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Biomedical subjects

M Fischer

Publications and source records attributed to M Fischer.

At least 325 records · Page 18Linked to original sources
PubMed1996

Anti-GM1 antibodies in polyneuropathies of unknown origin.

This study was undertaken to determine whether anti-GM1 titres are raised in polyneuropathies of unknown origin and whether determination of these titres is useful for diagnosing these conditions. The study population comprised 20 controls (aged 36-88 years), 12 patients with polyneuropathies of known origin (aged 31-81 years) and 15 patients with polyneuropathies of unknown origin (aged 40-77 years). Antibody levels were measured using a commercial GM1 enzyme linked immunosorbent assay kit (Buehlmann Laboratories). Mean anti-GM1 IgG and IgM antibody titres were not raised in patients with polyneuropathies of unknown origin. Anti-GM1 IgG antibody titres were raised in one and GM1 IgM antibody titres in none of the patients with polyneuropathies of unknown origin. In conclusion, GM1 antibody levels are rarely raised in polyneuropathies of unknown origin and probably play a minor role in the pathogenesis of these conditions.

Adult↗
PubMed1996

Flow velocity of single lymphatic capillaries in human skin.

The purpose of this study was to investigate the previously unknown flow velocity in single lymphatic capillaries of humans in the supine position. Fifteen healthy subjects (10 women and 5 men; mean age 35.8 +/- 13.1 yr) were studied. Ten microliters of fluorescein isothiocyanate-dextran (150,000 mol wt) were injected into the subepidermal layer of the foot dorsum. The filling of the microlymphatics from the resulting depot was visualized by fluorescence video microscopy and stored on videotape. Flow velocity in the microlymphatics was determined on the video screen by direct measurement of the advancement of dyed lymph during a given time. The following median velocities were obtained: 0.51 mm/s (0.27 and 0.61 mm/s for lower and upper quartiles, respectively) for velocity during initial network filling and 9.7 microns/s (6.9 and 14.2 microns/s for lower and upper quartiles, respectively) for resting velocity at the end of the filling period. Mean lymphatic capillary diameter was 54.8 +/- 8.2 microns, and mean network extension was 8.3 +/- 3.2 mm. The high filling velocities are probably due to increased interstitial pressure and volume caused by dye microinjection, whereas the values measured during the end of network filling seem to approach resting flow velocities.

Adult↗
PubMed1996

Size influences on primate locomotion and body shape, with special emphasis on the locomotion of 'small mammals'.

The mechanical laws which make possible several characteristic and well-known modes of primate locomotion are reviewed. Biological requirements are fulfilled in small and in large primates by utilizing different mechanical principles. On the basis of the mechanics, special morphological traits can be identified which are advantageous for performing these locomotor modes, and which determine different life-styles. These morphological 'adaptations' consequently are different in larger and smaller primates. The divergence between large and small forms is clarified by the inclusion of non-primate mammals into the comparisons.

Animals↗
PubMed1996

Determination of language dominance using functional MRI: a comparison with the Wada test.

We performed functional MRI (FMRI) in 22 consecutive epilepsy patients undergoing intracarotid amobarbital (Wada) testing and compared language lateralization measures obtained with the two procedures. FMRI used a single-word semantic decision task previously shown to activate lateralized language areas in normal adults. Correlation between the two tests was highly significant (r = 0.96; 95% CIs 0.90 to 0.98; p < 0.0001). These results validate the FMRI technique and suggest that "active" areas observed with this semantic processing task correspond to those underlying hemispheric dominance for language. This strong correlation observed supports the view that language lateralization is a continuous rather than a dichotomous variable. In addition to lateralization information, FMRI consistently demonstrated focal regions of activity in lateral frontal and temporo-parieto-occipital cortex. These functional maps may be helpful in defining the boundaries of surgical excisions.

Adolescent↗
PubMed1996

Autologous bone transplant after sternal resection.

Two patients underwent subtotal sternal resection for tumors of the sternum. Anatomical and functional reconstruction was performed with bone fragments harvested from the internal lamina of both iliac wings. There were no infections and no instabilities. Late postoperative follow-up included CT-scan of the thorax and pulmonary function testing, which was not compromised. We conclude that this surgical approach is very efficient in regard to function and chest wall stability.

Bone Transplantation↗
PubMed1996

Cerebral resuscitation from cardiac arrest: pathophysiologic mechanisms.

