Biomedical subjects

M Fellous

Publications and source records attributed to M Fellous.

219 records · Page 13Linked to original sources

PubMedDate not supplied

[Production of monoclonal antibodies against HBs (author's transl)].

Two BALB/c mice were immunized 4 times with a mixture of adw2 and ayw4 subtypes of HBs antigens. Their spleens were then hybridized with mouse myeloma cell line NS1. Using three different radioimmunoassays (RIA), 264 independent hybridomas were screened for anti-HBs activity. By at last one of these techniques, 95% of the colonies were positive. Selected colonies were cloned and supernatants studied by RIA and immunodiffusion techniques for specificity characterisation. Some clones recognised the common "a" subtype, and other were directed to more restricted specificities. Ascites fluid was active on RIA up to 10(7) dilution (up to 29.000 UI/ml). These monoclonal antibodies may be powerful reagent for the diagnosis and understanding of viral hepatitis B.

Animals↗

PubMedDate not supplied

[Clinical, cytogenetical, histological, immunological and hormonal studies in a case of true hermaphroditism (author's transl)].

A true hermaphrodite with ambiguous genitalia and 46 XX caryotype was investigated during adolescence. At 13 years testosterone concentrations (ng/ml) were 6 and 390 respectively in peripheral and right ovotestis venous blood. After removal of the right gonad, large fluctuations of estradiol levels (40 to 220 pg/ml) were observed. But the testosterone secretion by the left ovotestis was low, although responsive to hCG. A significant LH surge was induced by ethinyl-estradiol load before removal of the left gonad. The 5 alpha-reductase activity was normal in the pubic skin and the levels of cytosolic receptors of testosterone and DHT were high in the left gonad. The presence of H-Y antigen was demonstrated on lymphocytes.

3-Oxo-5-alpha-Steroid 4-Dehydrogenase↗

PubMedDate not supplied

[Y chromosome and spermatogenesis].

World wide about 2% of men have a primary deficiency in sperm production. Familial cases of male infertility and chromosomal anomalies associated with this phenotype indicate that there is, at least in some cases, a genetic cause. In particular, deletions of the non-recombining portion of the Y chromosome are associated with a failure of sperm production. Deletion screening, using Y-specific markers has defined three regions (AZFa, b, c) that are associated with azoospermia. Each region contains one or more candidate genes that may be responsible for the phenotype. However, mutations have not yet been identified in any of these genes. This review discusses the structural organisation of the human Y chromosome and genes that are candidates for male infertility.

Genetic Testing↗