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Biomedical subjects

M Furuya

Publications and source records attributed to M Furuya.

At least 145 records · Page 8Linked to original sources
PubMed1985

[A review of experimental thermochemotherapy in cultured cells].

Recent studies and reports on interactions of heat and drugs are somewhat scattered both in the temperatures and types of drugs adopted. In the present review, the current skate of knowledge regarding experimental thermochemotherapy and thermotolerance and heat shock proteins was evaluated. Hyperthermia is not only interesting as an adjuvant therapy but is also expected to be developed as an independent modality among oncotherapies available at present.

Animals↗
PubMed1985

[Clinical significances of tissue polypeptide antigen in patients with gynecological malignancies].

Recently, tissue polypeptide antigen (TPA) has become of general interest as one of the new tumor-related antigens. In this study, TPA was measured mainly in patients with various gynecological malignancies by radioimmunoassay, and serum TPA levels above 110u/l were considered pathological. The serum TPA level in normal women was 66 +/- 21 u/l (mean +/- S.D.) and the positive rate was 0 percent. Serum TPA levels in patients with cervical cancer, endometrial cancer, ovarian cancer and uterine sarcoma (181 +/- 262, 117 +/- 49, 217 +/- 215, 142 +/- 49u/l) and positive rates (55, 40, 53, 75 percent) were significantly higher than those in normal women. Serum TPA levels in patients with advanced or recurrent diseases had a tendency to show highly positive. In all of 13 patients with positive TPA, serum TPA levels were significantly reduced and turned negative after the appropriate treatments. However, among our follow-up patients, serum TPA levels were often positive even when there was no clinical evidence of recurrence. Conclusion; serum TPA measurement could be a useful subordinate tumor marker in many patients with gynecological malignancies, even though careful judgement is necessary to interpret positive TPA.

Adenocarcinoma, Mucinous↗
PubMed1985

Immuno-histological localization of tissue polypeptide antigen (TPA) in gynecological malignancies.

The presence of Tissue Polypeptide Antigen (TPA) was demonstrated by immunohistochemical techniques in various specimens of gynecological malignancies as well as uterine precancerous lesions and trophoblastic disease. In 82 cases, the overall positive rate for staining was 49%. The relationship between the histological progress of malignancy and the positive rate for TPA was not confirmed, nor was the positive rate directly proportional to the histological typings. A serum TPA level of 160 U/l was considered to be the critical level of immunohistological positivity.

Adenocarcinoma↗
PubMed1984

Trypanosoma gambiense: immunosuppression and polyclonal B-cell activation in mice.

The relationship between the suppression of antibody response and polyclonal B-cell activation was studied in mice treated with a cell homogenate of Trypanosoma gambiense. The cell homogenate injection in mice caused a progressive increase in splenic background plaque-forming cell response to sheep erythrocyte. In the mice with markedly increased background plaque-forming cell response, the different reactivity in the primary antibody response to sheep erythrocytes was observed between the intraperitoneal and intravenous immunization with sheep erythrocytes. The intraperitoneal immunization of mice with sheep erythrocytes strongly suppressed the antibody response, while the intravenous immunization with sheep erythrocytes led to an enhancement of the antibody response. The intraperitoneal injection of silica particles, a toxic agent to macrophages, 30 min before intraperitoneal immunization with sheep erythrocytes abolished the suppression of the antibody response completely. In addition, restoration of the suppressed antibody response was found in mice immunized intraperitoneally with a high dose of sheep erythrocytes. It appears that the suppression of antibody response is not attributable to polyclonal B-cell activation, and is associated with the elevation of the phagocytic activity of peritoneal macrophages.

Animals↗
PubMed1984

Effects of bleomycin on kinetoplast DNA and nuclear DNA in Trypanosoma gambiense using in situ microfluorometry technique.