Both the period of total circulatory arrest to the brain and postischemic-anoxic encephalopathy (cerebral postresuscitation syndrome or disease), after normothermic cardiac arrests of between 5 and 20 mins (no-flow), contribute to complex physiologic and chemical derangements. The best documented derangements include the delayed protracted inhomogeneous cerebral hypoperfusion (despite controlled normotension), excitotoxicity as an explanation for selectively vulnerable brain regions and neurons, and free radical-triggered chemical cascades to lipid peroxidation of membranes. Protracted hypoxemia without cardiac arrest (e.g., very high altitude) can cause angiogenesis; the trigger of it, which lyses basement membranes, might be a factor in post-cardiac arrest encephalopathy. Questions to be explored include: What are the changes and effects on outcome of neurotransmitters (other than glutamate), of catecholamines, of vascular changes (microinfarcts seen after asphyxia), osmotic gradients, free-radical reactions, DNA cleavage, and transient extracerebral organ malfunction? For future mechanism-oriented studies of the brain after cardiac arrest and innovative cardiopulmonary-cerebral resuscitation, increasingly reproducible outcome models of temporary global brain ischemia in rats and dogs are now available. Disagreements exist between experienced investigative groups on the most informative method for quantitative evaluation of morphologic brain damage. There is agreement on the desirability of using not only functional deficit and chemical changes, but also morphologic damage as end points.

Altitude Sickness↗
PubMed1996

Developmentally imprinted genes as markers for bladder tumor progression.

PURPOSE: Developmentally imprinted genes, such as H19 and insulin-like growth factor-II (IGF-II), play an important role during human embryogenesis and also have been implicated in the pathogenesis of embryonal tumors of childhood. Since H19 is expressed in human fetal bladder, we evaluated 35 bladder carcinomas for H19 expression by in situ hybridization analysis and correlated expression with tumor grade. As a prelude to gene transfer studies to determine if H19 is a bladder tumor oncogene, we also evaluated bladder cell lines for expression of H19, IGF-II, IGF-I and the type I IGF receptor. MATERIALS AND METHODS: H19 expression was evaluated by in situ hybridization analysis in bladder tumor specimens. Northern analysis was used to evaluate the expression of H19, IGF-II, IGF-I and the type I IGF receptor in bladder cell lines. RESULTS: H19 was expressed preferentially in advanced stage tumors: 2 of 12 grade I tumors were H19 positive, whereas 9 of 11 grade II and 7 of 10 grade III tumors expressed H19 (p = 0.004). Additionally, 6 of 6 carcinoma in situ tumors were H19 positive, whereas normal bladder mucosa cells were H19 negative. We found that 3 of 11 cell lines (HT-1376, HT-1197 and 5637) express high levels of H19 mRNA, and each of these cell lines and J82 also express IGF-II. All cell lines examined expressed the type I IGF receptor, whereas there was no detectable IGF-I mRNA. CONCLUSIONS: These data demonstrate that H19 is an oncodevelopmental marker of bladder tumor progression and raise the possibility that H19 may have oncogenic properties in bladder cancer.

Biomarkers, Tumor↗
PubMed1996

Early migration predicts late aseptic failure of hip sockets.

We report a prospective, stratified study of 60 PCA-cups and 60 RM-polyethylene cups which have been followed for a median time of 90 months, with annual radiography. The radiological migration of cups was measured by the computer-assisted EBRA method. A number of threshold migration rates from 1 mm in the first year to 1 mm in five years have been assessed and related to clinically determined revision rates. A total of 28 cups showed a total migration of 1 mm or more within the first two years; 13 of these cups have required revision and been exchanged. The survival curves of cups which had previously shown early migration were considerably different from those without early migration. For cups with a migration of less than 1 mm within the first two years the mean survival at 96 months was 0.96 +/- 0.02; for migrating cups, it was 0.63 +/- 0.11 (log-rank test, p=0.0001; chi-square value=39.4). Early migration is a good predictor for late loosening of hip sockets.

Aged↗
PubMed1996

[Pathogen spectrum and antibiotic resistance in peritonsillar abscess].

