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Biomedical subjects

M Furue

Publications and source records attributed to M Furue.

At least 127 records · Page 7Linked to original sources
PubMed1994

Secondary cutaneous oxalosis: cutaneous deposition of calcium oxalate dihydrate after long-term hemodialysis.

A 48-year-old Japanese man had miliary calcified papules on the distal fingers after long-term hemodialysis. The crystals in the dermis consisted of calcium oxalate, as revealed by light microscopy and scanning electron microscopy with energy-dispersive x-ray microanalysis. The calcium oxalate was in the form of a dihydrate crystal. Therefore a diagnosis of secondary cutaneous oxalosis was made. In cutaneous oxalosis that occurs as a result of hemodialysis, miliary deposits occur in the fingers, but not the toes, and are present more frequently on the palmar aspects of the fingers. This localization may result from local differences in the metabolism or concentration of calcium or oxalate, or changes in the blood circulation.

Calcium Oxalate↗
PubMed1994

Bullous systemic lupus erythematosus with cutaneous mucinosis and leukocytoclastic vasculitis.

A 38-year-old man with systemic lupus erythematosus had nodular cutaneous mucinosis, leukocytoclastic vasculitis, and a vesiculobullous eruption. Immunopathologic study of a bullous lesion revealed granular deposits of IgA and IgG along the basement membrane zone. Localization of immune deposits to the lamina and sublamina densa was confirmed by immunoelectron microscopy.

Adult↗
PubMed1994

Immunosuppressive effects of azelastine hydrochloride on contact hypersensitivity and T-cell proliferative response: a comparative study with FK-506.

Azelastine hydrochloride (AZE) is an anti-allergic drug that inhibits the release of various chemical mediators from mast cells. We compared the immunosuppressive effects of AZE and FK-506 in vivo and in vitro. Topical application of AZE strongly inhibited the efferent phase of contact hypersensitivity, as did application of FK-506. In in vitro experiments, we found that 1) the suppression by AZE on interleukin (IL)-2 production from splenic T cells was partial and considerably large amounts of IL-2 were still produced, even in the presence of 10(-5) M of AZE, which was in sharp contrast to the observed marked inhibition of [3H]-TdR incorporation; 2) AZE significantly inhibited the phorbol myristate acetate-induced IL-2 responsiveness; 3) AZE did not inhibit the IL-2 receptor alpha expression of activated T cells; and 4) the significant inhibitory action was still observed even when AZE was added at 48 h after the initiation of culture. In regard to FK-506, we found that 1) FK-506 completely blocked the production of IL-2; 2) exogeneous IL-2 consistently restored the FK-506-induced inhibition; 3) FK-506 affected the phorbol myristate acetate-induced IL-2 responsiveness very little, if any; and 4) the significant suppression was observed only when FK-506 was added within 24 h after the initiation of culture. Thus, AZE exerts its in vitro immunosuppressive activity preferentially by interfering with the IL-2 responsiveness, with partial inhibition of IL-2 production. Conversely, FK-506 acts as a strong inhibitor of IL-2 production without a prominent effect on IL-2 responsiveness. The immunosuppressive activity of AZE shown in vitro may also be operative in vivo and may be applicable for topical use.

Administration, Topical↗
PubMed1994

Migration of Thy-1+ dendritic epidermal cells (Thy-1+DEC): Ly48 and TNF-alpha are responsible for the migration of Thy-1+DEC to the epidermis.

Thy-1+ dendritic epidermal cells (Thy-1+DEC) are mainly T cells that express T-cell receptor gamma and delta chains with limited diversity of gamma delta, mainly gamma 3 delta 1; such gamma 3 delta 1 TCR-bearing Thy-1+DEC originate from day 16 fetal thymic cells. To understand the migratory capability of Thy-1+DEC, we developed an in vitro model, using skin organ culture. First, emigration of Thy-1+DEC from the epidermis was examined. Ear skin from C3H/He mice was separated into two parts and incubated for 3 d with dermal side down. Thy-1+DEC emigrated from the epidermis into the dermis and then migrated out of the skin into the culture medium. Next, immigration of Thy-1+DEC into the epidermis was examined. Thy-1+DEC were depleted in vivo by daily application of clobetazole propionate solution topically onto the ears of C3H/He mice. Seven days later, ear skin was harvested, separated, and cultured with the dermal side up with syngeneic epidermal cell suspensions with a migration chamber for 3 d. It was found that 1) Thy-1+DEC immigrated into the Thy-1+DEC depleted epidermis as well as into untreated epidermis, and 2) the migratory capability of Thy-1+DEC was directly proved by a biolabeling technique with PKH-26. Blocking studies with various antibodies revealed that leukosialin (S11 monoclonal antibodies) and TNF alpha were important for Thy-1+DEC migration. Thus, Thy-1+DEC retain the potential for migration in vitro, and leukosialin and TNF alpha are partially responsible for the migration of Thy-1+DEC into the epidermis.

