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Biomedical subjects

M Fujimaki

Publications and source records attributed to M Fujimaki.

At least 145 records · Page 8Linked to original sources
PubMed1983

Effect of metoclopramide on the secretion of aldosterone and other adrenocortical steroids.

The aim of this study was to determine the effect of metoclopramide, a dopamine antagonist, on the secretion of aldosterone and other adrenocortical steroids in normal subjects. An i.v. bolus injection of 10 mg of metoclopramide significantly increased the plasma PRL, plasma aldosterone and 18-hydroxycorticosterone, but the plasma renin activity, plasma deoxycorticosterone, corticosterone and cortisol remained unchanged. The changes in plasma aldosterone induced by metoclopramide were significantly correlated with the basal levels of plasma aldosterone and renin activity. These results suggest that the response of plasma aldosterone to metoclopramide in normal subjects is influenced by the basal activity of renin-angiotensin-aldosterone system and the late step of aldosterone synthesis is stimulated by metoclopramide.

18-Hydroxycorticosterone↗
PubMed1983

Mechanism of the central effects of dopamine and metoclopramide on aldosterone regulation in the rat.

To investigate the mechanism of the central action of dopamine and its antagonist, metoclopramide, on the regulation of aldosterone, studies were performed in 54 conscious rats with and without bilateral nephrectomy. In normal and sham-operated rats, intracerebroventricular injection of dopamine resulted in a significant suppression of plasma renin activity and plasma aldosterone at 30 min, and intracerebroventricular injection of metoclopramide resulted in a significant elevation of plasma renin activity and plasma aldosterone at 30 min without altering the plasma corticosterone and potassium levels. In bilaterally nephrectomized rats, the plasma renin activity was significantly reduced and it did not respond to dopamine or metoclopramide. In these rats, intracerebroventricular injection of metoclopramide exerted no effect on the plasma aldosterone, but intracerebroventricular injection of dopamine increased the plasma aldosterone slightly. However, this increase was not statistically significant. These findings suggest that the dopaminergic system in the brain is involved in the regulation of aldosterone secretion, mainly with changes in the peripheral renin-angiotensin axis in rats.

Adrenocorticotropic Hormone↗
PubMed1983

In vivo and in vitro effects of metoclopramide on aldosterone secretion in rats.

This study was designed to investigate the effects of metoclopramide, a dopamine antagonist, on in vivo and in vitro aldosterone production in the rat. In addition, we examined the effect of various levels of sodium intake on the response of plasma aldosterone to metoclopramide. Metoclopramide (2-50 mg/kg) was given by ip injection to conscious rats. Metoclopramide induced a dose-related increase in plasma aldosterone, whereas it only increased plasma renin activity at high doses (20 and 50 mg/kg). The response of plasma aldosterone to 10 mg/kg of metoclopramide in the low-sodium group was greater than that in the high-sodium group. Metoclopramide had no effect on aldosterone production in isolated zona glomerulosa cells in vitro.

Adrenal Glands↗
PubMed1983

Prolactin, renin and catecholamines in essential hypertension.

In 40 male patients with essential hypertension less than the age of 36 years, the plasma prolactin (PRL), plasma renin activity (PRA) and plasma catecholamines (noradrenaline (NA) and adrenaline (A)) were determined simultaneously. Those levels were significantly increased compared to the levels in 14 age-matched healthy male control subjects. Furthermore, there were significant correlations between the PRL and plasma NA or PRA (p less than 0.05 in each case), and between the PRA and plasma NA (p less than 0.05). However, no significant relationships were observed between systolic or diastolic blood pressure and PRL, plasma NA or PRA. These findings indicate that the central dopaminergic activity is reduced in a number of young adults with essential hypertension, and that in the patients with the reduced central dopaminergic activity, the peripheral sympathetic activity is stimulated and PRA is also increased probably due to the increased peripheral sympathetic activity, and the possibility exists that such changes are related to the development of hypertension.

Adult↗
PubMed1983

Lipid composition of winged bean (Psophocarpus tetragonolobus).

The lipids were extracted from the winged bean (Psophocarpus tetragonolobus) seed with water-saturated n-butanol. Lipids were separated into groups by preparative TLC on silica gel G. The amount of each lipid type was determined by analysis of the fatty acid constituents in each lipid type. Glyceride was the major lipid accounting for 89.6% of the total, followed by an unknown lipid 4%, free fatty acid of 2.3%, 1,3-diglyceride, 1,2-diglyceride and steryl ester as 1% each and finally a polar lipid as 0.2%. The results show that winged bean oil should be suitable for edible purposes. Triglycerides showed a similar profile of fatty acids to those of whole lipid: the major fatty acids were palmitic (10.9%), stearic (4.5%), oleic (37.1%), linoleic (19.0%), eicosenoic (3.6%), behenic (18.5%) and lignoceric (4.2%) acids. Compared to soybean oil, winged bean oil contained long chain fatty acids and a fairly small amount of linolenic acid which is favorable regarding oil stability against autoxidation.

Chemical Phenomena↗
PubMed1983

[Case report of adenoma of the duodenal papilla].

