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Biomedical subjects

M Fu

Publications and source records attributed to M Fu.

At least 109 records · Page 6Linked to original sources

Intracardiac echocardiography via the transvenous approach with use of 8F 10-MHz ultrasound catheters.

OBJECTIVE: To study the safety and feasibility of intracardiac imaging using a novel 8F 10-MHz non-over-the-wire ultrasound catheter system. SUBJECTS AND METHOD: Intracardiac imaging using a transfemoral venous approach was performed in 33 adults, 14 men and 19 women, aged 25 to 66 years (mean, 46 years). Six were normal subjects, 12 had congenital heart diseases (5 atrial septal defects, 3 ventricular septal defects, 1 tetralogy of Fallot, 2 patent ductus arteriosus, and 1 Ebstein anomaly), 14 had valvular heart diseases (12 mitral stenoses and 2 calcific aortic stenoses), and 1 had acute pulmonary embolism. RESULTS: Ultrasound images were obtained, without any complications, from the right side of the heart in all subjects. The atria and ventricles could be recognized by anatomic relationships to the cardiac chambers and the valves by their characteristic motion during each cardiac cycle. The vessels were verified by their connections to the cardiac chamber and by contrast echocardiography if indicated. CONCLUSION: Intracardiac imaging using the 8F 10-MHz non-over-the-wire ultrasound catheter system via a transfemoral venous approach is feasible and safe. Intracardiac echocardiography is potentially useful for assessing a variety of cardiac anomalies and in guiding and monitoring certain intervention procedures.

Adult↗

Coronary dissection and myocardial infarction following blunt chest trauma.

Myocardial infarction (MI) following blunt chest trauma is rarely diagnosed because the ensuing cardiac pain is commonly attributed to contused myocardium or the traumatic injuries in the local chest wall. There are only scattered reports on the coronary pathology associated with MI secondary to blunt chest trauma. Because differentiation of the pathology is difficult but important, we report here three cases of acute anterior MI secondary to coronary dissection following blunt chest trauma. Coronary dissection was demonstrated by coronary angiography. Two of the patients had intimal tears at the proximal left anterior descending artery (LAD) with normal flow, and the other patient had nearly total occlusion of the LAD associated with filling defects probably caused by an intracoronary thrombus. All three patients received conservative treatment without major complications and remained free from angina or heart failure throughout a 5-year follow-up period. In order to exclude associated MI in cases of blunt chest trauma, electrocardiography is necessary, and coronary angiography may be indicated to demonstrate coronary arterial pathology. Dissection of the coronary artery with subsequent thrombus formation is one of the possible pathophysiologic mechanisms of MI following blunt chest trauma.

Adult↗

Macrophage TNF mRNA expression induced by LPS is regulated by sphingomyelin metabolites.

Metabolism of macrophage (MO) membrane phospholipids produces key mediators of inflammation and major second messengers that modulate inflammatory responses during sepsis. Sphingomyelin is a major class of phospholipid that releases ceramide and sphingosine. This study was designed to investigate the involvement of sphingomyelin metabolites in MO activation by lipopolysaccharide (LPS). Rabbit alveolar MO were obtained by bronchoalveolar lavage and exposed to C6-ceramide, a cell-permeable analogue of natural ceramide, or sphingosine in the presence of Escherichia coli LPS (100 ng/mL). Tumor necrosis factor (TNF) mRNA expression was measured by Northern blot assays. Total nuclear extract was harvested for the measurement of nuclear factor KB (NFkappaB) with electrophoretic mobility shift assays. MO TNF production was measured by L929 bioassays. C-6 ceramide did not have any effects on MO TNF production or TNF mRNA expression with or without LPS stimulation. Inhibition of ceramide metabolism with 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), or N-oleoyl-ethanolamine (NOE) also did not induce TNF mRNA or TNF production. In comparison, sphingosine inhibited TNF mRNA expression as well as TNF production of LPS-stimulated MO. LPS-induced MO NFkappaB activity was also reduced by sphingosine. Our data indicate that ceramide alone has no effect on macrophage activity, but its metabolite sphingosine down-regulates MO activation induced by LPS stimulation. Therefore, the sphingomyelin pathway is involved in the regulation of MO activation.

