Effects of potassium cyanide on silver stainability of specific cell structures.
Explore the source record for details and available documents.
Biomedical subjects
Publications and source records attributed to M Ferraro.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Clone-specific silver staining patterns of the nucleolus organizers (NOs) were observed in clones deriving from a human diploid fibroblast culture. Each specific staining pattern corresponds to one of the nine patterns observed in the general population. Since silver stainability of the NOs is related to rRNA gene function, these data demonstrate the clonal inheritance of rRNA gene activity.
In this paper we propose a model of visual perception in which a positive feedback mechanism can reproduce the pattern stimulus on a neurons screen. The pattern stimulus reproduction is based on informations coming from the spatial derivatives of visual pattern. This information together with the response of the feature extractors provides to the reproduction of the visual pattern as neuron screen electric activity. We simulate several input patterns and prove that the model reproduces the percept.
Five specific personality traits (emotivity, acceptance of sexual role, parental aptness, anxiety and depression) have been analyzed for this study of the premenstrual syndrome (PMS), conducted on a group of 110 women in advanced (8th month) pregnancy. The comparison of the results from the personality tests and from the overall assessment of the PMS (82%) establishes definite correlations between the syndrome's intensity and the tendency toward a pathologic personality. A further correlation of each personality trait and of the PMS shows that the greater deviation from normalcy affects not only those women who suffer from a severe PMS, but also those who complain of no premenstrual symptoms at all. Such a finding (as shown in the results of a separate previous study by our group) allows to conclude that a psychological normalcy or balance finds its equivalent in an absence, but more often in a scarce presence of premenstrual complaints, while an absolute absence or a very marked intensity of these complaints should correspond to the more extreme degrees of personality disturbance.
A method permitting rapid analysis of biological samples from patients suspected of being intoxicated is reported. The method is based on: a) extraction of the active principles of biological samples with chloroform; b) drying and preparation of a disc of KBr; c) spectrophotometric analysis; d) integration of data from laboratory analyses with the clinical data from the patient. A number of clinical cases are also reported in which adoption of the method permitted rapid diagnosis of the possible aetiology of the toxic pictures observed.
A case of pseudocyst of an asymptomatic right suprarenal gland found by chance is reported. Some aetiopathogenetic, anatomopathological and clinico-radiological aspects of the condition are discussed and, after reviewing the possible complications that may arise out of a suprarenal cyst, the value of surgery is stressed.
Chromosome analysis was performed in a case of mixed gonadal dysgenesis (MGD) with histological demonstration of both testicular structures and Müllerian derivatives. Mosaicism 45,X0/46, X plus a centric fragment was observed. C-, Q- and R-banding techniques show that the fragment has a terminal centromere and that it is derived from the short arm of the Y chromosome from the father. H-Y antigen was also shown to be present in cultured cells. These data demonstrate that both male-determining and H-Y genes are located on the short arm of the Y.
Chromosome analysis by Q, R, and C banding was performed in a case diagnosed clinically as gonadal dysgenesis and the karyotype was shown to be 46,X,Xt(qter leads to p221::p223 leads to qter). Localisation of the breakpoints in the fused X chromosomes and replication studies have led to a hypothesis on the origin of the translocation. A comparison of clinical and cytogenetical findings in this and other published cases has also been made in an attempt to detect some phenotype/karyotype correlations.
Simultaneous Q- and R-type banding patterns in human chromosomes have been achieved by staining with chromomycin A3. Some peculiarities of these patterns as compared to the patterns induced by other fluorochromes are described. The resolution power of this technique in analyzing structural rearrangements of human chromosomes is discussed.
Three cases of haemangioma of the parotid observed in infants are presented and their aetiopathogenetic and anatomopathological aspects are stressed. An examination of the pros and cons of conservative, surgical, radiation, and pharmacological management leads to the conclusion that surgery is the treatment of choice, provided a suitable technique is employed.
The frequency of involvement in satellite association and the frequency of selective staining of the secondary constrictions with silver solutions have been studied in five phenotypically normal individuals, all carriers of morphological variants of the nucleolus organizing region (NOR). The results show the preferential involvement of some morphological markers in satellite association, and also their preferential staining with Ag-I. It has also been shown that acrocentric chromosomes involved in satellite association are always stained by silver.
Explore the source record for details and available documents.
Three cases of morphologic variants of human D- or G-group chromosomes have been studied by N- and Ag-AS banding techniques. The results confirm our previous findings about the localization of nucleolus organizers on the secondary constrictions of acrocentric chromosomes. Preliminary results on the distribution and number of N bands in D- and G-group chromosomes with morphologic and fluorescence variants are reported.
Explore the source record for details and available documents.
A new case of translocation between chromosomes No. 5 and No. 12 is described. The translocation t(5;12) (5q15;12p13) is apparently balanced and was found in a child aged 3 1/2 years who was affected with severe psychomotor retardation. On the basis of the clinical picture it is suggested that the phenotype could be not dependent on the chromosomal alteration.
A No. 15 chromosome with a short arm longer than usual is observed in two phenotypically normal brothers. This chromosome appears to have no visible satellite, shows no N-band staining, and is never involved in satellite association. These results have led us to the conclusion that this chromosome lacks the nucleolus organizer region.
Explore the source record for details and available documents.