A 67-year-old woman was hospitalized with fever and jaundice. She immediately received PTCD because of obstructive jaundice. Hypotonic duodenography and endoscopic examination revealed a protuberance with an irregular surface at the site corresponding to the papilla Vateri. No evidence of malignancy was obtained from biopsied specimens of the lesion. Pancreatoduodenectomy was successfully performed. Histological examination of the resected specimen disclosed villous adenoma containing adenocarcinoma. The problems of histopathogenesis of cancer in adenoma arising in this particular region are discussed and the necessity of pancreatoduodenectomy in this particular neoplasma is emphasized.

Adenoma↗
PubMed1982

Evaluation of the renin-angiotensin system in diabetes insipidus and psychogenic polydipsia.

The role of the renin-angiotensin system in polyuric patients was studied in 5 patients with pituitary diabetes insipidus, 1 patient with nephrogenic diabetes insipidus and 3 patients with psychogenic polydipsia by determining the plasma renin activity and infusing an angiotensin II analog, 1-Sar, 8-Ile angiotensin II. In all patients with diabetes insipidus, plasma renin activity was markedly increased and the blood pressure was reduced in 5 of 6 patients by the administration of the angiotensin II analog. In the other patient, the blood pressure remained unchanged. The plasma renin activity and response of blood pressure to the angiotensin II analog in a patient with nephrogenic diabetes insipidus were not significantly different from there of patients with pituitary diabetes insipidus. On the other hand, in all patients with psychogenic polydipsia, plasma renin activity was normal or low, and the blood pressure increased with the administration of the angiotensin II analog. These results suggest that evaluation of the renin-angiotensin system by determining plasma renin activity and infusing an angiotensin II analog is useful in differentiating between diabetes insipidus and psychogenic polydipsia.

1-Sarcosine-8-Isoleucine Angiotensin II↗
PubMed1982

[Phase II study on peplomycin for esophageal cancer].

For the purpose of verifying the effectiveness of peplomycin, one of the derivatives of bleomycin, against carcinoma of the esophagus and the safety of it, the analysis of the data for total 113 cases collected from 25 institutions in Japan was made. The results are as follows. It was effective in 19 out of 74 evaluable cases of carcinoma of the esophagus (25.7%). In case of treatment with peplomycin alone, it was effective in 6 out of 39 cases (15.4%). In case of the combination treatment with peplomycin and some other therapy, it was effective in 13 out of 35 cases (37.1%). As for the side effects, the incidence of fever was the highest in both the cases of peplomycin alone and the combination treatment such as 39.6% and 37.0%, respectively. Anorexia, nausea, vomiting, respiratory symptoms and tiredness were found in relatively many cases. In the clinical laboratory tests, the vital capacity after the treatment tended to be lower than that before the treatment, but there was little change in the hematological tests, pulmonary function test and renal function test.

Adult↗
PubMed1981

Central and peripheral effects of dopamine on the renin-angiotensin-aldosterone system in conscious rats.

To investigate the role of the central and peripheral dopaminergic systems in the control of the renin-angiotensin-aldosterone system in conscious rats, dopamine or its antagonist, metoclopramide, was injected intravenously (i.v.) and intracerebroventricularly (i.c.v.). Dopamine (100 micrograms/kg), when injected i.c.v., decreased plasma renin activity (PRA) and plasma aldosterone concentration (PA), while metoclopramide (50 micrograms/kg, i.c.v.) increased both of them. Intravenous administration of dopamine (1 microgram/kg/min) did not produce significant changes in either PRA or PA. In contrast, metoclopramide (500 micrograms/kg, i.v.) increased PA, which was not accompanied by any change in PRA. Blood pressure was decreased by i.c.v. administration of dopamine and increased by i.c.v. injection of metoclopramide, whereas no change in blood pressure was observed when these compounds were administered i.v. Dopamine and metoclopramide, injected i.v. or i.c.v., did not produce significant changes in plasma sodium, potassium and corticosterone concentrations. These results suggest that the dopaminergic system in the brain regulates renin secretion, thereby changing PA. In contrast, dopamine receptors of the adrenal glands may inhibit aldosterone secretion, which is not mediated by changes in the renin-angiotensin system, plasma potassium and ACTH.

Aldosterone↗
PubMed1980

Purification and some properties of a myofibrillar protein-degrading protease, cathepsin L, from rabbit skeletal muscle.

A newly-identified muscle protease which degraded myosin, alpha-actinin, and actin, and removed the Ca-sensitivity of the ATPase activity of myofibrils was purified from rabbit skeletal muscle by ammonium sulfate fractionation, Sephadex G-75 chromatography, P-cellulose chromatography, Sephadex G-75 rechromatography, and Ultrogel AcA 54 chromatography. Polyacrylamide gel electrophoresis showed that the enzyme thus obtained was almost homogeneous. The molecular weight of the enzyme was found to be 24,000 by gel filtration on Sephadex G-75. The optimum pH for the Ca-sensitivity-removing activity was pH 7.0, and that for the activity to degrade myosin was pH 4.1. The enzyme was stable at pH 4.5--6.5. It was strongly inhibited by iodoacetate, leupeptin, and antipain, but not by pepstatin or phenylmethane sulfonyl fluoride. EDTA was essential for the activity of the enzyme. The enzyme did not split alpha-N-benzoyl-DL-arginine-beta-naphthylamide. Since these properties resemble those of cathepsin L isolated from rat liver lysosomes, the enzyme was regarded as muscle cathepsin L.

Animals↗