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Biomedical subjects

M Fazio

Publications and source records attributed to M Fazio.

At least 91 records · Page 5Linked to original sources
PubMed1980

Cell cycle-specific mutagenesis at the hypoxanthine phosphoribosyltransferase locus in adult rat liver epithelial cells.

The cell cycle specificity of chemical mutagenesis was studied by use of two cell synchronization techniques, one a nontoxic technique involving serum deprivation and the other a double thymidine block, to obtain rat liver epithelial cells in different phases of the cell cycle to be exposed to chemical mutagens. For both methyl methanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine, there was a cell cycle specificity of chemical mutagenesis, with the most sensitive phase being the period of DNA synthesis.

Cell Cycle↗
PubMed1978

Effect of benzylpenicillin on the synthesis and structure of the cell envelope of Neisseria gonorrhoeae.

The effect of benzylpenicillin on the synthesis and morphology of the cell envelope of Neisseria gonorrhoeae was examined. Penicillin immediately stopped murein synthesis; it also enhanced the rate of turnover of glucosamine, but not diaminopimelic acid, in the murein. In addition, penicillin greatly increased the shedding of lipid and lipopolysaccharide into the medium. In the electron microscope, protrusions of the cell membrane were evident, as well as apparent holes in the murein cell wall. All of these changes occurred while active synthesis was taking place, before the lysis of the cells. Lysis could be prevented by growing the cells at low pH and high concentrations of Mg2+; however, the effects of penicillin on murein synthesis and turnover and on the release of lipid were not affected.

Cell Membrane↗
PubMed1975

[Steroidal heterocyclic compounds: [16,15-c]-pyrazole derivatives of estradiol and of estrone].

Condensation between ethyl formate and estradiol 16-keto derivatives, in which the 17beta-hydroxyl groups had been protected as tetrahydropyranyl ethers, produced the corresponding 15-hydroxymethylene compounds. These, by cyclization with hydrazine, gave the [16,15-c]-pyrazole derivatives. Further reactions, such as hydrolysis, etherification and chromic oxidation, afforded many differently substituted [16,15-c]-pyrazoles of the estradiol and estrone series.

Chemistry, Pharmaceutical↗