Biomedical subjects
Publications and source records attributed to M Filip.
High-altitude retinopathy is a very rare ocular disease in our country, which can occur isolately or as a part of high-altitude illness. This paper presents the case of a patient with high-altitude illness and the diagnosis and treatment problems of this case.
Choroidal neovascularization (CNV) may occur in any ocular condition affecting the integrity of the choriocapillaris-Bruch's membrane-retinal pigment epithelium-outer retinal layer complex. CNV in the macula is a major cause of severe central visual loss. A variety of clinical symptoms suggest the diagnosis of CNV. Fluorescein and indocyanine green angiography play a very important role in the detection and precise localization of CNV in relation to the centre of the foveal avascular zone. In the present, CNV benefits by many treatment possibilities. The goal of treatment is specifically to decrease the risk of visual acuity loss.
Dopamine D(1)/D(5) receptor antagonism has been shown to block the euphoric and stimulatory effects of cocaine in humans and rats. In the present study, rats trained to discriminate the presence of cocaine (10 mg/kg) from its absence were used to analyze the functional contribution of D(1) (D(1)R) versus D(5) (D(5)R) receptors in the nucleus accumbens, an important neural site for the actions of cocaine. Bilateral microinfusion into the nucleus accumbens of an antisense oligonucleotide directed at the D(5)R (0. 75 nmol/0.3 microl per side, two times per day for 3 d) elicited a downward shift in the dose-effect curve for cocaine with a suppression of peak efficacy; the dose of cocaine estimated to elicit 50% drug-lever responding (ED(50)) was 6.71 mg/kg when assessed 12 hr after the D(5)R antisense oligonucleotide compared to the control ED(50) of 1.83 mg/kg and to the ED(50) of 1.75 mg/kg established 7 d after the last D(5)R antisense oligonucleotide infusion. The D(1)R antisense and scrambled oligonucleotide (0.75 nmol/0.3 microl per side, two times per day for 3 d) were both ineffective. Thus, using drug discrimination techniques that model the subjective effects of cocaine, we show that responsiveness to cocaine is dramatically attenuated after interference with the process of translation of the D(5)R mRNA to its protein product. These findings suggest that D(5)R is a functionally important target site for the indirect actions of cocaine and that rigorous investigations of the function of D(5)R may help guide the discovery of strategies for pharmacotherapy in cocaine dependence.
To examine the role of glycineB receptors in the stimulus effects induced by psychostimulants, separate groups of rats were trained to discriminate amphetamine (AMPH; 1 mg/kg) from saline (SAL), or cocaine (COC; 10 mg/kg) from SAL, using a two-lever operant procedure. Substitution studies showed that neither 1-aminocyclopropanecarboxylic acid (ACPC; 200 mg/kg) nor 7-chloro-4-hydroxy-3-(3-phenoxy)phenyl-(H)quinolone (L-701,324; 3 mg/kg), being a partial agonist or an antagonist at glycineB receptors, respectively, generalized for the training drugs. Combination tests of glycineB ligands demonstrated that injection of a fixed dose of ACPC (200 mg/kg) or L-701,324 (3 mg/kg) together with different doses of AMPH or COC practically did not modify dose-response curves of the psychostimulants, nor did it affect their ED50 values. Our results indicate that glycineB receptors do not play a role in the discriminative effects of AMPH and COC.
The effects of serotonin (5-HT)1A drugs on the development and expression of sensitization to the locomotor effect of amphetamine (AMPH) were studied in mice. 8-Hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), a 5-HT1A agonist, dose-dependently reduced the expression of AMPH (2.5 mg/kg)-induced sensitization. The latter inhibitory effect of 8-OH-DPAT was reversed by (S)-N-tert-butyl-3-(4-(2-methoxyphenyl)piperazin-1-yl)-2-phenyl propamine (WAY 100135), a 5-HT1A antagonist. WAY 100135 given alone did not affect expression of AMPH sensitization. Combined injections of 8-OH-DPAT, but not WAY 100135, with AMPH (2.5 mg/kg) during the development of sensitization, protected against the expression of sensitization to a challenge dose of AMPH (2.5 mg/kg) 3 days after withdrawal. The above inhibitory effect of 8-OH-DPAT on the development of AMPH sensitization was blocked by pretreatment with WAY 100135. The AMPH-induced conditioned locomotion was unaffected by pretreatment with 8-OH-DPAT. These results indicate that 5-HT1A receptors are not involved in AMPH-induced sensitization per-se, whereas their pharmacological activation leads to the inhibition of both the development and the expression of AMPH-induced sensitization.
