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Biomedical subjects

M F Scully

Publications and source records attributed to M F Scully.

122 records · Page 7Linked to original sources
PubMed1976

Fibrin subunits in venous and arterial thromboembolism.

The subunit fibrin composition of thrombi of both venous and arterial origin was examined by sodium dodecyl sulphate gel electrophoresis. The thrombi were recovered by surgical intervention and all had the same fibrin subunit composition. The alpha chains were cross-linked as alpha-chain polymers alpha (p), the gamma chains as gamma-chain dimers (gamma-gamma) while the beta chains were not crosslinked; a further subunit of molecular weight 33 000 was shown to be present in all the fibrins examined and was a degradation fragment of the beta or gamma chains. This data suggests that the crosslinked alpha chains are rate limiting to the lysis of thrombi in vivo. The digestion of pulmonary emboli by plasmin yielded soluble degradation products which were identified as D dimer and E, the latter fragments being the major products obtained by the lysis of in-vitro made plasma clots. The similarity of the composition and lysis of thrombus fibrin to that formed in vitro augurs well for the justification of in-vitro research on mechanisms in thrombolysis.

Electrophoresis, Polyacrylamide Gel↗
PubMed1976

Factors involved in the detection of pulmonary emboli by labelling techniques.

Experiments were set up to assess quantitatively the problem of detecting, by scanning and gamma-camera, labelled pulmonary emboli in the presence of background radioactivity. In vitro, a relative concentration of radioactivity of 2:1 was required to detect a 1-1 cm diameter "hot spot". The results in vivo suggest that the level of radioactivity of a pulmonary embolus situated in the centre of the lung field should be at least six times that of the circulating blood.

Animals↗