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M G Honig

Publications and source records attributed to M G Honig.

21 records · Page 2Linked to original sources
PubMed1984

Heterogeneity of group Ia synapses on homonymous alpha-motoneurons as revealed by high-frequency stimulation of Ia afferent fibers.

Excitatory postsynaptic potentials (EPSPs) were recorded in medial gastrocnemius (MG) motoneurons following intraaxonal electrical stimulation of single spindle afferent fibers in anesthetized cats. High-frequency bursts of 32 shocks were delivered to the afferent axon and the EPSPs were averaged in the motoneuron. EPSP amplitude generally changed during the burst, in some cases increasing and in other cases decreasing, depending on the connection. Interpretation of these changes was complicated by potentiation of the initial EPSPs in the burst that occurred with the repeated bursts. The extent of the potentiation varied from connection to connection. The magnitude of facilitation or depression during a burst of standard frequency (167 Hz) was determined by comparison of EPSPs at the end of the burst with the mean EPSP obtained during low-frequency stimulation (18 Hz). Large amplitude EPSPs tended to depress, whereas the small amplitude EPSPs facilitated. Facilitation was more prevalent in motoneurons with large rheobases and depression was more often observed in small rheobase motoneurons. The use of partial correlations, which was necessary because of the inverse correlation between EPSP amplitude and motoneuron rheobase, revealed that facilitation-depression behavior during repetitive stimulation is correlated primarily with EPSP amplitude rather than with motoneuron rheobase. Acute transection of the spinal cord resulted in no change in motoneuron rheobase but considerable enlargement of mean EPSP amplitude at low frequencies of stimulation. A significant increase in the amount of depression during repetitive stimulation was noted under these conditions. These results indicate considerable heterogeneity in the response of individual connections to repetitive stimulation. We suggest that this heterogeneity results from differences in transmitter release at different connections. This heterogeneity must also have functional consequences related to susceptibility for firing of different motoneurons under various physiological conditions that can include afferent discharge frequencies equivalent to those used in this study.

Afferent Pathways↗
PubMed1982

The development of sensory projection patterns in embryonic chick hind limb.

1. The distribution within individual dorsal root ganglia (d.r.g.s) of sensory neurones projecting to different targets in the embryonic chick hind limb was determined using the retrograde transport of horseradish peroxidase (HRP). The segmental pattern of sensory neurone projections was also defined, using retrograde and orthograde HRP labelling and electrophysiological techniques, from the onset of axonal outgrowth into the limb until after the period of sensory cell death. 2. At stage (St.) 29-30, shortly after initial axonal outgrowth into the limb, the large lateroventral neurones in the d.r.g.s projected both to skin and to muscle. At St. 36-37, after cell death in the d.r.g.s, cells from both the lateroventral and mediodorsal populations projected both to skin and to muscle. Thus these two cell populations do not correspond to cutaneous and proprioceptive afferents, respectively. 3. The cells projecting along individual cutaneous nerves or to individual muscles were always widely distributed throughout the ganglia. Thus, sensory neurones cannot be specified to project to particular peripheral targets as a result of their position in the d.r.g. Nevertheless, small clusters of cells were frequently found to project along the same peripheral nerve. 4. Since there is a correlation between position in the d.r.g. and time of origin, neurones projecting to each target have a wide range of birthdates, and, therefore, sensory neurones cannot be specified as a result of their birthdate. 5. At St. 36-38, after cell death, afferents to a given muscle or cutaneous nerve arise primarily from two or three adjacent segments out of the eight lumbosacral segments. For muscles these are the same segments that supply the motoneurones to that muscle. Each d.r.g. sends a characteristic proportion of axons down each of several peripheral nerves in a consistent and orderly pattern. 6. During initial outgrowth, the segmental projection pattern is similar to the pattern found in mature embryos. Thus, extensive projection 'errors' are not made and neither cell death nor retraction of axons is necessary for establishing the appropriate connectivity pattern. The majority of neurones do not send branches down more than one peripheral nerve. 7. Axons projecting to the same target are initially dispersed in the spinal nerves, and gradually segregate out in the plexus region, ultimately to form a separate nerve trunk. Axons projecting to different targets cross each other. Cutaneous and muscle nerves first form at the same stages. Therefore, the particular pathways axons take do not depend in any simple way on either axonal position in the plexus or time of arrival at the base of the limb. Simple timed outgrowth mechanisms and models in which axons maintain constant topographical relationships with each other therefore cannot generate the observed projection pattern. 8...

Animals↗