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Biomedical subjects

M G Mott

Publications and source records attributed to M G Mott.

At least 55 records · Page 3Linked to original sources
PubMed1989

Loss of chromosome 11p alleles in cultured cells derived from Wilms' tumours.

Cell cultures have been produced from five Wilms' tumours. All cultures had a finite lifespan and a pattern of antigen expression which indicated that the cells were derived from the differentiated components of the tumours. No cells showed any of the expected characteristics of the putative Wilms' tumour stem cell. Nevertheless, in both cases where the original tumours showed a loss of heterozygosity at chromosome 11p alleles, the cultured cells also demonstrated a loss of heterozygosity. Thus these cell cultures definitely originated from Wilms' tumour tissue. The results demonstrate that cell cultures can be produced from the differentiated tissues present in Wilms' tumours and that these non-immortal cells show no 'transformed' phenotype, even though they possess the genetic changes present in the original tumour.

Alleles↗
PubMed1989

Thyroid function in survivors of cancer.

Thyroid function was assessed in three selected groups of children who had survived cancer. Children in group 1 had received radiotherapy to the thyroid area, group 2 had radiotherapy to the thyroid area and adjuvant chemotherapy, and group 3 had chemotherapy with or without radiotherapy away from the thyroid area. There were 75 survivors and 63 (40 boys, 23 girls) were available for study. Eighteen (29%) were found to have thyroid dysfunction, and these included all those who had had lymphangiograms or received a radiation dose greater than 40 Gy to the thyroid area. Only nine of the 18 children were already known to have thyroid dysfunction, and only 15 of 44 children who had had irradiation to the thyroid area had had their thyroid function examined. This study shows that children who have received radiotherapy to the thyroid area should have their thyroid function assessed regularly. Chemotherapy does not appear to be a risk factor but longer follow up of these children is necessary.

Adolescent↗
PubMed1988

Paediatric oncology information pack for general practitioners.

An information pack covering important aspects of paediatric oncology has been developed for general practitioners. Sixty general practitioners who received the information pack found that it helped them in managing children with neoplastic disease and their families. The pack has also improved communications between the oncology unit and general practitioners. Similar packs could be produced by paediatricians working in other specialties.

Child↗
PubMed1988

Bacteraemia related to indwelling central venous catheters: prevention, diagnosis and treatment.

Infective episodes in immunocompromised children with indwelling central venous catheters were studied prospectively for one year. Culture of catheter hubs and skin at catheter entry sites during the first six months suggested that hub contamination was important in the pathogenesis of catheter colonization. The incidence of catheter-related bacteraemia, and possible catheter-related bacteraemia, fell by 56.5% following alterations in the protocol for manipulative care of catheters, from 5.82 per 1000 catheter days in the first six months to 2.53 per 1000 catheter days in the subsequent six months. A firm diagnosis of catheter-related bacteraemia was made simply and economically by a pour-plate quantitative blood culture technique. Attempts at eradication of catheter-related bacteraemia without removal of the catheter were successful in all cases.

Adolescent↗
PubMed1987

Second malignancies in children treated for non-Hodgkin's lymphoma and T-cell leukaemia with the UKCCSG regimens.

Eight children treated between 1977 and 1983 with the UK Children's Cancer Study Group's non-Hodgkin lymphoma (NHL) and T-cell protocols have developed second malignancies within 7 years of commencing treatment. Five developed acute non-lymphoblastic leukaemia and a sixth died from infection while pancytopenic with a pre-leukaemic marrow. The other malignancies were cerebral astrocytoma and an undifferentiated low grade sarcoma. These eight children were included among 261 children studied in the first UKCCSG NHL and T-cell trials giving an actuarial incidence of 7.8% second malignancy at 7 years. Six had received adjuvant radiotherapy which may have contributed to the high incidence of second malignancy.

Adolescent↗
PubMed1987

Hybrid leukaemia of T cell and myeloid lineages: cytogenetic distinction from second (induced) malignancy.

The therapeutic and prognostic implications of relapse and clonal evolution of leukaemia are substantially different from those of secondary (induced) malignancy. This report documents the case of a patient who presented with apparent acute non-lymphocytic leukaemia (ANLL) following therapy for acute (T-cell) lymphoblastic leukaemia (ALL) 4 years previously. Morphologically and cytochemically the cells were of myeloid type, but the cell markers showed a T cell lineage. Cytogenetic studies confirmed that this was a relapse of T cell ALL with a phenotypic change, rather than a second malignancy induced by chemotherapy. A 14q deletion present at initial diagnosis recurred at relapse, with the addition of cells with complete deletion of chromosome 14, indicating clonal evolution.

Child↗
PubMed1986

Burkitt-like lymphoma in an English child: characterisation of tumour biopsy cells and of the derived tumour cell line.