Animals↗
PubMed1994

Bilateral periorbital eccrine hidrocystoma.

We saw four patients showing identical features as cystic lesions on the bilateral external canthi. Histological examination showed cystic cavities in the dermis. Histological and enzyme histochemical findings suggest that these cystic tumors are of eccrine origin. Thus we diagnosed these cystic tumors as eccrine hidrocystoma with characteristic clinical feature. The recognition of this feature would help to correctly diagnose these eccrine hidrocystoma.

Adult↗
PubMed1994

Vogt-Koyanagi-Harada's syndrome associated with Hashimoto's thyroiditis.

Vogt-Koyanagi-Harada's syndrome has been known as an autoimmune disease which affects the melanocyte-containing tissues. We report a case of Vogt-Koyanagi-Harada's syndrome associated with Hashimoto's thyroiditis. IgM-class antibodies against nevus cells were detected in the patient's serum by indirect immunofluorescence test. These clinical and laboratory findings support the autoimmune theory of this rare syndrome.

Adult↗
PubMed1994

Interferon-gamma-induced HLA-DR, but not ICAM-1, expression of human keratinocytes is down-regulated by calmodulin antagonist.

Interferon-gamma (IFN-gamma) has been shown to induce or enhance the expression of MHC class II and intercellular adhesion molecule-1 (ICAM-1) in a variety of human and murine cell types, including epidermal keratinocytes (KC). However, the expression of MHC class II and ICAM-1 molecules induced by IFN-gamma is not necessarily coordinated. We investigated the inhibitory effects of the calmodulin antagonist, W-7, and its chlorine deficient inactive analogue, W-5, on the expression of MHC class II (HLA-DR) and ICAM-1 by human KC incubated with IFN-gamma. We found that the IFN-gamma-induced expression of HLA-DR was reproducibly and dose-dependently inhibited by W-7. However, the expression of ICAM-1 was highly resistant to the inhibitory effects of W-7. Neither HLA-DR nor ICAM-1 expression was affected by W-5. These data suggest that the IFN-gamma-induced HLA-DR, but not ICAM-1, expression is mediated, if not exclusively, by calmodulin in human KC.

Calmodulin↗
PubMed1994

Immunotherapy for Stewart-Treves syndrome. Usefulness of intrapleural administration of tumor-infiltrating lymphocytes against massive pleural effusion caused by metastatic angiosarcoma.

We describe a 56-year-old woman with Stewart-Treves syndrome who had severe dyspnea from a pleural effusion caused by metastatic angiosarcoma in the right lung. Tumor-infiltrating lymphocytes (TIL) in the pleural effusion were cultured and expanded in vitro in the continuous presence of recombinant interleukin 2 with periodic stimulation by CD3 antibody. The expanded TIL were administered intrapleurally seven times at 1- to 4-week intervals in combination with intravenous infusion of recombinant interleukin 2. A panel of T-cell clones was also obtained from TIL. Immunotherapy dramatically improved the patient's dyspnea and pleural effusion. A CD4+ T-cell clone and a CD8+ T-cell clone established from TIL had specific cytotoxicity to the tumor cells.

Breast Neoplasms↗
PubMed1993

Quantitative determination of human aldose reductase by enzyme-linked immunosorbent assay. Immunoassay of human aldose reductase.

An antibody-sandwich enzyme-linked immunosorbent assay (ELISA) for evaluating tissue levels of aldose reductase was developed using a polyclonal antibody prepared against the recombinant enzyme expressed in a baculovirus system. The specificity of this antibody to aldose reductase was verified by immunoprecipitation, immunoblotting and ELISA. The polyclonal antibody did not crossreact with human aldehyde reductase, an enzyme in the same aldo-keto reductase family structurally and functionally related to aldose reductase. The sensitivity and specificity of this assay method enabled direct determination of aldose reductase level in various human tissues including the erythrocyte. The highest level of aldose reductase was detected in the kidney medulla among tissues investigated. More than a 2-fold variability in the erythrocyte aldose reductase was demonstrated among healthy individuals, indicating the heterogeneity of this enzyme expression in a human population. This assay system may be useful for direct measurement of the level of tissue aldose reductase in conjunction with the evaluation of the efficacy of aldose reductase inhibitors prescribed for the treatment of diabetic complications.

Adult↗
PubMed1993

Amelanotic metastatic melanoma in a patient with oculocutaneous albinism.

