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Biomedical subjects

M F Cohen

Publications and source records attributed to M F Cohen.

17 recordsLinked to original sources
PubMed1992

Dynamic frequency change among stimulus components: effects of coherence on detectability.

Experiments were performed to determine the effect of coherent dynamic frequency change among stimulus components on detection of one of those components. Detectability of a frequency glide signal, centered at 660 Hz, was measured in the presence of two additional frequency glides centered at 220 and 440 Hz. Results show that the signal is most difficult to detect when it is changing coherently with the other stimulus components, and that detectability gradually improves as the frequency change among stimulus components becomes less coherent. A control experiment indicated that detectability of the signal is influenced somewhat by the average spectral distance between the signal and the other stimulus components. In order to separate the effects of dynamic frequency change from those of altering harmonic relationships, the experiment was repeated with stimulus components not harmonically related. Results are similar in pattern, though with somewhat smaller shifts. A final experiment was performed to determine if the observed shifts in detectability might be due to changes in the maximum spectral distance between signal and masker when their frequencies are changing incoherently. Results of this experiment indicate that detectability improves when frequency change of signal and masker is not coherent, even when maximum spectral distance does not change. These data indicate the likelihood that the auditory system is able to use coherent dynamic frequency change among stimulus components.

Adult↗
PubMed1991

Comodulation masking release and the masking-level difference.

An experiment was performed to determine if the mechanism that mediates comodulation masking release (CMR) is associated with that used to improve detection by the masking-level difference (MLD). The experiment consisted of first improving detectability of a masked diotic tone burst by adding a synchronous noise band at another frequency region (CMR), and then measuring an MLD in the usual manner, by inverting the tone-burst signal to one ear. Results indicate that a substantial MLD can be measured for a signal whose detectability has already been improved by CMR. However, that MLD (9 dB) is smaller than that measured in random noise (14 dB). Put another way, a small CMR (4 dB) can be produced even when the detectability of a stimulus has already been improved due to the MLD. These data are in general agreement with those of Hall et al. [J. Acoust. Soc. Am. 83, 1839-1845 (1988)] and Schooneveldt and Moore [J. Acoust. Soc. Am. 85, 262-272 (1989)].

Adolescent↗
PubMed1990

The cytoskeletal system of mammalian primitive erythrocytes: studies in developing marsupials.

Seeking to resolve conflicting literature on cytoskeletal structure in mammalian "primitive" generation erythrocytes, we have utilized the circulating blood of developing marsupials. In young of the Tammar Wallaby (Macropus eugenii) and the Gray Short-tailed Opossum (Monodelphis domestica), relatively large, nucleated primitive erythrocytes constituted nearly 100% of the circulating population at birth (= day 0) and in fetuses (Tammar) several days before birth. These cells were discoidal or elliptical, and flattened except for a nuclear bulge. Their cytoskeletal system, consisting of a marginal band of microtubules enclosed within a cell surface-associated network (membrane skeleton), closely resembled that of non-mammalian vertebrate erythrocytes. By day 2 or 3, much smaller anucleate erythrocytes of "definitive" morphology, lacking marginal bands, appeared in abundance. These accounted for greater than 90% of the circulating population of both species by day 6-8. Non-nucleated erythrocytes of a different type, constituting 1-6% of the cells in most blood samples up to day 7, were identified as anucleate primitives on the basis of size, shape, and presence of a marginal band. Thus, loss of erythrocyte nuclei in mammals appears to begin earlier than generally recognized, i.e., in the primitive generation. Counts of these anucleate primitives in young of various ages implicated nucleated primitives as their probable source. Pointed erythrocytes, occasionally found in younger neonates of both species, occurred in greatest number in fetuses (Tammar) prior to birth. This is in accord with previous work on non-mammalian vertebrates suggesting that such cells are morphogenetic intermediates. The results confirm the long-suspected similarity between mammalian primitive erythrocytes and the nucleated erythrocytes of all non-mammalian vertebrates.

Animals↗
PubMed1987

The effect of cross-spectrum correlation on the detectability of a noise band.

