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Biomedical subjects

M F Peterson

Publications and source records attributed to M F Peterson.

6 recordsLinked to original sources
PubMed1999

The use of beta, gamma-methyleneadenosine 5'-triphosphate to determine ATP competition in a scintillation proximity kinase assay.

A novel method for characterizing the kinetics of protein kinase inhibitors is described. This method uses glycogen synthase kinase beta as the model protein kinase and looks at the shift in IC50 of inhibitors using the nonhydrolyzable ATP analog, beta, gamma-methyleneadenosine 5'-triphosphate, also known as AMP-PCP. Due to its inability to be hydrolyzed, AMP-PCP is being used to characterize known glycogen synthase kinase inhibitors by determining the shift in IC50 at concentrations above its calculated Ki of 490 microM. The assay format for the detection of inhibition is a scintillation proximity assay which is robust and reproducible at very low levels of [gamma-33P]ATP. The use of AMP-PCP coupled with the use of the scintillation proximity assay allows this characterization of inhibition without increasing [gamma-33P]ATP and without significantly diluting the overall assay signal. We have used this method in kinetic analyses to demonstrate that we can detect a significant shift in IC50 with the known ATP competitive inhibitors, staurosporine, Ro 31-8220, and olomoucine. The IC50 for glycogen synthase peptide and lithium chloride, which has been reported to be uncompetitive, remains unchanged.

Adenosine Triphosphate↗
PubMed1994

Skene's gland adenocarcinoma with increased serum level of prostate-specific antigen.

Skene's (periurethral) gland carcinoma is a rare neoplasm accounting for less than 0.003% of all genital tract malignancies in females. Generally, adenocarcinomas of the female urethra are assumed to arise from the periurethral glands, the female homologue of the prostate. A case of Skene's gland adenocarcinoma without mucosal urethral involvement is presented. The histologic features of this tumor closely resembled those of prostatic adenocarcinoma. In contrast, clear cell and columnar/mucinous variants of female urethral adenocarcinomas have been described previously. Perhaps this signifies different biologic processes in the development of Skene's/periurethral and urethral adenocarcinomas in females. Additionally, we performed immunohistochemical staining that was reactive for prostate-specific antigen (PSA). Preoperatively, the serum level of PSA was increased and promptly decreased after surgical excision of the lesion. Therefore, preoperative and postoperative monitoring of serum PSA titers in patients with adenocarcinomas of the female urethra or periurethral glands (or both) should be considered.

Adenocarcinoma↗
PubMedDate not supplied

Co-workers and hospital staff's work attitudes: individual difference moderators.

The specific implications of the patient care motives of health care staff are considered for the relationship between experiences with co-workers and attitudinal criteria. Personal attitudes toward co-workers (member attraction) and perceptions of strain among co-workers (group fragmentation) are found to be significantly related to intrinsic reward satisfaction and propensity to leave. The implications of individual differences are addressed by considering the moderating effects that internal-external control orientation and need for achievement have on the relationship between work group variables and work attitudes. Modest evidence is found that member attraction is more highly related to work attitudes for externals than for internals. Work group experiences are also found to be more highly related to work attitudes for technicians, aides, and orderlies, who have a low need for achievement than for others in this staff group. High need for achievement is found to be associated with high intrinsic reward satisfaction and low propensity to leave for both staff groups. It is cautioned that these findings may not generalize to many non-health-care settings. It is suggested that hospital supervisors should be sensitive to differences among individuals' responses to group pressures and stresses, even when precise personality measures are not available to them.

Achievement↗