Melanomas are rare in patients who have albinism, compared with the frequent occurrence of squamous cell carcinomas and basal cell carcinomas. This report describes amelanotic metastatic melanoma in a 58-year-old Japanese man who had tyrosinase-positive oculocutaneous albinism. A prolonged bleeding time, facioscapulohumeral muscular dystrophy, and Gilbert syndrome were also present. Superficial spreading melanoma with evidence of spontaneous regression on his right forearm was suspected as a possible primary site. Twenty-two cases of melanomas in persons who have albinism have been reported.

Albinism, Oculocutaneous↗
PubMed1993

[Skin disorders with eosinophilia].

Many inflammatory or neoplastic dermatologic disorders have been recognized to be associated with tissue and/or blood eosinophilia. Furthermore, the degree of local and accompanying blood eosinophilia usually reflect the intensity and extent of the disease activity in various skin disorders. Recent advancement in the biology of eosinophils have also clarified the pathogenetic role of eosinophils in some diseases. In this issue, the dermatologic disorders with eosinophilia are reviewed.

Blood Proteins↗
PubMed1992

Systemic elastolytic granulomatosis with cutaneous, ocular, lymph nodal, and intestinal involvement. Spectrum of annular elastolytic giant cell granuloma and sarcoidosis.

A 15-year-old Japanese girl had widespread annular serpiginous erythematous plaques, bilateral granulomatous uveitis, bloody diarrhea, and seronegative arthralgia. She also had anemia and leukopenia. The histopathologic findings were compatible with those of annular elastolytic giant cell granuloma. Elastolytic granulomas were also found in the cervical lymph nodes, terminal ileum, parietal peritoneum, and mesentery. Bilateral hilar lymphadenopathy, hypercalcemia, and an increased level of angiotensin converting enzyme were not observed throughout the clinical course. To the best of our knowledge, systemic elastolytic granulomatosis has not been previously described in annular elastolytic giant cell granuloma or sarcoidosis. This case may represent a type of granulomatosis in the broad spectrum of annular elastolytic giant cell granuloma and sarcoidosis.

Adolescent↗
PubMed1992

Epitopes for CD1a, CD1b, and CD1c antigens are differentially mapped on Langerhans cells, dermal dendritic cells, keratinocytes, and basement membrane zone in human skin.

BACKGROUND: CD1 antigens are classified serologically into at least three groups, CD1a, CD1b, and CD1c, and many kinds of monoclonal antibodies are available for each subgroup of CD1 antigens. CD1a, CD1b, and CD1c antigens have been shown to be selectively and differentially expressed on epidermal Langerhans cells and dermal dendritic cells in normal human skin. OBJECTIVE: The objective was to further delineate the localization of epitopes of CD1 antigens in human skin. METHODS: We examined the immunoreactivity of 14 different CD1 antibodies (seven CD1a, five CD1b, and two CD1c antibodies) with the immunoperoxidase technique. We also studied the reactivity of NU-T2 (CD1b) antibody by immunogold electron microscopy. RESULTS: The epitopes for CD1a, CD1b, and CD1c antigens were differentially mapped on epidermal Langerhans cells, dermal dendritic cells, keratinocytes, the luminal portion of eccrine gland ducts, and the basement membrane zone in human skin. CONCLUSION: These CD1 antibodies may be useful to analyze the phenotypic alteration of immune and nonimmune cells in various skin diseases.

Antigens, CD↗
PubMed1992

Production of IL-4, IL-2, IFN-gamma, and TNF-alpha by peripheral blood mononuclear cells of patients with atopic dermatitis.

Recently, T cell-derived cytokines have been postulated to be involved in the pathogenesis of atopic dermatitis (AD) since the synthesis of IgE is profoundly regulated by cytokines such as IL-4, IFN-gamma and IL-2. IL-4 enhances the production of IgE and in contrast, IFN-gamma inhibits this IL-4-mediated IgE production. IL-2 also prevents IL-4-induced production of IgE by a different mechanism from IFN-gamma, suggesting that the level of IgE is regulated by the quantitative balance of these antagonizing cytokines. We examined the production of IL-4, IL-2, IFN-gamma and TNF-alpha by PBMC of AD patients and non-AD controls. Although the production of IL-2 and TNF-alpha by AD patients was significantly lower than that of non-AD controls, the production of IL-4 and IFN-gamma did not show significant differences between AD and non-AD individuals. There was no significant correlation between cytokine production and clinical symptoms in AD patients. There was significant positive correlation between IL-4 and IL-2 production, and between IFN-gamma and TNF-alpha production. Serum IL-4 levels showed no significant difference between AD group and non-AD group.

Adolescent↗