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Biomedical subjects

M Fujioka

Publications and source records attributed to M Fujioka.

At least 271 records · Page 15Linked to original sources
PubMed1981

Formation of omega- and (omega-1)-hydroxyfatty acids and plausible formation of ketofatty acid from microsomal phospholipids.

Rat liver microsomes labeled with spin-labeled phosphatidylcholine release the label into the aqueous phase during the aerobic incubation with NADPH (Biochem. Biophys. Res. Commun. (1979) 87, 300-307). To establish the chemical nature of the released moiety, microsomes were labeled with [14C]phosphatidylcholine. When the 14C-labeled microsomes were incubated with NADPH under aerobic conditions, a few percent of the radioactivity was liberated into the aqueous phase within 60 min. Thin-layer chromatographic analysis of the radioactive substance liberated showed the presence of hydroxylated fatty acids derived from the 2-position of glycerol moiety. About one-third of the fatty acids formed from [14C]phosphatidylcholine during the incubation were converted into hydroxy-derivatives. Gas chromatography/mass spectrometry analysis further confirmed an NADPH-dependent formation of 16-hydroxypalmitic acid, 15-hydroxypalmitic acid, and hydroxy-derivatives of other fatty acids from the phospholipids of the microsomal membrane. Evidence was also obtained indicating the formation of ketopalmitic acid.

Animals↗
PubMed1981

Purification and characterization of cytosolic and mitochondrial serine hydroxymethyltransferases from rat liver.

The cytosolic and mitochondrial forms of serine hydroxymethyltransferase [EC 2.1.2.1] were purified to homogeneity from a whole homogenate of rat liver without the prior separation of mitochondria. The molecular weight of the cytosolic enzyme was 230,000, and that of the mitochondrial enzyme was 200,000. Each of the isozymes contained 4 mol of pyridoxal 5'-phosphate/mol. Tryptic peptide analyses of the NaBH4-reduced and carboxymethylated isozymes showed that each contained a single peptide containing phosphopyridoxyllysine. The numbers of peptides obtained were about one-fourth of those expected from their contents of lysine plus arginine residues. These findings together with the identity of subunit molecular weight indicate that each of the isozymes is composed of 4 identical polypeptide chains. The isoelectric pH values of the cytosolic and mitochondrial enzymes were 4.95 and 5.30, respectively. Other differences between the isozymes include the amino acid composition, stability of the apoenzyme, reactivity toward L-allothreonine, and immunochemical properties.

Amino Acids↗
PubMed1981

Cystic meconium peritonitis.

Meconium peritonitis occasionally occurs as a localized collection of meconium contained in a cyst made of fibrous granulation tissue. The cyst may contain only meconium or also encase loops of bowel. One such case is reported.

Cysts↗
PubMed1981

[Clinical evaluation of cardiac function test using scintiview (author's transl)].

Cardiac function was studied with Scintiview in 107 cases, 24 normal and 83 affected cases, and the following results were obtained. 1. Better data were obtained with converging collimeter than with parallel collimeter in cardiac study. (2) Red blood cells were sufficiently labeled with 99mTc using stannous chloride as reductant, and it was proven to be applicable to measurement of circulation blood volume. (3) Pulmonary circulation time and the systemic circulation time calculated from time activity curve, greater than 9.0 seconds and greater than 25 seconds, respectively, were considered abnormal prolongation. (4) Cardiac output index (cardiac output/circulation blood volume), less than 1.10 was considered decreased cardiac output. (5) Stroke volume index ((stroke volume/circulation blood volume)x 100, less than 1.70 was considered decreased stroke volume. (6) Ejection fraction, less than 60% was considered decreased left ventricular wall motion. (7) Cardiac function index (cardiac output index X ejection fraction), less than 80 was considered decreased cardiac performance.

Adolescent↗
PubMed1980

Amino acid sequence of a peptide containing an essential cysteine residue of yeast saccharopine dehydrogenase (L-lysine-forming).