An experiment was performed to study the interaction of two narrow-band noises having correlated temporal envelopes. The detection threshold of a 100-Hz-wide noise-band signal was measured at different center frequencies in the presence of a continuous 100-Hz-wide noise band having a center frequency of 1000 Hz. The two noise bands had either correlated or independent temporal envelopes. Measured signal detection thresholds are lower when the two noise bands are independent, but the magnitude of this difference is not a simple function of the frequency separation between the two noise bands.

Adult↗
PubMed1987

Masking effects in binaural detection and interaural time discrimination.

This study was designed to investigate the effects of masker level and frequency on binaural detection and interaural time discrimination. Detection and interaural time discrimination of a 700-Hz sinusoidal signal were measured as a function of the center frequency and level of a narrow-band masking noise. The masker was a continuous, diotic, 80-Hz-wide noise that varied in center frequency from 250 to 1370 Hz. In the detection experiment, the signal was presented either diotically (NoSo) or interaurally phase reversed (NoS pi). In the interaural time discrimination experiment, the signal level needed to discriminate a 30-microseconds interaural delay was measured. As would be expected, the presence of the masker has a greater effect on NoSo detection than NoS pi detection, and for masker frequencies at or near the signal frequency. In contrast, interaural time discrimination can be improved by the presence of a low-level masker. Also, performance improves more rapidly as the signal/masker frequency separation increases for NoSo detection than for interaural time discrimination and NoS pi detection. For all three tasks, significant upward spread of masking occurs only at the highest masker level; at low masker levels, there is a tendency toward downward spread of masking.

Adult↗
PubMed1987

Influence of place synchrony on detection of a sinusoid.

A series of experiments was performed to study the ability of the ear to code the temporal envelope of a waveform as demonstrated by comodulation masking release (CMR). The stimulus for all experiments was composed of a tone-burst signal, a 100-Hz-wide masker band centered at the signal frequency, and a second 100-Hz-wide noise band of variable frequency, the cue band. The cue band had a temporal envelope which was either correlated with or independent of that of the masker. The signal was a 100-Hz tone burst for most experiments. For the monotic stimulus, the correlated cue band results in lowered signal detection thresholds over a range extending from around 2/3 oct below the signal frequency to 1/3 oct above that frequency. When measured dichotically, with the signal and masker band in one ear and the cue band in the opposite ear, that effective range is expanded but the detection threshold shifts are a bit smaller. The greatest CMR is observed when the stimulus is presented diotically. With regard to effects of level and frequency, our data show CMR increasing with increasing stimulus level for a cue band lower in frequency than the signal, but show little effect of level for a cue band higher in frequency. Similarly, CMR increases with increasing stimulus frequency when the cue band is lower in frequency, but shows little effect of frequency for a cue band higher in frequency.

Auditory Fatigue↗
PubMed1985

The comparative effects of signal sensation level and sound-pressure level on interaural time discrimination.

A series of experiments was performed to examine the extent to which precision of interaural time discrimination depends on the sound-pressure level (SPL) and/or sensation level (SL) of the signal. All experiments used a tone burst signal and a continuous white noise masker, which was either diotic or interaurally phase reversed. Results of the first experiment indicate that (1) at equal signal SLs, interaural time and intensity discrimination is more precise when measured with the added diotic noise, and (2) addition of the phase reversed noise, previously shown to cause less precise interaural time discrimination, has a similar effect on interaural intensity discrimination. In the second experiment, interaural time JNDs for a signal of constant SPL were measured as a function of noise level. Results show that a low-level diotic noise can benefit interaural time discrimination, particularly at 500 Hz. The third and fourth experiments were performed to measure interaural time discrimination as a function of increasing signal SPL but constant signal-to-noise ratio. The data show the JND decreasing with increasing signal SPL at nearly the same rate with or without the added noise, indicating that an increase in signal-to-noise ratio is not necessary for improved discrimination.

Auditory Perception↗
PubMed1984

The effects of growth factors and serum on DNA synthesis and differentiation in thyroid cells in culture.

