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Biomedical subjects

M Fujii

Publications and source records attributed to M Fujii.

At least 595 records · Page 33Linked to original sources
PubMed1989

Study on digestibility and energy availability of daily food intake by the Japanese. Part 2. Horse mackerel and milk.

Four male Japanese students were fed a semisynthetic diet including rice and whole egg as protein for seven days (basal-diet period), and in the following seven days milk and then, for seven days, horse mackerel were added at the expense of part of the corn starch and sugar in the basal diet (test-diet period). Urine and feces were collected throughout all periods and the contents of protein, fat and energy in these extra were determined. The results obtained were as follows: 1) Digestibility of protein was 97.3 +/- 4.1% for milk and 96.8 +/- 2.8% for horse mackerel. 2) Digestibility of fat was 96.7 +/- 1.9% for milk and 98.6 +/- 2.6% for horse mackerel. 3) The ratio of the total available energy to intake energy (Net Energy Availability) was 92.5 +/- 2.9% for milk and 88.4 +/- 5.2% for horse mackerel.

Adult↗
PubMed1989

Interposition of a saphenous vein graft for early recurrent carotid stenosis--report of two cases.

The main cause of recurrent stenosis within 24 months of carotid endarterectomy is myointimal hyperplasia. In these cases, reoperation is technically difficult because there is no true plane of dissection between the recurrent plaque and the remaining vessel wall. The authors present two cases of early recurrent stenosis after carotid endarterectomy, both of which were successfully treated by an interposed saphenous vein graft. In the first case, angiography suggested that the recurrent stenosis was due to atherosclerosis. In the second case, however, myointimal hyperplasia was suspected to be the cause, and this was confirmed by histological examination of the surgical specimen. The authors' experience indicates that anastomosis with an interposed saphenous vein graft is a reliably effective treatment for early recurrent carotid stenosis.

Arterial Occlusive Diseases↗
PubMed1989

Tumor growth-promoting effect of immunosuppressive substance in mice.

The effect of immunosuppressive (IS) substance obtained from cancerous ascitic fluid on tumor growth and host immunity in plasmacytoma X5563-bearing C3H/He mice is described. IS substance given in three injections, before and after tumor inoculation caused: (a) enhanced tumor growth, (b) marked reduction in survival times, (c) inhibition on Con-A response of spleen cells. Depressed natural killer (NK) activity was observed in normal and tumor-bearing mice treated with IS substance. The data presented here suggest that IS substance suppresses both humoral and cellular immunoresponsiveness and tumor cells evade immune surveillance or immunologically mediated removal.

Animals↗
PubMed1989

[An autopsied case of pulmonary blastoma with high serum alpha-fetoprotein and carcinoembryonic antigen levels].

A 75-year-old male case of a pulmonary blastoma with high serum alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) levels is reported. A mass shadow in the right lower lung field was visible on examination of a chest X-ray. Serum AFP and CEA levels were 466.1 ng/ml and 5.8 ng/ml, respectively. The autopsy revealed a pulmonary blastoma composed of malignant epithelial and mesenchymal elements. In the epithelial elements, AFP and CEA producing tumor cells were detected by immunoenzymatic labelling (PAP) methods.

Aged↗
PubMed1989

[Inhibitory effect of a new proglumide derivative, loxiglumide, on CCK action in isolated rat pancreatic acini].

The effect of a new proglumide derivative, loxiglumide (DL-4-(3,4-dichloro-benzoyl-amino)-5-(N-3-methoxy-propyl-pentylamino+ ++)-5-oxo-pentanic acid; CR 1505), on binding of 125I-CCK-8 and amylase release stimulated by CCK-8 was investigated in isolated rat pancreatic acini. Loxiglumide inhibited CCK-8-stimulated amylase release and binding of 125I-CCK-8 to rat pancreatic acini in a dose-dependent manner. Loxiglumide caused a concentration-dependent rightward shift of the dose-response curve for CCK-8-stimulated amylase release without altering the maximal response. Schild plots showed a slope of 0.82 and pA2 value of 7.05. The inhibitory effect of loxiglumide on amylase release was reversible. Loxiglumide significantly inhibited amylase release in response to CCK-8, caerulein and gastrin-I. However, loxiglumide had no effect on amylase release stimulated by other receptor secretagogues (bombesin, carbamylcholine, secretion and vasoactive intestinal polypeptide) or by agents bypassing receptors (A23187 and TPA). These results indicate that loxiglumide acts as a potent, competitive and specific CCK antagonist on the pancreatic acini.

Amylases↗
PubMed1989

Bioassay of plasma cholecystokinin in rat and human: inhibition of protein synthesis prevents the decrease in the sensitivity and responsiveness of isolated rat pancreatic acini to CCK-8.

