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Biomedical subjects

M Fiala

Publications and source records attributed to M Fiala.

At least 109 records · Page 6Linked to original sources
PubMed1973

Cytosine arabinoside therapy for herpes simplex encephalitis--clinical experience with six patients.

Two neonates and four adults with herpes simplex virus (HSV) encephalitis were treated with cytosine arabinoside (Ara-C). A low dose of 40 to 160 mg per m(2) per day was given for 4 to 6 days by continuous intravenous infusion and, except in two cases, by intrathecal administration. In one patient, idoxuridine (IUdR) at the dose of 1 g every 4 h was also administered after 4 days of Ara-C therapy. Both neonates and two of four adults survived. Their clinical improvement was closely related in time to the onset of therapy with Ara-C (cases 1, 2, 3) and with IUdR (case 4). In one adult who died on the 27th day of illness of a massive pulmonary embolus, postmortem examination of the brain did not disclose viral inclusions, and viral culture was negative. In the other patient who died, however, brain culture postmortem was still positive for HSV despite 4 days of Ara-C therapy. Ara-C, in addition to IUdR, may be effective in HSV encephalitis treatment, but double-blind, controlled studies appear to be necessary with these agents.

Adult↗
PubMed1972

Susceptibility of herpesviruses to cytosine arabinoside: standardization of susceptibility test procedure and relative resistance of herpes simplex type 2 strains.

Susceptibility testing of herpes simplex type 1 (HSV-1) and type 2 (HSV-2) viruses to cytosine arabinoside (Ara-C) has been standardized under conditions of optimal reproducibility, susceptibility, and simplicity. Standard conditions were defined as virus inoculum of 10(1.5) to 10(2.5) TCID(50), WI-38 strain of lung fibroblasts, 2-day incubation, and observation of virus cytopathic effect after staining by the May-Greenwald-Giemsa method. The variability of minimum inhibitory concentration (MIC) of cytopathic effect was fourfold within each test and sixfold in successive assays. MIC values of all seven tested HSV-1 strains were in the range of 0.25 to 0.75 mug per ml, whereas four of nine HSV-2 strains had MIC values of 0.9 mug per ml and greater. A similar method can be used for assay of virus inhibitory titers of body fluids.

Cells, Cultured↗
PubMed1970

The rhinoviruses of man.

Rhinoviruses, prominent agents of the common cold syndrome in man, are small ribonucleic acid (rna) viruses resembling enteroviruses in their physicochemical properties except for high density and lability to acid pH. Rhinoviruses are propagated in human and monkey cells. Highest titers of virus are obtained in HeLa cell cultures. Rhinoviruses produce characteristic cytopathic effect in diploid fibroblasts. A plaque assay in HeLa cells is useful for their titration. The rhinovirus group includes many serotypes. Although rhinoviruses cause predominantly upper respiratory tract symptoms, they may on occasion infect the lower respiratory tract. Volunteers with specific antibody, when challenged with homotypic rhinovirus, are protected against the common cold.

Common Cold↗