An eight year old English boy presented with an abdominal undifferentiated 'Burkitt-like' lymphoma. Lymphoma cells from ascitic fluid were cultured on a human embryo fibroblast feeder layer and, after a short lag period, a cell line (DH-BL) was established which, like the original tumour, was both negative for the Epstein-Barr nuclear antigen (EBNA) and expressed a monoclonal pattern of surface immunoglobulin (alpha lambda). DH-BL also possessed the Burkitt-related 8:14 chromosome translocation in all metaphases analysed; no other chromosomal abnormalities were present. The cell surface phenotype of the original biopsy cells and the cultured tumour cells in early passage were investigated using a panel of monoclonal antibodies to B lineage-associated antigens. These antibodies had recently been used to characterise African 'endemic' Burkitt's lymphoma (BL) biopsy cells and their derived cell lines. The cell surface phenotype of this English EBNA negative Burkitt-like lymphoma biopsy was indistinguishable from that previously shown by biopsies of EBNA positive endemic BLs. It therefore appears that both the endemic and sporadic forms of BL, as illustrated by this case, may be derived from the same subset of progenitor cells.

Abdominal Neoplasms↗
PubMed1986

Fungal pneumonias masquerading as thromboses during induction therapy of acute lymphoblastic leukemia.

Fatal fungal pneumonias developed in two children with ALL during remission induction. Pulmonary and central nervous system signs suggested the L-asparaginase-induced multiple-thromboses syndrome in both children. Fungal infection should be considered-whenever pneumonia develops in a neutropenic child already receiving broad-spectrum antibiotics or when multiple thromboses occur.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1985

Monocytic leukemia in infancy. A review of eight children.

The clinical and pathologic features of eight infants with monocytic leukemia are reviewed. The children were all aged 12 months or less at diagnosis and had a high incidence of extramedullary features, skin infiltration being particularly common. The diagnosis was established by conventional morphologic and cytochemical techniques. Using the French-American-British (FAB) classification, five infants had FAB 5a disease, and three had FAB 5b. The difficulties in making the diagnosis from extramedullary sites and the overlap that exists at this age between monocytic leukemia, true histiocytic lymphoma, and malignant histiocytosis are discussed. The treatments given to the group and their response are reviewed. Five of the patients received VP-16-213 and cyclophosphamide as primary induction chemotherapy, a combination that merits further evaluation in leukemia with monocytic features.

Female↗
PubMed1984

Effective chemotherapy for metastatic malignant sacrococcygeal tumour.

Two children with disseminated malignant sacrococcygeal tumour were treated with six courses of vinblastine, cis-platinum, and bleomycin. Both had exploratory operations after four courses of treatment and no histological evidence of malignant disease was found. The two children have remained free of disease with normal alpha-fetoprotein levels for 30 months and 18 months, respectively.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1984

Modification of oral methotrexate absorption in children with leukemia.

The effect of administering oral methotrexate in different formulations to children with acute lymphoblastic leukemia was evaluated. Methotrexate tablets alone achieved higher mean plasma levels and larger area under the absorption curve than either methotrexate liquid alone or methotrexate tablets taken concurrently with metoclopramide.

Absorption↗
PubMed1984

Adjuvant low dose radiation in childhood T cell leukaemia/lymphoma (report from the United Kingdom Childrens' Cancer Study Group--UKCCSG).

From November 1977 to July 1983, 82 children with T leukaemia/lymphoma entered a randomised trial of combination chemotherapy and radiotherapy. Twenty-five were designated T lymphoma and 57 T leukaemia, 28 having greater than 100 x 10(9)1(-1) blasts in peripheral blood at diagnosis. Twenty-seven patients with mediastinal primaries who were treated on the companion non-Hodgkin lymphoma (NHL) trial were comparable in all respects to the T lymphoma patients and the results of treatment were therefore combined and analysed together. Overall 4-year survival (48-53%) and failure-free survival (FFS) (37-40%) were similar in all groups except the 28 with T leukaemia and WCC greater than 100 X 10(9)1(-1) (20% and 13%). There was a significant advantage in FFS for patients randomised to receive low dose mediastinal radiation, and this was most marked in patients with T lymphoma (66% vs 18%, P = 0.006).

Adolescent↗
PubMed1984

Adjuvant low dose radiation in childhood non-Hodgkin's lymphoma (report from the United Kingdom Childrens' Cancer Study Group--UKCCSG).

From July 1977 to July 1983, 120 children with non-Hodgkin's Lymphoma entered a randomised trial of combination chemotherapy and radiotherapy. The primary site was abdominal in 42 patients, mediastinal in 27 and in other sites in 51. Failure-free survival (FFS) at 4 years was 74% for the 41 patients with localised disease (Stages I and II) and 51% for the 79 with generalised disease (Stages III and IV). Patients with mediastinal primaries continued to relapse after the completion of 2 years' treatment, but FFS at 4 years for the 93 patients with non-mediastinal primaries was 65% for all stages combined. In the latter group, there was no benefit to patients randomised at the end of induction chemotherapy to receive adjuvant radiation 15 Grays in 10 fractions in 2 weeks to sites of previous bulky disease when compared to those not receiving such radiation (P = 0.6).

Abdominal Neoplasms↗
PubMed1984

Cushing's syndrome and bronchial carcinoid tumour.

Adrenal function test results in a girl with Cushing's syndrome and a bronchial carcinoid tumour suggested pituitary dependent hypercortisolism. Resolution after excision of the tumour indicated that her condition had been caused by ectopic adrenocorticotrophic hormone secretion. Conventional tests of adrenal function may be misleading in cases of adrenocorticotrophic hormone secreting bronchial carcinoid tumours.

Adolescent↗