Isolated rat pancreatic acini were most sensitive and responsive when stimulated directly with cholecystokinin octapeptide (CCK-8) without preincubation. Both the responsiveness and sensitivity of acini to CCK-8 decreased time dependently with prolonged preincubation. When acini were stimulated with CCK-8 following pulse labeling with radioactive leucine, old protein was discharged together with newly synthesized (labeled) protein. However, the sensitivity and responsiveness of these acini for release of labeled protein were primarily reduced with preincubation, indicating that newly synthesized protein was contributing to the time-dependent loss of sensitivity and responsiveness. Then isolated acini were treated with 300 microM cycloheximide for 2 h. This treatment prevented the decreases in the sensitivity and responsiveness of amylase release to CCK-8 stimulation and made these alterations in one series of experiments negligible. Using these acini, a sensitive and specific bioassay for the measurement of CCK in human and rat plasma was developed.

Amylases↗
PubMed1989

[Plasma cholecystokinin concentration in patients with chronic pancreatitis measured by bioassay].

We developed a specific and sensitive bioassay for measuring plasma cholecystokinin (CCK) in human and investigated CCK response after a test meal in patients with chronic pancreatitis. Treatment with cycloheximide increased the sensitivity and responsiveness of isolated rat pancreatic acini to CCK-octapeptide (CCK-8) and thus plasma levels of CCK-8 as low was 0.17 pM were detectable. Fasting plasma CCK levels in normal subjects as CCK-8 equivalents were 0.75 +/- 0.25 pM and rose to a peak of 6.2 +/- 0.68 pM at 45 min after a test meal consisting of 400 ml milk and 2 boiled eggs. Basal and stimulated plasma levels of CCK-8 in patients with non-calcified chronic pancreatitis were significantly higher than those in normal subjects. In contrast, postprandial responses of plasma CCK-8 in patients with calcified chronic pancreatitis was significantly low compared to those in control subjects, although basal plasma levels were not significantly different from those in controls.

Amylases↗
PubMed1989

[Successful treatment of advanced hypopharyngeal cancer with combination chemotherapy (VPCP regimen) followed by subsequent radiotherapy: a case report].

This report concerns a 64-year-old male suffering from advanced hypopharyngeal cancer. This patient was treated with four courses of combination chemotherapy including VPCP (a combination of VCR, PEP, CDDP and PEP) regimen as neo-adjuvant chemotherapy. And radiotherapy was given as a secondary treatment; Linac. 60 Gy/6 weeks. He showed no tumor masses in the hypopharynx following this combination chemotherapy. No recurrence has been found under endoscopy for one year after the treatment with combination chemotherapy. It thus seems that neo-adjuvant chemotherapy prior to surgery and/or radiation including cisplatin, peplomycin and other agents is very useful as a multimodal treatment for cancer of the hypopharynx.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1989

[Chemosensitivity of human head and neck tumors detected by human tumor clonogenic assay].

Chemosensitivity of human head and neck tumor was evaluated by human tumor clonogenic assay. Among eight of head and neck tumors seven squamous cell carcinomas such as tongue, gingiva, maxillary sinus, pharynx and one malignant fibrous histiocytoma (MFH) of maxillary sinus and four anti-cancer agents BLM, CDDP, 5-FU and MMC were used. Five of eight tumors were succeeded to evaluate the sensitivity. Positive rates except MFH were BLM 100% (3/3), CDDP 25% (1/4), 5-FU 33% (1/3). These results are similar to recent clinical experiments except CDDP.

Bleomycin↗
PubMed1989

[Radio- and radio-chemosensitivity of human head and neck cancer cell line detected by human tumor clonogenic assay].

The radiosensitivity and radio-chemosensitivity of 3 series of human cancer cell lines were evaluated by human tumor clonogenic assay. The sources of cell lines were gingiva carcinoma (Ca9-22), uterus carcinoma (Hela) and gastric carcinoma (MKN-45). BLM and CDDP were used, and chemosensitivity of gingiva carcinoma tended to be higher than other cell lines. Radiosensitivity was same as MKN-45. Isobologram were employed for quantitation of the interaction between the irradiation and anti-cancer agents. In Ca9-22, the interaction of between gamma-rays, BLM and CDDP was supra-additive. Hela was also supra-additive, but in MKN-45, the interaction of between gamma-rays and BLM was sub-additive.

Bleomycin↗
PubMed1989

[Effect of fluoride releasing composite resin on bovine enamel].

