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Biomedical subjects

M F Beeler

Publications and source records attributed to M F Beeler.

At least 37 records · Page 2Linked to original sources
PubMed1980

Clinical evaluation of an algorithm for interpreting biochemical profiles showing hyperbilirubinemia.

An interpretive algorithm for biochemical profiles showing an elevated serum bilirubin concentration has been evaluated, modified, and retested in a retrospective clinical study, and is now being used at Charity Hospital in New Orleans to aid in the generation of interpretive comments to accompany the laboratory report. Further attempts to assess the clinical usefulness of this policy seem merited.

Computers↗
PubMed1980

Reasonableness of use of the serum isoenzymes creatine kinase and lactate dehydrogenase for the diagnosis of myocardial infarction at a medical center.

At Charity Hospital of Louisiana at New Orleans, in 1978, there was considerable underuse of serum creatine kinase and lactate dehydrogenase isoenzymes for the diagnosis of myocardial infarction. Laboratory generation of creatine kinase isoenzyme tests for patients suspected of having had myocardial infarctions was only partially effective in correcting underuse. It unnecessarily generated some overuse. Appropriate modifications of this policy could improve performance. Additional, effective educational efforts are needed to improve reasonable use of laboratory tests. The study uncovered an unexpected problem in the laboratory--in a significant percentage of cases, results of indicated and ordered tests failed to appear in the charts.

Clinical Enzyme Tests↗
PubMed1978

An improved assay for hexokinase activity in human tissue homogenates.

An improved method has been developed for the assay of hexokinase (EC 2.7.1.1) levels in human tissue homogenates. The enzyme is quantitated by the spectrophotometric measurement, at 340 nm, of NADPH formed according to the reaction scheme: [formula: see text] In tissue homogenates a number of enzymes are present which can interfere with the assay by reacting with substrates or products of the assay reactions. In the described procedure hexokinase is assayed directly in homogenates under conditions in which the effect of possible contaminating enzymes (glucose dehydrogenase, EC 1.1.1.47; glucose 6-phosphatase, EC 3.1.3.9; glucose phosphate isomerase, EC 5.3.1.9; 6-phosphogluconate dehydrogenase EC 1.1.1.44; and NADP-reducing enzymes) are eliminated. Precision studies on the assay gave within-day reproducibility of 4.3% (CV) on a tissue having a mean activity of 1.68 U/g of tissue, and day-to-day variability of 15% (CV) for a tissue averaging 1.83 U/g of tissue.

Glucose 1-Dehydrogenase↗
PubMed1978

Comparison of the conventional lecture method and the self-instruction method for teaching clinical pathology.

Two separate courses in clinical pathology were presented to two groups of second-year medical students. The first (large) group was taught by the conventional lecture method; the other (small) group, by a self-instructional method for roughly half of the course, followed by the conventional lecture method for the second half of the course. The effectivenesses of the two methods were compared, based on performances on the final written examination. It was found that the self-instructional method was as effective as the conventional method in teaching clinical pathology, at least in teaching basic principles and interpretive clinical chemistry, as judged by the performances of the two groups. Surprisingly, the two groups differed significantly in performances on the portion of the course taught by similar methods.

Education, Medical, Undergraduate↗
PubMed1977

Interpretation of the diagnostic biochemical profile.

Interpretation of the diagnostic biochemical profile has been assisted in some laboratories by simply listing on the chart paper the most common conditions causing increased and decreased concentrations of the substances measured, or by computer print-outs of a list of the most likely conditions causing the observed patterm. These are not always available, and in their absence the pathologist or the clinician or both must attempt interpretation on their own. We have prepared four algorithms (decision trees) as an aid to interpretion of the 12-test profile provided at Charity Hospital (which differs from the more usual ones by the inclusion of both creatinine and urea nitrogen and omission of cholesterol). The algorithms are still in the process of revision and development and thus should be regarded as tentative.

Alkaline Phosphatase↗
PubMed1977

CAP interlaboratory survey of analytic balances.

An interlaboratory survey of analytic balances was performed using aluminum oxide spheres weighing about 525 mg each. Results in 82 of the 83 laboratories completing the survey suggest that for this weight level, the error would not exceed +/-0.3% in 19 of 20 weighings; or +/-0.4% in 997 of 1,000. Discussions concerning components contributing to the variance are included.

Analysis of Variance↗
PubMed1976

Automated assay of ceruloplasmin by kinetic analysis of o-dianisidine oxidation.

Automated procedures for the kinetic assay of serum ceruloplasmin activity using a bichromatic and a centrifugal analyzer are described. The method is based on the oxidase activity of ceruloplasmin at pH 5.0 with o-dianisidine as substrate. Enzyme activity is reported in I.U./l, based on the molar absorption coefficient of o-dianisidine consumed. The substrate is stable and is not subject to non-enzymatic oxidation. Comparison with a manual reference end-point assay using the same substrate indicates good correlation of the bichromatic and centrifugal methods. The analytical precision is comparable to the manual assay for both methods.

Autoanalysis↗
PubMed1975

CAP survey to assess the extent of stray light problems in precision spectrophotometry.

When the absorbance at 240 nm. of a solution of sodium iodide was measured by 159 laboratories using narrow-band-pass spectrophotometers, 15% reported results that suggested a problem caused by stray light. The laboratories were also requested to measure the absorbance at 240 nm. of solutions of acid potassium dichromate and alkaline potassium chromate. The laboratories that had stray-light problems reported absorbance results that were skewed in distribution and showed low mean values and wide instrument-to-instrument variation. The laboratories without stray light problems reported results with less instrument-to-instrument variation, the results followed a symmetrical distribution, and the mean of the results provided an accurate estimate of the absorbance of the solutions.

Clinical Laboratory Techniques↗