Effects of adrenalectomy (ADX) and corticosterone (CORT) on the development and expression of sensitization to the locomotor effect of cocaine (COC) were studied in rats. Sensitization was evoked by 5 daily injections of COC (10 mg/kg) and measured after a challenge dose of the drug (10 mg/kg) after a 5-day withdrawal (on day 10 of the experiment). ADX, performed before the start of COC administration, completely blocked the manifestation of COC-induced sensitization. In contrast, ADX performed on animals already sensitized to COC did not affect the sensitized locomotor activity response to a challenge dose of COC (on day 18). Pretreatment with CORT, 10 mg/kg, but not 5 mg/kg, before each of the 5 daily COC injections facilitated the development of COC sensitization, tested after a 5-day withdrawal. When pretreated with CORT alone (10 mg/kg), the challenge dose of COC administered on day 10 induced cross-sensitization to CORT. CORT (10 mg/kg) injected acutely before COC on day 10, potentiated the expression of COC sensitization. When given alone, on day 10 CORT (5-10 mg/kg) induced an increase in the locomotor activity of rats pretreated daily (5 injections) with COC. No drug treatment induced conditioned locomotion, as measured after saline challenge on day 8. Our results indicate that CORT facilitates the development and expression of COC sensitization, while ADX blocks the initiation of the behavioral phenomenon only. Moreover, there takes place cross-sensitization between CORT and COC, which indicates a close relationship between the drug-related mechanism and behavioral sensitization.
BACKGROUND: A small but significant increase in myopia after extended wear of low oxygen permeability (Dk) hydrogel lenses has been previously reported; however, the specific impact of hypoxia on refractive status and corneal curvature with extended wear are not well documented. THE PURPOSE of this study was to compare the refractive changes induced over a period of 9 months' extended wear with high-Dk fluorosiloxane hydrogel lenses and low-Dk hydrogel lenses. METHODS: Adapted daily wear contact lens wearers were randomly assigned to one of two groups. The low-Dk group wore etafilcon A (Dk = 28) for up to 7 days and 6 nights and the high-Dk group wore lotrafilcon A (Dk = 140) for up to 30 days and nights. Refractive error and corneal curvature were measured at 3-month intervals over 9 month of extended wear. RESULTS: The etafilcon A group demonstrated an average increase in myopia of 0.30 D over the 9 months period; however, no change in spherical myopic correction was measured in the lotrafilcon A group. The cylindrical component did not change in either group. A stratified analysis revealed a greater increase in myopia for low myopes than moderate myopes in the etafilcon A group but no difference in the lotrafilcon A group. Keratometric analysis revealed no change in the etafilcon A group and a small degree of central corneal flattering in both major meridians of 0.35 D in the lotrafilcon A group. CONCLUSIONS: Nine months of extended wear of low-Dk lenses is associated with a small degree of myopic progression in adult myopes that appears to be reversible. Wearing fluorosiloxane-hydrogel lenses of high-Dk had no impact on refractive error and may be associated with a small degree of central corneal flattering.
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In the beginning, the paper presents some general words about the main intraoperative complications of the phacoemulsification and some practical attitudes patient in front of the rupture of the posterior capsule. The second part presents the case of a 63 years old patient who was intended to be operated for cataract by phacoemulsification and who suffered a complication during this procedure--the rupture of the posterior capsule with the luxation of the majority of the nucleus into the vitreous body.
The paper reports a retrospective study performed between January 1996 and January 1999 which comprised 423 patients who underwent eye cataract surgery. Vitreoretinal complications (retinal detachment, cystoid macular edema, choroidal detachment, endophthalmitis, luxation of the lens into the vitreous body) were related to age, sex, other general and local diseases and surgical complications, like disruption of the posterior capsule. The study presents also vitreoretinal complications after Nd:YAG laser posterior capsulotomy. The outcomes of the study show that 18 cases (4.25%) had retinal detachment, 11 (2.60%) had cystoid macular edema, 2 cases had endophthalmitis, while choroidal detachment and luxation of the lens into the vitreous body were reported each in 1 case. Retinal detachment following Neodymium:YAG laser capsulotomy (performed in 109 cases) was found in 2 cases. All of those vitreoretinal complications were reported with a higher incidence in patients with vascular diseases (diabetes mellitus, arterial hypertension, angiosclerosis) and also in patients who had axial lengths of 25 mm or greater.
