Biomedical subjects
M Faulkner
Publications and source records attributed to M Faulkner.
Polypeptide patterns obtained by polyacrylamide gel electrophoresis of thylakoid membranes isolated from light and dark grown strains of Chlorogloea fritschii.
Sodium dodecyl sulphate-polyacrylamide gel electrophoresis has been used to determine the difference between thylakoid polypeptide patterns of light and dark grown strains of the cyanobacterium Chlorogloea fritschii. There were only 2 prominent bands present in the dark grown strains, polypeptide Mr50,000 and polypeptide Mr90,000, also five fainter bands in the Mr range 45,000-66,200 corresponding to photosystem one, compared with the 32 bands present in the light grown strains. There was no obvious indication of the Mr 33,000 3-(3,4-chlorophenyl)-1,1-dimethyl-urea binding protein. In addition the progressive daily development of the various photosystem components in the light and their relationship in photosynthesis was determined. It was observed that the increase of the relative concentrations of the photosystem two and phycocyanin components indicated their developments are mutually synchronized. The effect of light to dark and dark to light transfer on established strains was investigated. Appreciable loss of photosystem two components and the presence of an additional band Mr22,500 of unknown function in the light to dark transfer, and little reactivation of the photosynthetic capabilities in the dark to light transfer was observed.
Pneumocystis carinii pneumonia following 5-fluorouracil administration.
A 54-year-old man who had been treated with monthly courses of 5-fluorouracil for one year developed Pneumocystis carinii pneumonia. No evidence of significant, permanent, immunologic impairment was evident one year after the patient became infected. An infection associated with 5-fluorouracil treatment is implicated.
Nursing care study: a fighter from failure.
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Discrepancy between haemocytometer and electronic leucocyte counts in neutrophilic dogs.
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Electronic counting of dog leucocytes. Discrepancies arising from calibration with Coulter standard 4C and with the haemocytometer.
The size distributions of leucocytes in canine blood and in standard 4C are markedly different. The use of 4C to calibrate Coulter counters may result in the selection of a threshold setting for canine leucocytes which is too high. Repeated hand counting may be used as a method of calibration, but regular discrepancies occur between hand and electronic counts which are attributable to the differing lytic actions of the diluents used, acetic acid having a more marked effect than commercial Zapoglobin. Canine leucocytes did not show significantly increased lysis when subjected to Zapoglobin at approximately four times the standard concentration, but did do so on exposure to the standard concentration for longer than five minutes. The degree of discrepancy between hand and electronic counts varied in individual dogs suggesting that there is an inconstant leucocyte subpopulation which behaves differently in response to different lytic agents.
A rapid, semi-automated counting procedure for enumeration of antibody-forming cells in gel and nucleated cells in suspension.
A commercially available bacterial colony counter has been modified to permit rapid, highly accurate, semi-automated enumeration of antibody-producing plaque forming cells in semi-solid support medium as well as enumeration of nucleated cells in suspension on a standard hemacytometer chamber. This apparatus should therefore serve as an enormous time-conserving accessory to most modern laboratories involved in immunological research.
Role of cyclic AMP system in humorally stimulated gastric acid secretion.
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HEDIS audits: meeting the challenge.
The Health Plan Employer Data and Information Set (HEDIS) is a tool to measure and report managed care performance. HEDIS has become the industry standard for assessing and comparing the quality of managed care organizations (MCOs), so it is imperative that MCOs collect and communicate HEDIS data correctly and efficiently. Several primary issues face MCOs in compiling HEDIS data. In particular, this article examines the HEDIS audit process and outlines the preparation required to undergo an audit, common errors uncovered in the audit process, and steps that can be taken to ensure compliance with HEDIS technical specifications.
Silence does not mean acceptance.
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Thoracic aortic dissection in a patient with autosomal dominant polycystic kidney disease.
Autosomal dominant polycystic kidney disease is one of the most common hereditary diseases, and frequently has well defined extrarenal manifestations. Very few cases of aortic aneurysms associated with this disorder are described in literature. We report a 42-year-old male with autosomal dominant polycystic kidney disease presenting with dissecting aneurysm of the thoracic aorta.
Empowerment in policy and practice.
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Using video recording in observational research.
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