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Biomedical subjects

M Fink

Publications and source records attributed to M Fink.

At least 19 recordsLinked to original sources
PubMed2006

Gastrointestinal helminths in indigenous and exotic chickens in Vietnam: association of the intensity of infection with the Major Histocompatibility Complex.

This study compared the prevalence and intensity of infections of helminths in 2 chicken breeds in Vietnam, the indigenous Ri and the exotic Luong Phuong. Also, possible correlations with the Major Histocompatibility Complex (MHC) were tested. The most prevalent helminths were Ascaridia galli, Heterakis beramporia, Tetrameres mothedai, Capillaria obsignata, Raillietina echinobothrida and Raillietina tetragona. Differences in prevalence and intensity of infection were found between the 2 breeds. Comparing the 2 groups of adult birds, Ri chickens were observed to have higher prevalence and infection intensities of several species of helminths, as well as a higher mean number of helminth species. In contrast, A. galli and C. obsignata were shown to be more prevalent in Luong Phuong chickens. Furthermore, an age-dependent difference was indicated in the group of Ri chickens in which the prevalence and the intensity of infection was higher for the adult than the young chickens for most helminths. The most notable exception was the significantly lower prevalence and intensities of A. galli in the group of adult chickens. In contrast, the prevalence and intensity were very similar in both age groups of Luong Phuong chickens. Using a genetic marker located in the MHC, a statistically significant correlation between several MHC haplotypes and the infection intensity of different helminth species was inferred. This is the first report of an association of MHC haplotype with the intensity of parasite infections in chickens.

Animals↗
PubMed2006

[Above elbow amputation due to acupuncture treatment?].

Opinions of acupuncture experts should always be sought in any lawsuit or claim for compensation involving sequelae of acupuncture treatment. Overall,--in the hands of experienced acupuncturists--acupuncture is very safe but not without minor adverse events. However, serious adverse events are extremely rare, especially the risk of a serious vascular lesion. In contrast, inflammatory bowel disease is an independent and disease specific risk factor for thromboembolism, especially if the disease is highly active.

Acupuncture Therapy↗
PubMed2006

Time reversal of ultrasound through a phononic crystal.

In this Letter, we experimentally investigate time reversal focusing through a phononic crystal consisting of a periodic square arrangement of steel rods in water. An acoustic pulse is transmitted through the medium, received at a transducer array, time reversed and backpropagated. Both spatial focusing and time compression are studied and compared with those obtained through an equivalent disordered medium. With the phononic crystal, we do not observe the "hyperfocusing effect" that is typical of time reversal through disordered samples.

Journal Article↗
PubMed2006

Phosphodiesterase (PDE) 7 in inflammatory cells from patients with asthma and COPD.

In inflammatory cells, the low K(m) cyclic adenosine monophosphate (cAMP)-specific phosphodiesterase (PDE) 4 subtype is predominant in terms of expression and function, although more recently it has been suggested that PDE 7 may also play a role in regulating inflammatory cell activity. In the present study, PDE 4 and PDE 7 subtype messenger ribonucleic acid (mRNA) transcripts in CD4 and CD8 lymphocytes from healthy (n=10) and asthmatic (n=10) subjects and polymorphonuclear neutrophils (PMNs) and CD8 lymphocytes obtained from healthy (n=10) and chronic obstructive pulmonary disease (COPD) (n=7) subjects were identified and quantified. PDE 4A, PDE 4B, PDE 4D and PDE 7A mRNA were present in similar quantities in both CD4 and CD8 lymphocytes obtained from healthy and asthmatic subjects and in CD8 lymphocytes obtained from healthy and COPD subjects. Expression of PDE 4C and PDE 7B mRNA was also observed, although transcript levels were low and variable between individuals. In addition, the effects of selective PDE 7 inhibition on both phytohaemagluttinin (PHA)-induced human peripheral blood mixed mononuclear cell (HPBMNC) proliferation and fMLP-induced neutrophil elastase (NE) release were studied. HPBMNC and human neutrophils, isolated from the venous blood of healthy volunteers (n=6) were treated with either a novel selective PDE 7 inhibitor PF 0332040 alone or in combination with rolipram. Proliferation of HPBMNC was stimulated by PHA (2microgml(-1)) and assessed by [(3)H]-thymidine incorporation, while fMLP-induced (100nM) NE release was determined using a chromogenic substrate. Both rolipram (0.003-10microM) and PF 0332040 (0.003-10microM) significantly inhibited PHA-stimulated proliferation of HPBMNC ((**)P<0.01). Co-administration of rolipram (0.3-10microM) and PF 0332040 (0.003-10microM) significantly increased the degree of inhibition observed, compared to when either drug was administered alone ((**)P<0.01). PF 0332040 (0.003-10microM) had no inhibitory effect on NE release from human peripheral blood neutrophils stimulated with fMLP (100nM), while rolipram (0.003-10microM) significantly inhibited neutrophil degranulation ((**)P<0.01). These findings suggest no evidence of altered PDE 4 or PDE 7 mRNA transcript levels in inflammatory cells isolated from the peripheral venous blood of mild asymptomatic asthmatic subjects or stable COPD subjects, however, inhibition of PDE 7 may influence mononuclear cell function.