The effects of three putative growth factors and serum on [Me-3H]thymidine and Na125I incorporation into thyroid cell cultures have been examined. We found that serum and EGF could stimulate radioactively labelled thymidine incorporation into confluent cultures. However, both factors completely inhibited iodine uptake and organification at low concentrations. Insulin also stimulated [Me-3H]thymidine incorporation but had no adverse effect on thyroid differentiated function. TSH examined under the same conditions was not a growth factor but was essential to maintain differentiated functions. We conclude that thyroid growth and differentiation are not mutually exclusive processes. However, EGF and serum inhibit thyroid differentiated function at very low concentrations. Elucidation of the physiological role of these factors and their mechanism of action may lead to a greater understanding of thyroid hormone biosynthesis.

Animals↗
PubMed1982

Detection threshold microstructure and its effect on temporal integration data.

Auditory detection thresholds were measured at several preselected frequencies using an adaptive 2IFC procedure. Results confirm the existence of shifts in detection threshold ranging from 2 to 14 dB with quite small changes in signal frequency. There does not appear to be a uniform pattern associated with the microstructure of the detection threshold curve. An additional experiment was performed to determine the effect of signal duration on detection threshold microstructure. Results indicate that the temporal integration function is considerably steeper for more sensitive frequencies (3.7 dB/doubling of duration), than for less sensitive frequencies (1.7 dB/doubling). This probably is not due to differences in processing as much as it is to the effect of the energy spread associated with decreasing signal duration.

Acoustic Stimulation↗
PubMedDate not supplied

Direct regulation of Na(+)-dependent myo-inositol transport by sugars in retinal pigment epithelium: role of phorbol ester and staurosporin.

An Na(+)-dependent active process for myo-inositol (MI) uptake, sharing a common carrier system with glucose and sensitive to phlorizin, was previously established in primary cultures of bovine retinal pigment epithelial (RPE) cells (26, 32). The present report further examines the nature of glucose-induced inhibition of MI transport in primary cultures of RPE cells. RPE cells were grown in supplemented Dulbecco's modification of Eagle's medium (DMEM) containing 5 mM D-glucose (basic growth media) or 40 mM D-glucose or its nonmetabolizable analogue, alpha-methyl-D-glucoside (alpha MG); 1-5 mM nonradioactive MI, pyruvate, or lactate; or 0.2-20 microM phorbol 12-myristate 13-acetate (TPA) or straurosporin (modified growth media), for up to 4 weeks. The capacity of RPE cells to accumulate 3H-MI (ratios of intracellular transported radioactive MI, [MI]i, to external free MI concentration, [MI]i/[MI]o) decreased by up to 41% or 34% when cells were grown for 10 days or longer with 40 mM D-glucose or 40 mM alpha MG, respectively, compared to cells grown in basic growth media. The rate of uptake of 3H-MI also was reduced to 63 +/- 15% or 48 +/- 8% of the control values when cells were fed 1 or 5 mM nonradioactive MI, respectively. In addition, cellular capacity to bind to [3H]phlorizin was reduced to 52 +/- 7%, 61 +/- 5%, or 38 +/- 6% of the controls when RPE cells were fed 40 mM D-glucose, 40 mM alpha MG, or 5 mM nonradioactive MI, respectively. Growth media containing either pyruvate or lactate, the glucose metabolites, did not suppress the ability of RPE cells to accumulate MI. An 18 +/- 8% reduction in [3H]thymidine incorporation into DNA occurred when cells were grown in 40 mM glucose for 12-14 days, compared to cells grown with 5 mM glucose. Chronic treatment (12-14 days) of the cells with phorbol ester, an activator of protein kinase C, caused up to twofold increase in MI uptake, [3H]phlorizin binding, cell number, and DNA synthesis. However, when the rates of MI uptake into cells grown in basic growth media or TPA-treated media were normalized to cell number, no significant difference in MI uptake was found between the treated and untreated cells. Addition of staurosporin, a protein kinase C inhibitor, together with TPA, in the growth media reversed the phorbol-induced increase of MI uptake.(ABSTRACT TRUNCATED AT 400 WORDS)

Alkaloids↗