Search PubMed⌕ Search

Biomedical subjects

M G Canese

Publications and source records attributed to M G Canese.

34 records · Page 2Linked to original sources
PubMed1976

Complement fixing hepatitis B core antigen immune complexes in the liver of patients with HBs antigen positive chronic disease.

One hundred and fifty-two biopsies from serologically HBsAg positive and negative patients with liver disease were studied in immunofluorescence: for the presence of the surface (HBs) and the core (HBc) antigenic determinants foeterminants of the hepatitis B virus, of immunoglobulins and complement (C) deposits, and for the capacity to fix human C. Circumstantial evidence is presented suggesting that HBc immune-complexes are a relevant feature in the establishment and progression of chronic HBSAg liver disease. C fixation by liver cells was shown in all HBC positive patients with chronic hepatitis; an active form was present in every case, except two with a persistent hepatitis, an inverse ratio of HBc to C binding fluorescence being noted between active chronic hepatitis and cirrhotic patients. HBc without C fixation was observed in only three patients in the incubation phase of infectious hepatitis. IgG deposits were often found in HBc containing, C fixing nuclei. No C binding or IgG deposits were observed in acute self-limited type B hepatitis, in serologically positive patients with normal liver or minimal histological lesions, with and without HBs cytoplasmic fluorescence in their biopsy, or in serologically negative individuals.

Adolescent↗
PubMed1973

Human peripheral lung tumours: light and electron microscopic correlation.

Thirteen human peripheral lung tumours have been studied in both light and electron microscopy. They were classified as epidermoid carcinoma, mucus-secreting cell adenocarcinoma, and alveolar cell adenocarcinoma, the latter made up of granular pneumocytes. Alveolar cell cancer, as defined by ultrastructural features, could assume different gross histological patterns in light microscopy, and therefore electron microscopy is required for its identification.Since neither squamous nor mucous metaplasia was observed in any alveolar cell tumour, it is tentatively suggested that all peripheral lung tumours which lack these features may be derived from granular pneumocytes, irrespective of whether they appear to be adenocarcinomata or large cell carcinomata when examined by light microscopy.

Adenocarcinoma, Bronchiolo-Alveolar↗
PubMedDate not supplied

Experimental HBV and delta infections of chimpanzees: occurrence and significance of intrahepatic immune complexes of HBcAg and delta antigen.

The occurrence and pathogenetic role of intrahepatic deposits of immunoglobulins in experimental viral infection have been evaluated by determining with immunofluorescence their capacity to fix complement in vitro [in vitro complement fixation (VCF)]. Liver biopsies from chimpanzees chronically or acutely infected with hepatitis B virus or the hepatitis B surface antigen (HBsAg)-associated delta agent were used in the study. VCF was observed in each animal expressing hepatitis B core antigen (HBcAg) or delta antigen in the liver and concurrently circulating the homologous antibody in the blood. In acutely infected animals, VCF appeared at the same time that the homologous serum antibody appeared, and the intensity of VCF staining was proportional to the antibody titer in the serum. In animals expressing sequentially the HBcAg/antibody system and then delta antigen and antibody to delta, VCF was first observed in HBcAg-containing nuclei and then in nuclei expressing delta antigen. There was no relationship between VCF and intrahepatic expression of HBsAg or serologic expression of hepatitis B e antigen (HBeAg). A positive VCF reaction appears related to the formation of intrahepatic immune complexes between HBcAg or delta antigen and the homologous antibody. Although acute hepatitis developed in parallel with the occurrence of VCF in two animals, strong VCF fluorescence was also observed in each of the asymptomatic carriers of HBsAg, and, in one of them, preexisting VCF staining of HBcAg disappeared in parallel with development of acute hepatitis. In experimentally infected chimpanzees, the finding in liver biopsies of immune complexes detectable by VCF appears to be a common epiphenomenon without pathogenic significance.

Animals↗
PubMedDate not supplied

Identification of beta-adrenoceptor subtypes in bovine ovarian and myometrial cell membranes.

Beta-adrenoceptor (beta-AR) concentrations were measured in the ovary and in the myometrium of 36 adult Friesian cows using a radiometric assay. The beta-AR content in both tissues was determined using the highly specific antagonist (-) [3H]CGP 12177 and the amounts of beta-AR subtypes were discriminated in the presence of highly selective unlabelled ligands (CGP 20712A, ICI 118 551, CGP 25827A). Scatchard analysis revealed a good linearity and Kd values suggested the existence of high affinity beta-adrenergic sites in the bovine genital tract. Total beta-AR concentrations in the ovary and in the myometrium were, respectively, 87 +/- 7 (SEM) and 240 +/- 27 fmol mg-1 of membrane protein. beta 2-AR concentrations were significantly higher (P < 0.01) in the ovary (66 +/- 5) and the myometrium (180 +/- 29) than those of beta 1-AR (21 +/- 4 and 60 +/- 5, respectively). Significant differences (P < 0.05) were also to be found between high affinity state beta 2-AR and low affinity beta 2-AR concentrations, but their values correlated negatively in the two different tissues. Natural and synthetic agonists inhibited (-) [3H]CGP 12177 binding to beta-AR in the following order of potency: (-)isoproterenol > (+/-)clenbuterol > or = (-)adrenaline >> >> (-)noradrenaline, whereas synthetic antagonists inhibited binding in the following order of potency: (-)propranolol >> (+/-)ICI 118 551 >> >> (+/-)CGP 20712A.

Animals↗
PubMedDate not supplied

Experimental transmission of a sarcosporidian from Alpine ibex to domestic sheep and goats.

Sporocysts of Sarcocystis sp. from dogs fed with ibex meat were orally inoculated into kids and lambs. Three kids, given 4 x 10(6) and 4 x 10(4) sporocysts, respectively, died from acute sarcocystosis. Schizonts, though found in all the tissues of these kids, were particularly numerous in the kidneys, brain and spinal cord. Another three kids inoculated with 5 x 10(3) sporocysts and two lambs, inoculated with 1 x 10(6) and 5 x 10(3) sporocysts, respectively, showed no clinical signs and were sacrificed between 111 and 130 days after infection. Mature sarcocysts were found both in the heart and striated muscles of these animals. No parasitic stage was found in two kids and two lambs used as uninoculated controls. Biological differences between Sarcocystis sp. from ibex and the other sarcosporidians with a canine-caprine or canine-ovine cycle are stressed.

Animals↗