3',5'-Cyclic-AMP Phosphodiesterases↗
PubMed2006

Stimulus-dependent processing of temporal order.

Two distinct conceptualisations of processing mechanisms have been proposed in the research on the perception of temporal order, one that assumes a central-timing mechanism that is involved in the detection of temporal order independent of modality and stimulus type, another one assuming feature-specific mechanisms that are dependent on stimulus properties. In the present study, four different temporal-order judgement tasks were compared to test these two conceptualisations, that is, to determine whether common processes underlie temporal-order thresholds over different modalities and stimulus types or whether distinct processes are related to each task. Measurements varied regarding modality (visual and auditory) and stimulus properties (auditory modality: clicks and tones; visual modality: colour and position). Results indicate that the click and the tone paradigm, as well as the colour and position paradigm, correlate with each other. Besides these intra-modal relationships, cross-modal correlations show dependencies between the click, the colour and the position tasks. Both processing mechanisms seem to influence the detection of temporal order. While two different tones are integrated and processed by a more independent, possibly feature-specific mechanism, a more central, modality-independent timing mechanism contributes to the click, colour and position condition.

Acoustic Stimulation↗
PubMed2006

Adaptive focusing for transcranial ultrasound imaging using dual arrays.

Ultrasonic brain imaging remains difficult and limited because of the strong aberrating effects of the skull (absorption, diffusion and refraction of ultrasounds): high resolution transcranial imaging would require adaptive focusing techniques in order to correct the defocusing effect of the skull. In this paper, a noninvasive brain imaging device is presented. It is made of two identical linear arrays of 128 transducers located on each side of the skull. It is possible to separate the respective influence of the two bone windows on the path of an ultrasonic wave propagating from one array to the other, and thus estimate at each frequency the attenuation and phase shift locally induced by each bone window. The information obtained on attenuation and phase is used to correct the wave fronts that have to be sent through the skull in order to obtain a good focusing inside the skull. Compared to uncorrected wave fronts, the spatial shift of the focal spot is corrected, the width of the focal spot is reduced, and the sidelobes level is decreased up to 17 dB. Transcranial images of a phantom are presented and exhibit the improvement in image quality provided by this new noninvasive adaptive focusing method.

Adult↗
PubMed2005

Combinations of genetic changes in the human cAMP-responsive element modulator gene: a clue towards understanding some forms of male infertility?

The cAMP-responsive element modulator (CREM) gene plays a pivotal role in the mouse spermatogenesis, but its role in the human infertility has not been fully established. We performed a mutation screening in 13 Slovenian men with round spermatid arrest and in six controls. Eleven genetic changes have been identified in the human CREM gene, three novel single-nucleotide polymorphisms [within the promoters P1, P3 and intervening sequence 1 (IVS1)], one insertion (IVS2) and one non-sense mutation (exon gamma). Some infertile patients seem to accumulate potentially harmful genetic changes. We identified a patient with no CREM immunoreactive protein that was homozygous for the nucleotide changes in all promoters, IVS 1, 2, 6, and was heterozygous for the mutation in exon gamma. Interestingly, insertion in IVS2 (IVS2-58_55insT) results in a four-fold decrease in binding of nuclear proteins. Computer predictions suggested the presence of a potential novel CREM promoter, however, random amplification of cDNA ends from the human testis cDNA library was not successful in confirming a novel transcription start site of the CREM gene. Screening of a larger number of patients and controls is required to elucidate whether the observed combinations of genetic changes in the CREM gene can explain some forms of male infertility.

Adult↗
PubMed2005

Imaging anisotropic and viscous properties of breast tissue by magnetic resonance-elastography.

