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Biomedical subjects

M Emura

Publications and source records attributed to M Emura.

101 records · Page 6Linked to original sources
PubMed1976

Attempts to induce tubular deformations and subsequent mixed tumours from organotypic cultures of mouse renal mesenchyma.

With the aim of expanding knowledge on the pathogenesis of nephroblastomata, an embryological organ culture system (Grobstein, 1956) was tested for its applicability to in vitro carcinogenesis experiments by using murine sarcoma virus (MSV-M) and 3-methylcholanthrene (MCA). Treatment of CBA/H-T6 mouse metanephrogenic mesenchyma with MSV-M at the pretubular stage neither disturbed the glomerulogenesis nor induced rapid malignant transformation. Treatment of the same tissues with MCA considerably inhibited the glomerulogenesis but failed to also reduce rapid malignant transformation. However, one MSV-M, one MCA and two untreated cultures showed malignant transformation after prolonged survival in vitro and produced different histological types of tumours upon transplantation into newborn CBA/H-T6 mice.

Animals↗
PubMed1976

Malignant transformation and viral replication of rat bone and muscle cells after in vitro infection with rat-adapted murine sarcoma virus (Moloney).

Isolated bone and muscle cells from rat fetuses were infected with the rat-adapted osteosarcomogenic murine sarcoma virus (Moloney) for examination of malignant transformation and viral replication. After the infection, the bone cells underwent an unusual transformation characterized by two patients in the focus formation. In the early transformation (Transformation I), foci consisting of only morphologically altered cells appeared, but they soon disappeared. Focus formation of this type continued for subsequent cell passages. In the late transformation (Transformation II), typical foci of malignant cells were formed with rapid multiplication. The cells in both types of transformation produced sarcomogenic as well as leukemogenic viruses. They contained C-type particles showing varying morphology and dimensions. Distinct bone cell tropism of osteosarcomogenic murine sarcoma virus (Moloney) was shown for three in vitro passages through rat bone cells. The infected muscles cells produced Transformation I foci at an incidence lower than did the bone cells, being mostly nontransformed. The virus yielded from these cells was predominantly leukemogenic, consisting of typical C-type particles. Evidence presented suggests that the tissue dependency with replicating ability of murine sarcoma virus and murine leukemia virus present in osteosarcomogenic murine sarcoma virus (Moloney) preparation is responsible for exhibiting the tissue tropism of this virus.

Animals↗