To examine the role of the nitric oxide (NO) pathway in the stimulus effects induced by some psychostimulants, separate groups of rats were trained to discriminate between amphetamine (AMPH; 0.5 mg/kg) and saline, or cocaine (COC; 5 mg/kg) and saline using a standard two-lever operant procedure. Substitution studies showed that AMPH and COC generalized for the training drugs in a dose-dependent manner, their ED50, values being 0.1 mg/kg and 1.2 mg/kg, respectively. The dose-response function of both those psychostimulants did not change in the course of the experiment. Moreover, AMPH and COC induced cross-substitution effects towards each other. Successive combination tests demonstrated that injection of a fixed dose of the NO synthase (NOS) inhibitor 7-nitro indazole (7-NI; 25 mg/kg) plus different doses of AMPH or COC resulted in a leftward shift in the dose-response curves of those psychostimulants and a decrease in their ED50 values. On the other hand, pretreatment with the NO donor molsidomine (MOL), injected in a fixed dose of 100 mg/kg before AMPH and COC, shifted the dose-response curves of the psychostimulants to the right and increased their ED50 values. Our results indicate that NO plays an inhibitory role in the dopamine (DA)-evoked discrimination effects of AMPH and COC in rats.
The inflammatory and infectious circumstances involving the orbit are known as 'orbitopathies' Considering the progress of immunology and imagistical investigations, it has been proposed a new classification of these entities. This classification divides the orbithopathies in specific and nonspecific. This paper brings up the case of a 65 year old female patient, showing unilateral proptosis with progressive evolution for about one year. The local examination and paraclinical investigations (computerised tomography and thyroid gland scintigram) sustained the diagnosis of Basedow disease. The ophthalmopathy in Basedow disease is a specific orbital involvement. Considering it's high rate of occurrence among the exophthalmic eyes (10%) it rests a severe disease, with a not yet fully understood physiopathology and a controversial treatment. The basedowian ophthalmopathy is, as well, an autoimmune disease, which requires the presence of T lymphocytes at the level of the orbital tissue. The computerised tomography gives us a direct visualisation of the enlarged muscles in hyperthyroidism (the muscles can increase 8 times their normal size), that is why we have to recommend it every time we are facing a patient with proptosis, especially unilateral.
Technological advances in the design and manufacture of soft toric contact lenses have made it possible for most astigmatic patients to achieve successful results with current soft lenses, but a small percentage of those patients are wearing them. We present the observations on 21 subjects that requested soft toric lenses in the past year. This lenses (TP 60) offered all the comfort of soft lenses and a good vision correction for astigmatism up to 6 diopters.
Choosing the proper artificial lens can be a problem, especially for the young surgeons, considering the fact that the "market" is so rich in possibilities. The present paper analyses the lens according to their dimension, form and material, in a perspective based on personal experience.
The influence of serotonin (5-HT)1A receptor stimulation on the dopamine (DA)-mediated locomotor and discriminative behaviours was evaluated in rats. The increased locomotor activity induced by the indirect DA agonist amphetamine (0.5 mg/kg) or cocaine (5 mg/kg) was dose-dependently inhibited by 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT; 0.125-0.5 mg/kg), a 5-HT1A agonist. (S)-N-tert-butyl-3-[4-(2-methoxyphenyl)piperazin-1-yl]-2-phenylpro panamide (WAY 100135; 10 mg/kg), a selective 5-HT1A antagonist, did not change the hyperactivity induced by amphetamine or cocaine, but it reduced the inhibitory effect of 8-OH-DPAT on the hypermotility evoked by either psychostimulant. In drug discrimination experiments, 8-OH-DPAT (0.125-0.5 mg/kg) did not antagonize the stimulus effects of amphetamine (0.5 mg/kg) or cocaine (5 mg/kg). When given in combination with amphetamine (0.025-0.5 mg/kg) or cocaine (0.25-5 mg/kg), 8-OH-DPAT (0.5 mg/kg) did not modify the dose-response curves of those psychostimulants. The obtained results indicate that 8-OH-DPAT inhibits the amphetamine- or cocaine-induced increases in the locomotor activity in rats via stimulation of 5-HT1A receptors. On the other hand, the lack of the antidiscriminative effects of 8-OH-DPAT suggests that the two effects of amphetamine and cocaine are not modified in the same way by 5-HT1A receptors.