molecular weight fraction comprising 21,000 nucleotide pairs. This DNA fragment contains 90-95% of [3H]thymidine used for the labeling of DNA excreted by lymphocytes from healthy patients and patients with CLL. No traces of [3H]thymidine were found in the middle part of the agarose gel containing the diffuse material bound to ethidium bromide.

DNA↗

PubMed1989

[Characteristics of hematopoiesis in patients with the leukopenic syndrome].

The authors present data concerned with a long-term (up to 20 years) prophylactic medical observation over 103 patients with pronounced stable leukopenia. The different pathological processes associated with the development of the leukopenic syndrome were identified as were the reactive shifts in the medullary hemopoiesis of varying intensity. The shifts turned out nonspecific for all the disease entities under study.

Bone Marrow↗

PubMed1987