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Biomedical subjects

M Friedlander

Publications and source records attributed to M Friedlander.

32 records · Page 2Linked to original sources
PubMed1987

Combination chemotherapy followed by surgery or radiotherapy in patients with locally advanced cervical cancer.

Forty-seven patients with locally advanced cervical cancer at high risk of relapse received three cycles of chemotherapy with PVB (cisplatin, vinblastine and bleomycin) before definitive local treatment with either radical surgery or radiotherapy. Thirty-one of the 47 patients (66%) responded to initial chemotherapy, and 11 of them have relapsed compared with 13 of the 16 non-responders. Median time to recurrence was 31 weeks for PVB non-responders but has not yet been reached for PVB responders. After a median follow-up of 128 weeks, 14 of the 31 responders (45%) are alive and disease free compared with 3 of the 16 non-responders (19%). There was a positive correlation between response to chemotherapy and subsequent response to radiotherapy. PVB was in general well tolerated although one death is probably attributable to chemotherapy. A randomized study comparing radiotherapy alone with initial PVB chemotherapy followed by radiotherapy is in progress.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1987

Magnetic resonance imaging in choroidal tumors.

Magnetic resonance imaging (MRI) was performed on 13 patients with choroidal tumors to determine whether this technique could detect and portray an intraocular tumor and to investigate additional biochemical parameters of the diseased tissue. In 11 of 13 patients, the lesion was depicted, and its gross morphology was outlined with a clarity approaching that of computed tomography. We were able to apply principles derived from MRI studies in other portions of the body and identify trends in the characterization of lesions of different etiologies. The relative T1 and T2 values for each of the major pathologic categories (malignant melanoma, metastatic carcinoma, and benign choroidal lesions) are presented and discussed. In addition, MRI was clearly superior to other techniques in the detection and delineation of associated intracranial lesions.

Choroid Neoplasms↗
PubMed1985

Glycogen metabolism and the nuclear envelope-annulate lamella system in the early chick embryo.

The intracellular sites of glycogen degradation in the mid to late uterine chick embryo were determined by cytochemical localization of glucose-6-phosphatase at the ultrastructural level. Enzyme activity was found between the two membranes of the nuclear envelope, in the annulate lamellae and in specialized glycogen-containing membrane scrolls. Annulate lamellae and glycogen scrolls were most frequent during the stages of intensive glycogen degradation. Annulate lamellae appear to be formed from the nuclear envelope. During the early post-laying stages annulate lamellae disappeared and were replaced by endoplasmic reticulum that appeared initially in scroll-like formations.

Animals↗
PubMed1979

Immunological studies of the embryonic muscle cell surface. Antiserum to the prefusion myoblast.

Xenogeneic antisera raised in rabbits have been used to detect compositional changes at the cell surfaces of differentiating embryonic chick skeletal muscle. In this report, we present the serological characterization of antiserum (Anti-M-24) against muscle tissue and developmental stage-specific cell surface antigens of the prefusion myoblast. Cells from primary cultures of 12-d-old embryonic chick hindlimb muscle were injected into rabbits, and the resulting antisera were selectively absorbed to obtain immunological specificity. Cytotoxicity and immunohistochemical assays were used to test this antiserum. Absorption with embryonic or adult chick heart, brain, retina, liver, erythrocytes, or skeletal muscle fibroblasts failed to remove all reactivity of Anti-M-24 for myogenic cells at all stages of development. After absorption with embryonic myotubes, however, Anti-M-24 no longer reacted with differentiated myofibers, but did react with prefusion myoblasts. The myoblast surface antigens detected with Anti-M-24 are components of the muscle cell membrane: (a) these macromolecules are free to diffuse laterally within the myoblast membrane; (b) Anti-M-24, in the presence of complement, induced lysis of the muscle cell membrane; and (c) intact monolayers of viable myoblasts completely absorbed reactivity of Anti-M-24 for myoblasts. These antigens are not loosely adsorbed culture medium components or an artifact of tissue culture because: (a) absorption of Anti-M-24 with homogenized embryonic muscle removed all antibodies to cultured myoblasts; (b) Anti-M-24 reacted with myoblast surfaces in vivo; and (c) absorption of Anti-M-24 with culture media did not affect the titer of this antiserum for myoblasts. We conclude that myogenic cells at all stages of development possess externally exposed antigens which are undetected on other embryonic and adult chick tissues. In addition, myoblasts exhibit surface antigenic determinants that are either masked, absent, or present in very low concentrations on skeletal muscle fibroblasts, embryonic myotubes, or adult myofibers. These antigens are free to diffuse laterally within the myoblast membrane and may be modulated in response to appropriate environmental cues during myodifferentiation.

Animals↗
PubMed1977

Surface antigens of the embryonic chick myoblast: expression on freshly trypsinized cells.

Using an antiserum raised in rabbits against embryonic chick skeletal myoblasts (Anti-M-24), we have examined the trypsin and neuraminidase sensitivity and physiological expression of myoenic cell surface antigens. It was found that trypsin-released muscle cells more effectively inhibited, on a cell to cell basis, the cytotoxicity of Anti-M-24 for 24-h-old myoblast monolayers than did identical cells that had received a 3-4 h suspension culture recovery period from trypsinization. There was no such difference in absorptive capacities observed for any other embryonic chick tissue tested (e.g. brain, retina, liver, heart, and red blood cells) when freshly trypsinized cells were compared to ones which were given a 3-4 h culture period. If freshly trypsinized muscle cells were treated with high concentrations (30,000 international units (IU)/0.1 ml packed cells) of trypsin or with neuraminidase (30,000 IU/ml packed cells), there was a selective loss of myoblast-specific surface antigens. When single cells that had been in suspension culture for 3.5 h were reexposed to low concentrations (10,000 IU/0.1 ml packed cells) of trypsin, more antigenic sites were revealed on their surfaces as detected by an increased absorptive capacity in removing myoblast-binding antibodies from Anti-M-24. This increase in antigenic expression was time-dependent and inversely related to the length of culture time after trypsinization. Immunofluorescence studies revealed that tissue specific myoblast cell surface antigens are present on both muscle cells that were freshly dissociated and those that had been in suspension culture for 3-4 h. Furthermore, freshly trypsinized myoblasts possessed cell surface components that were highly antigenic; antiserum to such cells reacted extensively with both trypsinized and recovered muscle cells as detected by complement-dependent 51Cr release cytotoxicity assays and immunofluorescence. We conclude that embryonic chick myoblasts possess surface antigens that may be selectively removed by neuraminidase or high concentrations of trypsin. These antigens may be progressively masked, with increasing time of culture after protease-dissociation, by molecules that are sensitive to low concentrations of trypsin. Such masking of tissue-specific cell surface antigens could result in the display of molecular mosaics which may play a role in facilitating intercellular recognition and subsequent differentiation and histogenesis.

Animals↗
PubMed1977

Cryptococcosis.

Explore the source record for details and available documents.

Adult↗
PubMedDate not supplied

Bovine opsin has more than one signal sequence.

By deletion of selected segments from a bovine opsin complementary DNA clone and subsequent analysis of transcripts in a cell-free translation-translocation system, we have localized two out of four theoretically conceivable signal sequences required for the integration of opsin into microsomal membranes.

Amino Acid Sequence↗