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Biomedical subjects

M F Graham

Publications and source records attributed to M F Graham.

50 records · Page 3Linked to original sources
PubMed1984

An in vitro model of fibroplasia: simultaneous quantification of fibroblast proliferation, migration, and collagen synthesis.

Previous studies of fibroblast proliferation, migration, and collagen synthesis have been limited in their ability to define the interrelationship among these events in response to various inflammatory mediators. We have now defined an in vitro tissue culture model for the synchronous quantification of these parameters of fibroplasia. Biopsies (2 mm) of chicken flexor tendons are embedded in a fibrin matrix and exposed to various factors for 5 days in tissue culture. The availability of the fibrin matrix surrounding the tendon biopsy satisfies the need for a solid support medium for fibroblast migration. Multiple measurements of tendon fibroblast proliferation, migration into the fibrin matrix, and relative collagen synthesis are then made on these preparations. Fetal calf serum stimulated tendon fibroblast proliferation and migration in a dose responsive fashion, whereas the selective expression of collagen synthesis was decreased. Platelet lysate stimulated fibroblast proliferation at low concentration, but migration only at high concentration and was without effect on relative collagen synthesis. This model now provides a means of more clearly defining the influence of various inflammatory factors on the events of fibroplasia.

Animals↗
PubMed1982

Abnormal endometrial echoes: sonographic spectrum of endometrial pathology.

This report reviews the cases of 12 patients in whom ultrasound examination revealed endometrial cavity echoes that were more prominent than expected. Dilatation and curettage or hysterectomy, or both, in these patients revealed a spectrum of endometrial pathology in 83 per cent of the patients, including carcinoma in 33 per cent and normal findings in 17 per cent.

Adenocarcinoma↗
PubMed1981

Intrinsic tendon cell proliferation in tissue culture.

This study examines the potential of tendon cells to participate in tendon repair. Explants were prepared from chicken sublimis tendons and studied in tissue culture. Under defined culture conditions, in the absence of tendon sheath, tendon cells were observed to migrate in a limited concentric fashion around the margin of a 2 mm window in the explant. When a plasma clot was present in the window, tendon cell migration and proliferation were accelerated and continued until the window was confluent with cells. Electron micrographs demonstrated that these tendon cells were fibroblasts associated with extracellular collagen fibrils. Specific biochemical analyses confirmed that these tendon fibroblasts were synthesizing collagen. This in vitro study demonstrates that tendons contain active fibroblasts capable of proliferation, migration, and collagen synthesis. These findings support the hypothesis that tendons can heal by intrinsic processes.

Animals↗
PubMed1981

Ultrasonographic screening of the common hepatic duct in symptomatic patients after cholecystectomy.

This study was undertaken to assess the usefulness of an ultrasonographically enlarged common hepatic duct as an indicator of biliary obstruction after cholecystectomy. The abdominal sonograms of 165 postcholecystectomy patients were reviewed retrospectively, and follow-up was obtained on 56 patients. In 23 of 36 patients who had common hepatic ducts larger than 4 mm, the cause of biliary obstruction was found at surgery. In the remaining 13 patients, no evidence of obstruction was found on subsequent radiographic studies, and none of these patients underwent surgery. Eight patients had common bile ducts that were considered to be dilated (> 1cm) on subsequent intravenous cholangiography. In 20 patients whose common hepatic ducts measured 4 mm or less, 17 had no evidence of dilatation on subsequent radiographic studies, but three had choledocholithiasis.

Cholecystectomy↗
PubMed1980

Endoscopic small bowel biopsy in children with a modified multipurpose biopsy tube.

Small bowel biopsy is a difficult procedure in infants and children, and the authors describe a new technique. The Quinton multipurpose suction biopsy instrument is incorporated into an Olympus GIF-P2 fiberoptic endoscope to allow positioning of the biopsy capsule under direct visual control. Fourteen children, 2 months to 4 years of age, have been biopsied, and tissue was successfully obtained in 20 of 21 procedures. Nine of 21 endoscopic examinations of duodenal mucosa were abnormal and gave additional diagnostic information.

Biopsy↗
PubMed1980

The size of the normal common hepatic duct following cholecystectomy: an ultrasonographic study.

Sixty-seven asymptomatic patients underwent repeat ultrasonography four to 16 months following cholecystectomy with or without common bile duct exploration. Fifty-six patients (84%) had common hepatic ducts with a normal internal diameter of 4 mm or less. Four patients had common hepatic ducts measuring 5 mm, and seven patients had common hepatic ducts measuring 6 to 10 mm. It is concluded that, although the common hepatic duct generally is not dilated following cholecystectomy, there is a significant minority (16%) of asymptomatic patients who have common hepatic ducts larger than 4 mm, and in these patients the duct may measure up to 10 mm.

Cholecystectomy↗
PubMed1978

The usefulness of transfer factor in asthma associated with frequent infections.

Fifteen patients underwent controlled trial with transfer factor for repeated infections and severe asthma. Marked decrease in respiratory infections and striking improvement in asthma resulted. The authors suggest that transfer factor may reconstitute immune function, thus representing a unique approach to severe asthma associated with frequent infections.

Adolescent↗
PubMedDate not supplied

Inhibition of bile flow in the isolated perfused rat liver by a synthetic parenteral amino acid mixture: associated net amino acid fluxes.

To identify a role for amino acids in cholestasis associated with total parenteral nutrition, we measured bile formation by the isolated perfused rat liver in the presence and absence of added amino acids. All livers were infused constantly with sodium [14C]taurocholate (0.28 mumoles per min) for 90 min. At 40 min, a primed-constant infusion of a synthetic L-amino acid mixture (121 + 19.3 mumoles of N per min) was administered for an additional 50 min. Mean bile flow rates during the amino acid infusion were reduced from 15.4 microliter per min per 10 gm liver weight to 10.4 microliter per min per 10 gm (p less than 0.005). There was no significant change during saline infusion of control livers, and there was no significant difference in perfusate osmolalities in the two groups. Although biliary recovery of infused taurocholate was slightly lower in the experimental perfusions than in controls (95.3% vs. 101.7%, p less than 0.05), there was no significant reduction in taurocholate excretion rate during the infusion in either group. Bile flow changes were related to ambient concentrations and net fluxes of individual amino acids in the perfusate. Of the 14 infused amino acids, glycine and arginine achieved levels greater than 3 times greater than reported physiological postprandial portal venous concentrations in the rat, and together constituted about 25% of the 90-min perfusate amino acids (8.3 mM). The highest net hepatic uptake was for glycine (125 mumoles per hr per 10 gm), which was almost 50% of its infusion rate.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acids↗