The study presented updates the spectrum of causative organisms in peritonsillar abscesses. Antibiotic treatment and surgery play important roles in this potentially life-threatening disease. A regular screening of the bacterial spectrum present and resistance remains important for optimizing a therapeutic regimen. Swabs of 122 peritonsillar abscesses were taken and investigated for the presence of causative organisms (bacteria and fungi) and potential antibiotic resistances. It was found that group A beta-hemolytic streptococci and Bacteroides spp. were the predominant isolates. It was also found that amoxicillin plus clavulinic acid or, alternatively, a third-generation cephalosporin (e.g., cefotaxime) represented the treatment of first choice to prevent antibiotic resistance.

Adolescent↗
PubMed1996

The value of arthroscopy in the evaluation of carpal instability.

In a prospective study, arthroscopic examinations were performed on 42 wrists following injury. Clinical findings, radiology, and arthrography were compared to the arthroscopic findings. Only obvious or total ligamentous ruptures found on arthroscopy were considered in the analysis. No direct correlation could be demonstrated with clinical findings or arthrography. The distribution pattern of ligament injury involving the radial and ulnar compartment of the wrist joint in 40% of the cases suggests that carpal dysfunction following injury and ligamentous tears is a more complex problem than usually accepted. The ulnar midcarpal joint seems more prone to injury than the radiocarpal portion of the wrist. This suggests an important biomechanical function of the palmar ligaments inserting on the triquetrum.

Adolescent↗
PubMed1996

[Carpal ligament lesions associated with fresh distal radius fractures: arthroscopic study of 54 cases].

AIM: Intracarpal ligamentous tears and fractures of the distal radius may have a similar injury mechanism. We performed arthroscopy of the wrist in 54 patients with distal radius fracture to find out the frequency of associated ligamentous tears. RESULTS: 41% of the patients with an extraarticular fracture of the distal radius had at least one severe ligamentous tear of a carpal ligament. In the group of patients with intraarticular fracture we detected ligamentous tears even in 89%. Most frequent were tears of the lunotriquetral ligament. DISCUSSION: Associated ligamentous tears are more common than expected. These tears may be of importance in the outcome of treatment of distal radius fractures.

Adolescent↗
PubMed1996

[Comparison of standard methods for the preparation of egg yolk antibodies].

Chicken egg yolk antibodies (lgY) play an increasing role as alternative to mammalian polyclonal antibodies. They are widely used in biomedical research, for diagnostics, prophylaxis, and therapy of diseases. The extraction steps of IgY from egg yolk must be simple, with high output of purified antibodies. The aim of the present study was a comparison of different purification methods of egg yolk antibodies. The results of eight extraction methods of IgY and method combinations were investigated by PAGE and densitometric analysis. It has been demonstrated, that the IgY preparation with dextran sulfate is very effective, quick and simple to perform. It is well-suited in combination with other methods, e.g. ammonium sulfate precipitation.

Animals↗
PubMed1996

[Rhythmic lymph extravasation from a lymph fistula].

A 66-year-old woman with a chronic traumatic lymph fistula is described. Using the almost atraumatic fluorescence micro-lymphography the fistula could be precisely localized within the ulcer. Lymph flow from deep channels was rhythmic. This previously unknown phenomenon of rhythmic retrograde flow in a lymphatic fistula is probably due to the activity of contractile segments of lymph collectors and an important factor for the non-healing of ulcers.

Aged↗
PubMed1995

Active site topology and reaction mechanism of GTP cyclohydrolase I.

GTP cyclohydrolase I of Escherichia coli is a torus-shaped homodecamer with D5 symmetry and catalyzes a complex ring expansion reaction conducive to the formation of dihydroneopterin triphosphate from GTP. The x-ray structure of a complex of the enzyme with the substrate analog, dGTP, bound at the active site was determined at a resolution of 3 A. In the decamer, 10 equivalent active sites are present, each of which contains a 10-A deep pocket formed by surface areas of 3 adjacent subunits. The substrate forms a complex hydrogen bond network with the protein. Active site residues were modified by site-directed mutagenesis, and enzyme activities of the mutant proteins were measured. On this basis, a mechanism of the enzyme-catalyzed reaction is proposed. Cleavage of the imidazole ring is initiated by protonation of N7 by His-179 followed by the attack of water at C8 of the purine system. Cystine Cys-110 Cys-181 may be involved in this reaction step. Opening of the imidazole ring may be in concert with cleavage of the furanose ring to generate a Schiff's base from the glycoside. The gamma-phosphate of GTP may be involved in the subsequent Amadori rearrangement of the carbohydrate side chain by activating the hydroxyl group of Ser-135.

Amino Acid Sequence↗