The effect of Bleomycin, an anti-tumour antibiotic, on either kinetoplast DNA(K-DNA) or nuclear DNA(N-DNA) in a single Trypanosoma gambiense cell was measured successfully utilizing the in situ microfluorometry technique. This microfluorometric assay clearly revealed that the relative fluorescence intensity (RFI) of K-DNA and N-DNA was greatly reduced by bleomycin, suggesting the occurrence of the single strand breaks in these DNAs. The present results have shown that bleomycin acts on N-DNA stronger than K-DNA at least in Trypanosoma gambiense. Microfluorometry technique can be employed valuably for studying the DNA damages of several anti-protozoan drugs.

Animals↗
PubMed1984

Antimicrobial activity of Obioactin.

A newly developed enzyme-hydrolyzed toxoplasma-hyperimmune bovine serum preparation, Obioactin , was examined for its anti-microbial and related actions against heterologous protozoa, bacteria, and viruses both in-vivo and in-vitro. An appreciable efficacy of Obioactin in a combination with nifurtimox, Lampit was observed in experimental Trypanosoma cruzi infections in mice. But prophylactic applications of the agent alone or joint with Lampit were in vain in mice. In-vitro digestion of certain bacteria in mouse peritoneal macrophages was accelerated in the presence of the agent being three to ten times stronger than that in untreated macrophages. Inhibitory effect of Obioactin on in-vitro infectivity of viruses was suggested.

Animals↗
PubMed1983

Suppressive effect of bradykinin to cellular immune responses in vivo and in vitro.

Bradykinin, as well as histamine, one of the mediators in IgE mediated immediate type allergic reactions or acute inflammation, may affect the in vitro and in vivo cell-mediated immune reactions of the immunized animals. It was demonstrated in our present experiment that the appearance of delayed type hypersensitivity (DTH) skin reaction in the immunized guinea pig was remarkably suppressed by treatment of bradykinin or histamine and the suppression of cutaneous DTH by bradykinin was inhibited by H-2 antagonist (burimamide) but not by H-1 receptor blocker (chlorpheniramine). It was also clearly demonstrated that bradykinin suppressed the production of antigen-induced macrophage migration inhibitory factor (MIF) of the immune guinea pig peritoneal exudate cells (PECs) and the production of MIF was blocked by H-2 antagonist (burimamide) or H-2 agonist (tolazolin) but not by H-1 antagonist (chlorpheniramine). Antigen-induced lymphocyte proliferation of the immunized mice, one of the indicators of the cellular immune response, was also suppressed by treatment of bradykinin. These results indicate that bradykinin as well as histamine may have some role in the subsequent expression of cellular immune reactions.

Animals↗
PubMed1982

Effects of a triterpenoid saponin on spectral properties of undegraded pea phytochrome.

Soyasaponin I, a triterpenoid saponin isolated from etiolated pea (Pisum sativum cv. Alaska) shoots and identified as Pfr killer, was examined for its effects on spectral properties of undegraded pea phytochrome. When soyasaponin I in concentrations of 100 micromolar or lower was added to Pr in the dark, the spectrum of Pr was not significantly affected, whereas in the presence of 120 micromolar or higher concentrations the absorption maximum of Pr shifted from 666 to 658 nanometer with slight decrease of absorbance. After a brief exposure of the mixture to red light, the increase in absorbance at 666 nanometers that occurs in the dark was inhibited at 26 micromolar and higher soyasaponin I concentrations; the maximum effect being reached at about 180 micromolar. The decrease in absorbance at 724 nanometers in the dark after red light irradiation was somewhat inhibited by 60 micromolar and totally prevented by 410 micromolar soyasaponin I. When P(658) was irradiated with red light in the presence of 220 micromolar or higher soyasaponin I concentrations, a bleached form (P(bl)) was produced instead of Pfr. P(bl) showed no dark spectral changes, and the phototransformation of P(bl) to P(658) required a significantly high irradiance of far-red light. When the saponin was added to Pfr in the dark, none of the above-described spectral changes occurred, although the same effects were observed after the mixture was exposed briefly to far-red light followed by red light.

Journal Article↗
PubMed1981

Treatment of congenital pseudoarthrosis of the tibia by a free vascularized fibular graft: case report.