Animals↗

Primary stenting of the left main coronary artery in acute myocardial infarction complicated by cardiogenic shock: case report.

Although acute left main coronary artery (LMCA) occlusion is a rare angiographic finding, it carries a very high mortality rate and most of the patients with this clinical condition die from sudden death or cardiogenic shock due to malignant arrhythmia or pump failure. The high mortality rate and tendency to lead rapidly to death are chiefly related to extensive myocardial injury. We report a case of cardiogenic shock resulting from acute myocardial infarction. Emergency cardiac catheterization was performed and coronary angiography showed a totally occluded LMCA. Prompt revascularization by means of primary LMCA stenting was successful and the patient was discharged 2 weeks later with only mild congestive heart failure. We suggest that the rapid performance of angiographic studies in this patient with cardiogenic shock was the turning point in saving her life. We also suggest primary LMCA stenting as an effective procedure for saving lives because it may reverse cardiogenic shock and prevent a probable fatal outcome.

Aged↗

[Effect of human plasma HDL on the HDL receptors of plasma membranes of cholesterol-fed rabbit].

Atherosclerosis(As) rabbit model was developed by high-cholesterol feeding for 12 weeks. The rabbits were injected intravenously with human plasma HDL preparation per week, and then the effects of HDL on the lipids contents of serum, liver and bile nd the activity of HDL receptors on liver plasma membranes of cholesterol-fed rabbit were investigated. The results showed that HDL preparation had no effect on decreasing the lipids contents of serum, but it could low down the lipid depositions in liver, and promote the excretion of lipids from bile. The value of Bmax of HDL receptor showed decreasing trendy and the value of Kd showed increasing trendy in cholesterol-fed rabbits. In HDL-treated rabbits, the value of Bmax increased significantly as compared with that of normal group(P < 0.05), but the value of Kd showed no difference. The results suggested that human plasma HDL could enhance the activity of HDL receptors on the liver plasma membranes of cholesterol-fed rabbit.

Animals↗

Lack of human papillomavirus types 16 and 18 DNA in esophageal squamous carcinoma cell lines.

OBJECTIVE: To assess the role of human papillomavirus (HPV) in the carcinogenesis of the esophageal carcinomas in a high-incidence geographical area in Northern China. METHODS: The presence of HPV types 16 and 18 DNA in the three esophageal carcinoma cell lines was examined by type-specific polymerase chain reaction (PCR). RESULTS: No HPV type 16 and 18 DNA was found in any of the three esophageal carcinoma cell lines. CONCLUSION: The results suggest that the role of HPV in human esophageal carcinogenesis in a high-incidence geographical area remains to be elucidated.

Adult↗

[A study on the conjugation of Streptococcus mutans adhesins and its salivary receptors].

OBJECTIVE: To investigate the conjugation specificity of Streptococcus mutans' adhesins and their salivary receptors. METHODS: Include purified salivary receptor or adhesin competitive bacteria adhesion inhibition test and enzyme linked immuno-receptor assay (ELIRA) on S. mutans WD9463A. RESULTS: Salivary amylases can both enhance and competitively inhibit the adhesion of the strain to HA, while IgA degraded fragments and MW = 13,000 protein only promote its adhesion. P1 and a 117,000 surface protein of WD9463A both inhibit adhesion of the strain to IgA degraded fragments and salivary amylases. An 127,000 protein of WD9463A and a kind of GTFase inhibit its adhesion to IgA degraded fragments and salivary amylases respectively. No conjugation was found between IgA degraded fragments and the adhesins, while reaction between amylases and the adhesins had basically accordance with adhesion inhibition test. CONCLUSION: The adhesion of S. mutans is a result of reaction between multireceptors and multiadhesins.

Adhesins, Bacterial↗

[Magnetic resonance imaging in dental appliance treatment of obstructive sleep apnea syndrome].

