Biomedical subjects

M Ferrari

Publications and source records attributed to M Ferrari.

869 records · Page 49Linked to original sources

PubMedDate not supplied

[Comparison of detection methods for the Staphylococcus aureus capsule].

Eleven methods for capsule detection of Staphylococcus aureus were compared. The most suitable of them were transmission electron microscopy, determination of the presence of clumping factor, determination of colonial morphology in serum-soft agar, estimation of cell volume and staining with safranine. The determination of clumping factor is a fast and effective method for presumptive diagnosis of capsulated strains, but need to be confirmed by another method. The cell volume estimation is useful for determination of capsule production in liquid cultures, while staining with safranine is suitable for genetic studies of capsule production. The other methods analyzed in this work (Indian ink staining, use of anticapsular antisera, determination of virulence for mice, lisostaphin susceptibility, resistance to phages and resistance to phagocytosis) were laborious, too slow, or need components and/or equipment not available in all laboratories. In addition, two methods of induction of capsule production were assayed, one in vitro by several passages in broth with 10% bovine serum and the other in vitro by intraperitoneal inoculation in mice. Both methods induced capsule production.

Agglutination Tests↗

PubMedDate not supplied

Sealing capacity of a resin-modified glass-ionomer and resin composite placed in vivo in Class 5 restorations.

A 2% methylene blue dye was used to assess the leakage resistance of class 5 resin-modified glass-ionomer and resin composite restorations placed in 17 adults. The teeth were extracted 75 to 90 days after placement of the restorations and immersed for 24 hours in the dye solution. Within 7 days after extraction the teeth were sectioned inciso-apically through the center of the restoration and inspected under a stereomicroscope at X20 to determine the depth of dye penetration. No significant differences between the resin-modified glass-ionomer and resin composite restorations at the incisal or cervical margins were found. Although no more than 30% of the restorations of either group exhibited microleakage, neither of the restorative systems was able to completely prevent leakage at either incisal or cervical margins.

Adult↗

PubMedDate not supplied

All-ceramic fixed restorations: a preliminary clinical evaluation.

This article is a preliminary evaluation of a limited size clinical trial of all-ceramic fixed restorations. The maxillary anterior region in each of eight patients was restored with single-unit and three-unit fixed restorations, using a particular all-ceramic system. Patient selection, laboratory procedures, clinical techniques, patient follow-up, and the interim clinical results of the restorations, are presented and evaluated. The learning objective of this article is to review and update the knowledge of all-ceramic restorative materials and clinical procedures. Two of the eight cases in the study are presented to illustrate the clinical results.

Aluminum Oxide↗

PubMedDate not supplied

Comparison of in vivo and in vitro demineralized dentin with phosphoric and maleic acid.

This article evaluated possible differences between dentin conditioned in vivo and in vitro with 10 percent maleic acid or with 36 percent phosphoric acid. Semispherical Class V cavities were prepared in vivo and in vitro at the cementum-enamel junction and were divided into four groups. After etching procedures, the in vivo samples were extracted and fixated in 10 percent buffered formaldehyde. Both the in vivo and in vitro samples were then fractured in two parts along their long axis, critical-point dried, and subsequently examined with SEM. Both the acids removed completely the smear layer and demineralized the dentin, leaving a layer of collagenous network. No morphological differences were found between in vivo and in vitro dentin samples.

Acid Etching, Dental↗

PubMedDate not supplied

Measurement of catecholamines in mouse bone marrow by means of HPLC with electrochemical detection.

BACKGROUND AND OBJECTIVE: Noradrenergic innervation is present in the bone marrow and adrenergic agents can modulate hematopoiesis. However, since no data are available concerning endogenous catecholamines at this level, we investigated their presence and origin. METHODS: Using a high performance liquid chromatographic method, we have and measured endogenous catecholamines in bone marrow from normal, 6-OHDA-treated and pargyline-treated mice. RESULTS: Noradrenaline, adrenaline and dopamine levels were, respectively, 2806.74 +/- 408.85, 803.37 +/- 87.66 and 274.47 +/- 51.54 pg/g of tissue. Noradrenaline levels were lower after 6-OHDA (1130.47 +/- 142.73 pg/g of tissue, p < 0.01 vs. control values) and higher after pargyline (4122.62 +/- 509.54 pg/g of tissue, p < 0.05). None of these treatments significantly affected adrenaline or dopamine content. INTERPRETATION AND CONCLUSIONS: Noradrenaline in the bone marrow originates mainly from sympathetic nerve endings and is metabolized through specific enzymatic pathways. Adrenaline and dopamine may originate from other sources, such as the systemic circulation.

Adrenergic Agents↗