A free vascularized fibular graft was used to treat a case of congenital pseudoarthrosis of the tibia in a 5-year-old girl. Four weeks after the surgery, x-ray films revealed remarkable callus formation at the proximal junction of the graft and tibia. Seventeen months passed before union at this junction was completed. Union at the distal junction, on the other hand, took only 12 weeks. It was noted that the fibular graft enlarged in diameter rapidly, and at 17 months was the same size as the recipient tibia.

Child, Preschool↗
PubMed1981

Transmission and scanning electron microscopic studies of the micronemata of Trypanosoma gambiense.

The structure of micronemata arising from the surface of the bloodstream form of Trypanosoma gambiense was studied by electron microscopy. In order to produce micronemata, trypanosomes were incubated in either 1) phosphate buffered saline supplemented with glucose (PBSG), 2) immune mouse serum or 3) PBSG after passage through a DEAE-cellulose column. Electron microscopic examination of the parasite revealed the presence of thread-like micronemata arising from the anterior end and from the flagellar pocket regardless of the incubation conditions. Negative staining revealed a distinct peripheral fringe layer with nodular protrusions covering the entire surface of the micronema. The distribution and number of intramembrane particles (IMP) on the P and E faces of the micronema were similar to those of the flagellum of T. gambiense, indicating a close relationship between the membrane structure of the micronema and the flagellum. Micronemata became fragmented and adhered to each other after incubation of the parasite in the media for 12 h. Since micronemata tend to have the characteristics of adhesiveness and fragmentation, fragments of these structures might adhere to various host organs. Dispersal of potential antigenic material might be responsible, in part, for the induction of the host immune response.

Adhesiveness↗
PubMed1981

Agglutination antibody responses to Trypanosoma gambiense homogenate in mice treated with dextran sulfate 500 and carrageenan.

Dextran sulfate 500 and carrageenan, sulfated polysaccharides, have been considered as macrophage-toxic giving profound effects on the immune response. This work deals with agglutination antibody responses to T. gambiense homogenate in mice treated with dextran sulfate 500 and carrageenan. Antibody responses to the first immunization were suppressed in mice treated with the agents before the first immunization but reverse was the case with the response to the second immunization. These suppression and enhancement appeared to be dependent on the timing of treatment in regard to the time of the first immunization. The enhanced antibody response was abolished by either treating the mice with anti-thymocyte serum or transferring of spleen cells treated with anti-thymocyte serum into mice. Thus, the enhancement of antibody response is seemingly attributable for the most part to potent memory T cells induced by treatment with the agents.

Agglutination Tests↗
PubMed1981

In situ microfluorometry of whole kinetoplast and nuclear DNA in a single cell of Trypanosoma gambiense and Trypanosoma cruzi.

In situ microfluorometry of whole kinetoplast DNA (K-DNA) and nuclear DNA (N-DNA) from a single trypomastigote cell of Trypanosoma gambiense (strain Wellcome) and Trypanosoma cruzi (strain Tulahuen) was attempted by using the microfluorometer combined with a photon counter. The results obtained were summarized as follows; 1. The K-DNA value of 2K 1N (2 kinetoplasts and 1 nucleus) T. gambiense was about twofold as much as that of 1K 1N on. 2. The N-DNA value of 2K 1N T. gambiense was a little less than two times as much as that of 1K 1N T. gambiense, showing that the N-DNA had already bee increased nearly twofold before the nuclear division was initiated. 3. The K-DNA value of 1K 1N T. cruzi was about fivefold as much as that of 1K 1N T. gambiense. The N-DNA value was contrarily lower than that of T. gambiense, being approximately 70% of the latter. 4. Percent K-DNA/N-DNA value was, in average, about 7 in either 1K 1N or 2K 1N T. gambiense and about 50 in T. cruzi. 5. Percent K-DNA/N-DNA value in individual trypomastigote cell was variable, showing that the increase of K-DNA and N-DNA was not synchronized at least in the two species of trypanosomes used here. 6. The fact that K-DNA and N-DNA values varied from cell to cell although they do not divide, suggested the possibility of DNA increase in the trypomastigote of T. cruzi.

Animals↗