FluorEver is one kind of fluoride releasing composite resin. The first aim of this study was to investigate the amount of fluoride released from FluorEver. The second aim of this study was to determine the uptake of fluoride with bovine enamel, which was immersed in distilled water for 28 days in contact with FluorEver, compared with Fuji Ionomer cement which released fluoride. The results obtained were as follows. 1) A great amount of fluoride was released from FluorEver immediately after immersion. Twenty-four hours after immersion, the amount of released fluoride decreased quickly. From the 3rd day of immersion, the amount of fluoride released decreased gradually. The fluoride could be detected on the 28th day. 2) The amount of fluoride released from FluorEver was almost directly proportional to the volume of FluorEver blocks used. 3) The uptake of fluoride on enamel in contact with FluorEver was significantly high. 4) The release pattern of the fluoride of Fuji Ionomer was similar to that of FluorEver. The amount of fluoride released from Fuji Ionomer was greater than that of FluorEver immediately after immersion. However, from the 7th day of immersion, that of Fuji Ionomer was less than that of FluorEver. 5) The uptake of fluoride from FluorEver on the surface enamel was less than that of Fuji Ionomer. However, the uptake of fluoride from FluorEver on the subsurface enamel was greater than that of Fuji Ionomer.

Animals↗
PubMed1989

[Availability of intermittent chemotherapy by reservoir in liver metastasis of colo-rectal cancer].

From August 1986 to May 1989, in 18 cases of liver metastasis from colo-rectal cancer, intermittent chemotherapy was undergone by implantation reservoir in the subcutaneous. Intra-arterial infusion chemotherapy was under taken in 6 cases, and intra-portal vein chemotherapy in 12 cases. 5-FU 500 mg + MMC 10-6 mg was infused through implantable reservoir once every two weeks. CDDP and ADM were also infused in one case. Infusions averaged 12.4 times, and the most was 24 times in the group of intra-arterial infusion chemotherapy. In the group of intra-portal vein infusion chemotherapy, infusion averaged 12.4 times, with the highest at 35 times. In liver metastasis (H3), there is no significant difference between intra-arterial infusion chemotherapy and intra-portal vein infusion chemotherapy, revealing the cumulative survival rate. And the reservoir group compares with the 14 cases of hepatectomy and the 9 cases of systemic chemotherapy in cumulative survival rate. As a result, there was no significant difference between the group with the reservoir and the systemic chemotherapy group in H1 cases and H2 cases, revealing a median survival rate in these treatment groups. The median survival of reservoir group was 8.3 months, and that of the hepatectomy group was 20.6 months in H2 cases. In the H3 cases, the median survival of the reservoir group was 9.6 months, and that of the systemic chemotherapy group was 10.3 months. Reservoir was implanted in three cases to prevent recurrence after hepatectomy. As a result, two of three cases have survived for 20 months and 10 months, respectively.

Adult↗
PubMed1989

[Measurement of carcinoembryonic antigen, squamous cell carcinoma related antigen and neuron-specific enolase in the bronchoalveolar lavage fluid in patients with peripheral lung cancer].

Carcinoembryonic antigen (CEA), squamous cell carcinoma related antigen (SCC) and neuron-specific enolase (NSE) in bronchoalveolar lavage fluid were measured in 30 patients with peripheral lung cancer, 11 patients with benign lung disease and 19 healthy controls. The mean levels and positive rates of lavaged fluid CEA were 128.0 +/- 16.9 ng/mg and 33.3% in patients with lung cancer, 68.1 +/- 25.9 ng/mg and 9.1% in patients with benign lung disease, and 68.3 +/- 11.6 ng/mg and 5.2% in healthy controls, respectively. The mean levels and positive rates of lavaged fluid CEA in patients with lung cancer were significantly higher than those in patients with benign lung disease (p less than 0.05) and those in healthy controls (p less than 0.05). The mean levels of lavaged fluid SCC and NSE showed no significant difference between cases of lung cancer and benign lung disease or healthy controls. No lavaged tumor marker level in patients with lung cancer showed any close correlation with histologic types and serum levels. In conclusion, measurement of lavaged fluid CEA was considered to be useful in the differential diagnosis of peripheral lung cancer.

Adenocarcinoma↗
PubMed1989

Effects of vitamin C long-term loading on the serum gamma-glutamyltranspeptidase activity in human subjects.

In an attempt to assess dietary effects on the serum enzyme activity of long-term vitamin C supplementation, the serum gamma-GTP(1) fraction was studied in five healthy men. In this study, they were maintained on ordinary meals for the initial one year and the last 7 months of the 43 month experimental period, and on ordinary meals with 1 g/day of vitamin C powder for the intermediate 2 years. The serum gamma-GTP(1) fraction during the first year (ordinary meals alone) was higher than that for the second or third year (ordinary meals with addition of vitamin C). The decrease after vitamin C supplementation was statistically significant. The serum vitamin C concentration tended to rise in winter. Vitamin C supplementation at 1 g/day raised the concentrations up to 1.6 mg/dl on average, where the serum was practically saturated with vitamin C.

Adult↗