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M F Desrosiers

Publications and source records attributed to M F Desrosiers.

At least 19 recordsLinked to original sources

An absorbed dose map of bone tissue treated with a radiopharmaceutical in vivo.

A beagle humerus treated with Ho-chelate radiopharmaceutical in vivo was examined by electron paramagnetic resonance (EPR) dosimetry. The bone was sectioned and the absorbed dose to each bone fragment was determined by additive re-irradiation of the bone tissue with calibrated doses of gamma radiation. The measured doses ranged from 4.3 Gy to 62 Gy. The highest doses were recorded in the predominately trabecular bone tissue and the lowest doses in the predominately cortical bone tissue. The mean absorbed dose for the entire bone was 17 Gy. The data from 50 bone fragments were combined to create an absorbed dose map of the interior bone surface.

Absorption↗

Barium dithionate as an EPR dosemeter.

Electron paramagnetic resonance (EPR) dosimetry is growing in popularity and this success has encouraged the search for other dosimetric materials. Previous studies of gamma-irradiated barium dithionate (BaS(2)O(6) x 2H(2)O) have shown promise for its use as a radiation dosemeter. This work studies in greater detail several essential attributes of the system. Special attention has been directed to the study of EPR response dependences on microwave power, irradiation temperature, minimum detectable dose and post-irradiation stability.

Barium Compounds↗

Individual biodosimetry at the natural radiation background level.

The present note describes new advancements to a valued tool in retrospective biodosimetry that lower the threshold of detection of radiation dose to 29 mGy. The advanced method is based on the electron paramagnetic resonance measurements of stable radiation-induced radicals in tooth enamel. Earlier this method had been used only for reconstruction of high radiation doses obtained accidentally. New opportunity of reconstruction of doses lower than 100 mGy opens a new realm of possibilities for assessing the health effects of ionizing radiation at the natural radiation background level.

Background Radiation↗

Current issues on EPR dose reconstruction in tooth enamel.

This paper focuses on the opportunities to gain risk assessment information from populations exposed to ionizing radiation. Details are presented on the scale of the nuclear events in several areas within the former Soviet Union. An overview of the issues associated with dose reconstruction by electron paramagnetic resonance (EPR) is given. Principles and outline of the EPR tooth enamel dose assessment are described. Technical details, the latest achievements and the problems of each procedural step are analyzed. In addition, the present accuracy of the EPR dose reconstruction, its problems and cost are discussed.

Dental Enamel↗

Cellular responses to endogenous electrochemical gradients in morphological development.

Endogenous electric fields give vectorial direction to morphological development in Zea mays (sweet corn) in response to gravity. Endogenous electrical fields are important because of their ability to influence: 1) intercellular organization and development through their effects on the membrane potential, 2) direct effects such as electrophoresis of membrane components, and 3) both intracellular and extracellular transport of charged compounds. Their primary influence is in providing a vectorial dimension to the progression of one physiological state to another. Gravity perception and transduction in the mesocotyl of vascular plants is a complex interplay of electrical and chemical gradients which ultimately provide the driving force for the resulting growth curvature called gravitropism. Among the earliest events in gravitropism are changes in impedance, voltage, and conductance between the vascular stele and the growth tissues, the cortex, in the mesocotyl of corn shoots. In response to gravistimulation: 1) a potential develops which is vectorial and of sufficient magnitude to be a driving force for transport between the vascular stele and cortex, 2) the ionic conductance changes within seconds showing altered transport between the tissues, and 3) the impedance shows a transient biphasic response which indicates that the mobility of charges is altered following gravistimulation and is possibly the triggering event for the cascade of actions which leads to growth curvature.

Electric Conductivity↗

Investigation of applicability of alanine and radiochromic detectors to dosimetry of proton clinical beams.

Cancer therapy studies using proton accelerators are underway in several major medical centers in the U.S., Russia, Japan and elsewhere. To facilitate dosimetry intercomparisons between these laboratories, alanine-based detectors produced at the National Institute of Standards and Technology and commercially available radiochromic films were studied for their possible use as passive transfer dosimeters for clinical proton beams. Evaluation of characteristics of these instruments, including the LET dependence of their response of proton energy, was carried out at the Institute of Theoretical and Experimental Physics. Results of absolute dose measurements were regarded as a preliminary step of dose intercomparison between ITEP and NIST. Measurements made in a number of experiments showed average agreement between the ITEP and NIST dosimetry standards to 2.5%.

Alanine↗

EPR dosimetry of cortical bone and tooth enamel irradiated with X and gamma rays: study of energy dependence.

Previous investigators have reported that the radiation-induced EPR signal intensity in compact or cortical bone increases up to a factor of two with decreasing photon energy for a given absorbed dose. If the EPR signal intensity was dependent on energy, it could limit the application of EPR spectrometry and the additive reirradiation method to obtain dose estimates. We have recently shown that errors in the assumptions governing conversion of measured exposure to absorbed dose can lead to similar "apparent" energy-dependence results. We hypothesized that these previous results were due to errors in the estimated dose in bone, rather than the effects of energy dependence per se. To test this hypothesis we studied human adult cortical bone from male and female donors ranging in age from 23 to 95 years, and bovine tooth enamel, using 34 and 138 keV average energy X-ray beams and 137Cs (662 keV) and 60Co (1250 keV) gamma rays. In a femur from a 47-year-old male (subject 1), there was a difference of borderline significance at the alpha = 0.05 level in the mean radiation-induced hydroxyapatite signal intensities as a function of photon energy. No other statistically significant differences in EPR signal intensity as a function of photon energy were observed in this subject, or in the tibia from a 23-year-old male (subject 2) and the femur from a 75-year-old female (subject 3). However, there was a trend toward a decrease (12-15%) in signal intensity at the lowest energy compared with the highest energy in subjects 1 and 3. Further analysis of the data from subject 1 revealed that this trend, which is in the opposite direction of previous reports but is consistent with theory, is statistically significant. There were no effects of energy dependence in the tooth samples.