OBJECTIVE: To investigate the upper airway changes after wearing dental appliance in obstructive sleep apnea syndrome(OSAS). METHODS: The research included 11 aged OSAS patients and 11 matched controls. Through dental appliance therapy the OSAS was proved by polysomnography to have ideal treatment results (AHI had decreased from 44.6 +/- 22.5 per hour to 7.53 +/- 6.44 per hour). Magnetic resonance imaging (MRI) had been performed before and after wearing dental appliance. The MRI images of upper airway after wearing dental appliance were compared with those of without wearing, and even with those of the controls. RESULTS: After wearing dental appliance the size of upper airway increased greatly, from 12.27 ml +/- 4.13 ml to 13.93 ml +/- 4.58 ml(P < 0.01), While the size of oropharynx was increased from 5.55 ml +/- 2.10 ml to 6.88 ml +/- 2.26 ml (P < 0.001). There was still remained significant difference at nasopharynx, soft palate and tongue compared with the matched controls. CONCLUSION: Dental appliance worked with the effect of enlarging upper airway. The abnormal airway morphology can not be changed completely by dental appliance treatment. MRI technique, especially the 3-D reconstruction model of upper airway, is an effective and special method in OSAS research.

Adult↗

[Pre- and post surgical orthodontic treatment of mandibular prognathism].

OBJECTIVE: To analyze the patients we treated in clinic through combined orthodontic-orthognathic surgery, and get some guidance for the future clinical work. METHODS: All 40 skeletal Class III malocclusion patients we analyzed were from joint clinic of our hospital. They aged from 17 to 38. RESULTS: The duration for presurgical orthodontic treatment was 9 months on average (2-25 months), and for postsurgical orthodontic treatment about 7.6 months on average (2-15 months). The duration for whole active treatment was 16 months on average (4-41 months). The objectives of presurgical orthodontic are: Alignment of dental arches, decompensation of anterior and posterior teeth, leveling of arches, and coordination of the width of the upper and lower arches. After presurgical orthodontic treatment, most of the patients could be treated by one piece surgery, which could simplify the surgical procedure and reduce the relapse rate of surgery. During the surgery, we modified the intermaxilary fixation wires. We used rigid rectangular stainless steel wires with Edgewise appliances as the fixation wires instead of conventional canine and molar bands with multiloop wires. The aims of the postsurgical orthodontic treatment are: closing residual spacing, extrusion of posterior teeth for correction of local openbite in premolars region, paralleling of the teeth, and adjustment of occlusion. CONCLUSION: Pre- and post-ssurgical orthodontic treatment is essential to the surgical treatment for mandibular prognathism.

Adolescent↗

[19 cases of Paget's disease].

OBJECTIVE: The clinical signs of Paget's disease are similar to those of chronic eczema. The two are often confused, especially extramammary Paget's disease. Only biopsy can help to differentiate them correctly. METHODS: 19 patients with Paget's disease were reviewed with regard to clinical analysis and treatment from 1987 to 1997. RESULTS: Six patients had breast Paget's disease and 13 had extramammary one. The latter involved scrotum and penis in 11 patients, the groin in 1, and perianal and anal in 1. Follow up showed that 3 patients had recurrences in extramammary patients who had had positive surgical margin biopsy. One patient died of other disease. CONCLUSIONS: Surgery is the first choice of treatment for Paget's disease either of breast or of extramammary regions. Negative margins must be achieved to prevent local recurrence.

Adult↗

[Study on the contents of serum HDL subclasses in type N hyperlipidemics].

Contents of serum HDL subclasses in normolipidemics (n = 24) and type IV hyperlipidemics (n = 24) were determined using two-dimensional gel electrophoresis associated with immunodetection method. The results showed that the contents of pre-beta 1 HDL (P < 0.001) and HDL3a (P < 0.05) increased significantly and that of HDL2b (P < 0.001) decreased significantly in hyperlipidemics, compared with normolipidemics. Serum TG concentrations of hyperlipidemics showed positive correlation with pre-beta 1 HDL (r = 0.582) and HDL3a (r = 0.692) and negative correlation with HDL2b (r = -0.506) and HDL2a (r = -0.552). These results indicated that the maturation of HDL could be abnormal in type IV hyperlipidemics.