The yeast saccharopine dehydrogenase (L-lysine-forming) contains an essential cysteine residue at the active site which can be carboxymethylated selectively by iodoacetate (Ogawa, H., Okamoto, M. and Fujioka, M. (1979) J. Biol. Chem. 254, 7030--7035). An undecapeptide containing this residue was isolated from the chymotryptic digest of the carboxymethylated enzyme by gel filtration chromatography and preparative paper electrophoresis. The amino acid sequence of the peptide was determined as Gly-Arg-Cys*-Gly-Ser-Gly-Ala-Leu-Ile-Asp-Leu, by the sequential Edman degradation and digestion with carboxypeptidases.

Amino Acid Sequence↗
PubMed1980

The inactivation of saccharopine dehydrogenase (L-lysine-forming) by diethyl pyrocarbonate.

Saccharopine dehydrogenase (epsilon-N-(L-glutaryl-2)-L-lysine: NAD oxidoreductase (L-lysine-forming) EC 1.5.1.7) from baker's yeast is inactivated by diethyl pyrocarbonate. Spectrophotometric studies show that the inactivation results from the modification of 3 histidyl residues/molecule of enzyme. The sulfhydryl content of the enzyme is unchanged by modification. The reversibility of inactivation by hydroxylamine and the pH dependence of inactivation are also consistent with the inactivation being due to modification of the histidyl residue. Although the coenzyme and substrates are without effect when added singly, the inactivation is completely protected by alpha-ketoglutarate in the presence of a saturating concentration of NADH. Since alpha-ketoglutarate binds only to the enzyme . NADH complex, the results suggest that the inactivation is due to modification of the residue at or near the substrate-binding site. Under the conditions where the inactivation is largely protected by NADH plus alpha-ketoglutarate, 2 histidyl residues appear to be modified suggesting that only 1 residue involved in the catalytic activity. The modification appears to prevent the binding of alpha-ketoglutarate, but not of the coenzyme, to the enzyme. The protein fluorescence of the native and modified enzymes is quenched by NAD+ and NADH. However, the NADH titration curve of the modified enzyme is not affected by alpha-ketoglutarate, in contrast to the native enzyme which shows an increase in the apparent affinity for the coenzyme in the presence of alpha-ketoglutarate.

Binding Sites↗
PubMed1980

Anorectal pressure and rectal compliance after low anterior resection.

Measurements of the resting pressure profile of the anorectum, recto-anal reflex and of rectal compliance (reservoir function of the rectum) were made in 24 normal subjects and 16 patients who had undergone low anterior resection for cancer of the sigmoid colon or upper half of the rectum. In the early postoperative period (< 6 month) frequent bowel movements and occasional soiling were experienced by most patients. Fifteen of 16 such patients had regular bowel movements and no soiling after six months. The present studies disclosed that there was a close correlation between control of bowel movements and rectal compliance, but not between the resting pressure profile of the anorectum or the recto-anal reflex and control of bowel movements after low anterior resection.

Adult↗
PubMed1980

Pseudoweb of the gastric antrum in infants.

Two infant boys, one 3 months of age and the other 8 weeks of age, with symptoms suggesting gastric outlet obstruction had upper gastrointestinal fluororadiographic examinations which showed typical signs for gastric antral webs (diaphragms or membranes), but neither had actual webs as proven by surgery in one and by endoscopy in the other. The report emphasizes the possibility of falsely positive radiographic findings, which can be tested by potentially corroborative fiberoptic endoscopy, thus avoiding unnecessary surgery.

Humans↗
PubMed1980

Polyarteritis nodosa in children: radiological aspects and diagnostic correlation.

The radiological findings in 14 children with polyarteritis nodosa (PN) were evaluated in relation to clinical, laboratory, and histological findings. In this series, there were two major groups of radiological findings, one associated with renal insufficiency and the other with intestinal vascular insufficiency. No radiographic finding was pathognomonic. Two patients were suspected of having small bowel intussusception (SBI), and SBI was documented at laparotomy in a third patient. The diagnosis and management of PN in children is dependent upon the correlation of clinical and laboratory findings. Associated pertinent radiological features which may be useful in the management of children with PN are reviewed.

Cardiomegaly↗