Adult↗

A radiation accident at an industrial accelerator facility.

On 11 December 1991, a radiation overexposure occurred at an industrial radiation facility in Maryland. The radiation source was a 3-MV potential drop accelerator designed to produce high electron beam currents for materials-processing applications. This accelerator is capable of producing a 25 milliampere swept electron beam that is scanned over a width of 112.5 cm and which emerges from the accelerator vacuum system through a titanium double window assembly. During maintenance on the lower window pressure plate, an operator placed his hands, head, and feet in the beam. This was done with the filament voltage of the electron source turned "off," but with the full accelerating potential on the high voltage terminal. The operator's body, especially his extremities and head, were exposed to electron dark current. In an attempt to reconstruct the accident, radiochromic film and alanine measurements were made with the accelerator operated at two beam currents. Measured dose rates ranged from approximately 40 cGy s-1 inside the victim's shoe to 1,300 cGy s-1 at the hand position. Approximately 3 mo after the accident, it was necessary to amputate the four digits of the victim's right hand and most of the four digits of his left hand. Electron paramagnetic resonance spectrometry, which measures the concentration of radiation-induced paramagnetic centers in calcified tissues, was used to estimate the dose to the victim's extremities. A mean dose estimate of 55.0 +/- 3.5 Gy (95% confidence level) averaged over the mass of the bone was obtained for the victim's left middle finger (middle phalanx).

Accidents, Occupational↗

Jamaican and American adult perspectives on child psychopathology: further exploration of the threshold model.

Although several factors determine whether children receive psychological intervention, cultural determinants may be particularly influential. Cultural factors may influence adults' levels of concern over child psychopathology. This possibility was explored by comparing adult attitudes in two socioculturally different societies. Jamaican and American parents, teachers, and clinicians (total N = 382) judged vignettes of two children, one with overcontrolled (e.g., fearfulness) and one with undercontrolled (e.g., fighting) problems. Regression analyses revealed that although years of education affected some adult ratings, culture had the most profound effect.

Attitude↗

Radiation doses.

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Animals↗

Estimation of the absorbed dose in radiation-processed food--1. Test of the EPR response function by a linear regression analysis.

Free radicals produced in chicken bone tissue by 137Cs gamma-rays were measured using electron paramagnetic resonance spectroscopy. The yield of radicals was found to be proportional to the absorbed dose. Additive re-irradiation of previously irradiated bones is the basis of a method to estimate the absorbed dose in radiation-processed foods. The ability of the method to provide accurate dose assessments for a range of doses (0.5-7.4 kGy) is tested here. A linear fit to the data yields reasonable dose estimates for bone irradiated less than 2 kGy, but fails at the higher doses using a linear approximation to the dose response. These data and their implications are discussed.

Animals↗

Estimation of the absorbed dose in radiation-processed food--2. Test of the EPR response function by an exponential fitting analysis.

The use of electron paramagnetic resonance spectroscopy to accurately evaluate the absorbed dose to radiation-processed bones (and thus meats) is examined. Additive re-irradiation of the bone produces a reproducible dose response function which can be used to evaluate the initial dose by back-extrapolation. It was found that an exponential fit (vs linear or polynomial) to the data provides improved accuracy of the estimated dose. These data as well as the protocol for the additive dose method are presented.

Animals↗

In vivo assessment of radiation exposure.

Three workers at a San Salvador 60Co irradiation facility were victims of a radiation accident, resulting in the amputation of a leg from two workers. The absorbed dose to bone was successfully assessed by electron paramagnetic resonance (EPR) quantitation of radiation-induced hydroxyapatite centers.

Accidents, Occupational↗

Mechanically-induced generation of radicals in tooth enamel.

Mechanical instrumentation of enamel leads to the formation of long-lived free radicals that can be conveniently measured by electron paramagnetic resonance (EPR) spectroscopy. Powdered enamel tissue exhibited EPR signals remarkably similar to the radicals formed by ionizing radiation. The observations described below lead to the conclusion that physical stress will induce a free-radical formation in dental tissues. These observations have significance for other areas of study such as dosimetry and archeological dating.

Dental Enamel↗

The effect of oxygen, antioxidants, and superoxide radical on tyrosine phenoxyl radical dimerization.

Dimerization of tyrosine phenoxyl radical yields bityrosine (BT) which can easily be monitored by its characteristic fluorescence at 400 nm. The reactivity of tyrosine phenoxyl radical with O2 was examined by a variety of techniques. BT fluorescence was measured as a function of O2 concentration. Over a range of pH values (4-12) there was no effect of oxygen on BT production ([O2] less than or equal to 0.72 mM). In addition, oxygen uptake by the phenoxyl radical was measured directly with an oxygen electrode. It was determined by this technique that oxygen does not react with the phenoxyl radical with a rate constant greater than 10(3) M-1 s-1. Tyrosine phenoxyl radical "repair" by superoxide and physiological antioxidants was examined by BT fluorescence quenching as well as pulse radiolysis. Implications of these results as to the fate of tyrosine phenoxyl radicals produced in biological systems is discussed.

Antioxidants↗