Adult↗

[Separation and selection of salivary adhesion receptors of Streptococcus mutans to tooth surface].

OBJECTIVE: To select and purify salivary receptors of Streptococcus mutans from experimental salivary acquired pellicle. METHODS: Experimental salivary acquired pellicle (SAP) was performed by coating hydroxyapatite (HA) with whole saliva. Then SAP was washed from HA by 1 mol/L NaCl and 0.5 mol/L phosphate buffer sequentially. The proteins were further separated by chromatography of Sephadex G75 and DEAE-Sephadex A25. Receptors of Streptococcus mutans were selected by bacterial adhesion test and competitive inhibition adhesion test. Identification was performed by PAGE, SDS-PAGE, IP-PAGE and detection of amylase activity and inmunodifusion test. RESULTS: IgA degraded fragments, a protein of 13 kD and amylases were the receptors of S. mutans. The first two only promote the adhesion but the amylases can both promote and inhibit S. mutans adhesion. CONCLUSION: The adhesion of S. mutans to tooth surface is a result of interaction between adhesins of S. mutans and multiple salivary receptors.

Adhesins, Bacterial↗

[Study on relative factors in endoscopic sinus surgical procedure].

OBJECTIVE: In order to investigate the factors which affect the operative treatment effect of FESS. METHOD: 215 cases suffered functional endoscopic sinus surgery (FESS) from May 1994 to December 1997 were analysed. RESULT: The clinical curative rate was 75.8% and the improving rate was 96.3%. CONCLUSION: The effect shows that surgical procedure is closely related to following factors: mechanic of operation, drugs taken in pre-operation, kinds of anesthsia, clinical stage of chronic sinusitis, nasal poplys and skill of surgeons.

Adolescent↗

[Nasopharygeal size in obstructive sleep apnea syndrome].

OBJECTIVE: To investigate the impact of upper airway 3-D size on the obstructive sleep apnea syndrome (OSAS). METHODS: There were 20 male and 3 female OSAS patients involved in the research. The measured apnea-hypopnea index (AHI) was 45.72(24.01 episodes per hour of sleep). The items measured from magnetic resonance imageing (MRI) of upper airway size were compared to those from 12 normal controls that were sex and age matched to the OSAS patients. The Pearson correlation and multiple linear regression had done to investigate into the relationship of AHI and MRI data. RESULTS: The OSAS patients had relatively narrow nasopharynges than matched controls in the following items: the sagittal size, transverse size, section area and volume. The measured sagittal size of the nasopharynx was closely related to AHI and Lown SaO2, and the volume of nasopharynx was related to apnea index (AI). CONCLUSION: The nasopharyngeal size is closely associated with OSAS.

Adult↗

Middle cerebral artery occlusion after recent Mycoplasma pneumoniae infection.

Stroke is a rare complication of Mycoplasma pneumoniae infection although recent studies suggest that infection may be a risk factor of acute ischemic stroke. We report a 5-year-old girl who had a stroke 10 days after M. pneumoniae infection. This is the first case of documented middle cerebral artery occlusion after Mycoplasma infection in children. In this article, we review other published reports on this topic and discuss the possible underlying mechanisms of the middle cerebral artery occlusion.

Arterial Occlusive Diseases↗

Prostaglandin I2 analogue, iloprost, down regulates mitogen-activated protein kinases of macrophages.