MR-elastography is a new technique for assessing the viscoelastic properties of tissue. One current focus of elastography is the provision of new physical parameters for improving the specificity in breast cancer diagnosis. This analysis describes a technique to extend the reconstruction to anisotropic elastic properties in terms of a so-called transversely isotropic model. Viscosity is treated as being isotropic. The particular model chosen for the anisotropy is appealing because it is capable of describing elastic shear anisotropy of parallel fibers. The dependence of the reconstruction on the particular choice of Poisson's ratio is eliminated by extracting the compressional displacement contribution using the Helmholtz-Hodge decomposition. Results are presented for simulations, a polyvinyl alcohol breast phantom, excised beef muscle, and measurements in two patients with breast lesions (invasive ductal carcinoma and fibroadenoma). The results show enhanced anisotropic and viscous properties inside the lesions and an indication for preferred fiber orientation.

Algorithms↗
PubMed2004

An experimental infection model for Tetrameres americana (Cram 1927).

An experimental infection model for the heteroecious spiruid nematode Tetrameres americana (Cram 1927) was developed. The cockroach Blattella germanica (L.) and the locust Locusta migratoria (L.) were found to serve as intermediate hosts for the parasite. T. americana larvae developed to full maturity in these intermediate hosts and were infective to young Lohman Brown chickens after 32 days in the cockroach and 28 days in the locust. The maximum length of the larvae was reached in the insects at 28-30 degrees C after 10-15 days, at which time the larvae measured up to 2.2 mm. The parasite did not develop in the cockroach Periplaneta americana (L.), the woodlouse Oniscus asellus (L.), or the pupal stage of the giant mealworm Zophobas morio (Fabricius). Trials in which chickens were infected directly without an intermediate host failed. Infection of 24 chickens with a dosage of 100 larvae was followed by weekly post-mortems until day 48 post-infection (p.i.) and used to describe the development of T. americana. The average establishment rate (%) and the average worm burden varied from 16.5 to 30.8. The total numbers of parasites recovered ranged from 9 to 40. During mating, in the first 2 weeks p.i. females and males were equally abundant, whereas from day 20 p.i. twice as many females were recovered. From day 13 p.i. the females average length fluctuated between 2.6 and 3.7 mm, whereas they reached their maximum width of 2.4 mm on day 48 p.i. Males reached their full length after 27 days p.i. and measured up to 6.7 mm.

Animals↗
PubMed2004

Tetrameres americana Cram (1927) populations in chickens infected with different dose levels.

Three groups of 20-45 Lohman brown chickens aged 3 weeks were orally infected with doses of 25, 100 and 400 Tetrameres americana, respectively. Fifteen chickens were kept as uninfected controls. Every third week in a period of 12 weeks, 5-15 chickens were slaughtered and the proventriculi were examined for the presence of adult stages of T. americana. From day 21 post-infection, pooled feacal samples were examined for parasite eggs, whereas the weight gain of the chickens was monitored weekly. The parasite established the infection in similar rates in all the three groups, 9.5-15.2%, except on day 84 post-infection, when the establishment rate of the high-dose group was significantly lower (P < 0.005). The average worm burden increased with increasing dosages, though displaying the worm burden of the high-dose group as being roughly halved on day 84 post-infection, thus suggesting an expulsion of worms. Females were more abundant than males. The mean lengths of male and female specimens showed no significant differences between the groups. The egg output was also increased with increasing dosages with the earliest prepatent period of 38 days post-infection found in the high-dose group. Infected chickens exhibited no difference in weight gain in comparison with the controls. This study demonstrated that single infections with varying doses influenced the establishment rate and the worm burden but not the parasites egg excretion, worm size nor the weight gain and mortality of the final host.

Animals↗
PubMed2004

Time reversal of electromagnetic waves.

We report the first experimental demonstration of time-reversal focusing with electromagnetic waves. An antenna transmits a 1-micros electromagnetic pulse at a central frequency of 2.45 GHz in a high-Q cavity. Another antenna records the strongly reverberated signal. The time-reversed wave is built and transmitted back by the same antenna acting now as a time-reversal mirror. The wave is found to converge to its initial source and is compressed in time. The quality of focusing is determined by the frequency bandwidth and the spectral correlations of the field within the cavity.

Journal Article↗
PubMed2004

Measuring of viscoelastic properties of homogeneous soft solid using transient elastography: an inverse problem approach.