OBJECTIVE: Vascular endothelial cells (EC) play a pivotal role in diffuse organ injury seen in ARDS and MOFS. On exposure to cytokines or endotoxin (LPS) EC are stimulated to express adhesion molecules as well as proinflammatory and procoagulant activity. However, the potential feedback control of EC on macrophages (M-theta) is not clear. We studied the cellular mechanism of iloprost, a PGI2 analogue, in regulation of TNF production by LPS-stimulated M-theta. METHODS: Rabbit alveolar M-theta and mouse M-theta RAW 264.7 cells were exposed to Escherichia coli LPS in the presence of various concentrations of iloprost. TNF production was measured by L929 bioassays. To further study the cellular mechanism of iloprost on M-theta activation, RAW 264.7 cells were stimulated by LPS (10 micrograms/ml) in the presence of either iloprost or specific mitogen-activated protein kinase (MAPK) inhibitors, either PD98059 or SB202190. P44/P42 and P38 MAPK activation were evaluated by Western blot assays with anti-phospho MAPK antibodies. RESULTS: LPS induced M-theta TNF production, which was inhibited by iloprost. Iloprost also attenuated the activation of P44/P42 and P38 induced LPS. Inhibition of P44/P42 with PD98059 or P38 with SB202190 significantly reduced TNF production by LPS-stimulated RAW cells. CONCLUSIONS: The regulatory mechanism of EC on M-theta activation is dependent on PGI2. The effect of PGI2 on M-theta is, at least in part, mediated through inhibiting MAPKs.

Animals↗

Interleukin 10 inhibits alveolar macrophage production of inflammatory mediators involved in adult respiratory distress syndrome.

BACKGROUND: Adult respiratory distress syndrome (ARDS) causes severe morbidity and mortality in trauma patients. One potential method to attenuate the lung injury is to inhibit alveolar macrophage production of proinflammatory mediators. The purpose of this study was to investigate the cellular mechanism of interleukin 10 (IL-10) inhibition on LPS-stimulated macrophage (Mphi). We hypothesized that IL-10 inhibited phospholipase C signal pathways in Mphi. IL-10 inhibition would be restored by calcium ionophores and protein kinase C (PKC) activation. METHODS: Rabbit alveolar Mphi were obtained by bronchoalveolar lavage. Mphi were treated with Escherichia coli LPS (10 ng/ml) in the presence of various concentrations of human IL-10. Cell lysates and supernatant were analyzed for proagulants (PCA) and tumor necrosis factor (TNF), respectively. TNF mRNA expression of alveolar Mphi was also measured by Northern Blot assay. Macrophage PGE2 production was measured by ELISA. RESULTS: IL-10 inhibited the production of both TNF and PCA by LPS-stimulated Mphi. In addition, IL-10 also reduced TNF mRNA expression. Similarly, PGE2 production by LPS-stimulated Mphi was also attenuated by IL-10. An increase in the intracellular [Ca2+] induced by A23187 failed to reverse this IL-10-mediated inhibition. In comparison, phorbol myristate acetate, a protein kinase C (PKC) activator, restored TNF and PCA production despite the presence of IL-10. CONCLUSIONS: IL-10 inhibits Mphi production of inflammatory mediators. This inhibition is, at least in part, mediated by modulating the PKC activity.

Animals↗

Radiofrequency and cryoablation of atrial fibrillation in patients undergoing valvular operations.

BACKGROUND: Previous studies have shown that the maze operation can restore sinus rhythm and atrial transport function in patients with chronic atrial fibrillation. The purpose of this study was to test the feasibility of the application of radiofrequency and cryoablation as an alternative to the classic maze operation. METHODS: Twelve patients undergoing mitral valve procedures were included in this study. Radiofrequency and cryoablation were applied to create lesions in both atria to simulate the classic maze operation. RESULTS: There were two surgical deaths. At the mean follow-up of 10.25 months for the remaining 10 patients; 6 were in sinus rhythm, 2 in atrial rhythm, 1 in paroxysmal atrial tachycardia, and 1 in atrial fibrillation. Doppler echocardiography at 6-month follow-up showed emergence of biatrial transport function in 3 patients and right atrial contractility in 8. At 12-month follow-up of 5 patients, Doppler echocardiography showed biatrial transport function in 3 and right atrial contractility in 4. CONCLUSIONS: Our modified maze procedure during valvular operation is effective for achieving an acceptable success rate to restore sinus rhythm and atrial transport function in patients with chronic atrial fibrillation.

Adult↗