Two main questions are at the center of this paper. The first one concerns the choice of a rheological model in the frequency range of transient elastography, sonoelasticity or NMR elastography for soft solids (20-1000 Hz). Transient elastography experiments based on plane shear waves that propagate in an Agar-gelatin phantom or in bovine muscles enable one to quantify their viscoelastic properties. The comparison of these experimental results to the prediction of the two simplest rheological models indicate clearly that Voigt's model is the better. The second question studied in the paper deals with the feasibility of quantitative viscosity mapping using inverse problem algorithm. In the ideal situation where plane shear waves propagate in a sample, a simple inverse problem based on the Helmholtz equation correctly retrieves both elasticity and viscosity. In a more realistic situation with nonplane shear waves, this simple approach fails. Nevertheless, it is shown that quantitative viscosity mapping is still possible if one uses an appropriate inverse problem that fully takes into account diffraction in solids.

Algorithms↗
PubMed2004

Monitoring thermally-induced lesions with supersonic shear imaging.

Thermally-induced lesions are generally stiffer than surrounding tissues. We propose here to use the supersonic shear imaging technique (SSI) for monitoring high-intensity focused ultrasound (HIFU) therapy. This new elasticity imaging technique is based on remotely creating shear sources using an acoustic radiation force at different locations in the medium. In these experiments, an HIFU probe is used to generate lesions in fresh tissue samples. A diagnostic transducer, controlled by our ultrafast scanner, is located in the therapeutic probe focal plane. It is used for both generating the shear waves and imaging the resulting propagation at frame rates reaching 5,000 images/s. Movies of the shear wave propagation can be computed off-line. The therapeutic and imaging sequences are interleaved and a set of wave propagation movies is performed during the heating process. From each movie, elasticity estimations have been performed using an inversion algorithm. It demonstrates the feasibility of detecting and quantifying the hardness of HIFU-induced lesions using SSI.

Algorithms↗
PubMed2003

Observation of shock transverse waves in elastic media.

We report the first experimental observation of a shock transverse wave propagating in an elastic medium. This observation was possible because the propagation medium, a soft solid, allows one to reach a very high Mach number. In this extreme configuration, the shock formation is observed over a distance of less than a few wavelengths, thanks to a prototype of an ultrafast scanner (that acquires 5000 frames per second). A comparison of these new experimental data with theoretical predictions, based on a modified Burger's equation, shows good agreement.

Journal Article↗
PubMed2003

High power transcranial beam steering for ultrasonic brain therapy.

A sparse phased array is specially designed for non-invasive ultrasound transskull brain therapy. The array is made of 200 single elements corresponding to a new generation of high power transducers developed in collaboration with Imasonic (Besançon, France). Each element has a surface of 0.5 cm2 and works at 0.9 MHz central frequency with a maximum 20 W cm(-2) intensity on the transducer surface. In order to optimize the steering capabilities of the array, several transducer distributions on a spherical surface are simulated: hexagonal, annular and quasi-random distributions. Using a quasi-random distribution significantly reduces the grating lobes. Furthermore, the simulations show the capability of the quasi-random array to electronically move the focal spot in the vicinity of the geometrical focus (up to +/- 15 mm). Based on the simulation study, the array is constructed and tested. The skull aberrations are corrected by using a time reversal mirror with amplitude correction achieved thanks to an implantable hydrophone, and a sharp focus is obtained through a human skull. Several lesions are induced in fresh liver and brain samples through human skulls, demonstrating the accuracy and the steering capabilities of the system.

Brain↗
PubMed2003

In vivo breast tumor detection using transient elastography.

This paper presents first in vivo experiments for breast tumor detection using transient elastography. This technique has been developed for detection and quantitative mapping of hard lesions in soft tissues. It consists in following the propagation inside soft tissues of very low-frequency shear waves (approximately 60 Hz) generated by a vibrating system located at the body surface. Because transient shear waves propagate through the medium in less than 0.1 s, the shear propagation imaging is performed with an ultrafast echographic scanner able to reach frame rates up to 6000 Hz. The local shear wave speed is directly linked to the local shear Young's modulus of the medium. The shear elasticity map of the medium can then be computed using an inversion algorithm. In vivo experiments were conducted on 15 women who had palpable breast lesions. For clinical adaptability reasons, shear waves were generated by the surface of the ultrasound (US) imaging transducer itself, which was linked to a mechanical vibrator. Our preliminary in vivo results demonstrate the clinical applicability of the transient elastography technique for breast lesion detection.

Adenocarcinoma↗