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Biomedical subjects

M Eguchi

Publications and source records attributed to M Eguchi.

At least 127 records · Page 7Linked to original sources
PubMed1994

Amino acid sequence of an inhibitor from the silkworm (Bombyx mori) hemolymph against fungal protease.

A novel protein protease inhibitor (FPI-F) which is highly specific for fungal proteases and subtilisin was isolated from the silkworm hemolymph, and its amino acid sequence was determined by conventional methods. The inhibitor consisted of 55 amino acid residues and had a molecular weight of 6,100. The inhibitor included eight cysteine residues and relatively large amounts of acidic amino acids, but neither alanine, methionine nor tryptophan. The amino acid sequence of FPI-F was not homologous with those of other known protease inhibitors of microbe, plant or animal origin.

Amino Acid Sequence↗
PubMed1994

Bone marrow transplantation for erythroleukemia: a case report.

A 2 year old girl was diagnosed as having erythroleukemia (EL; M6 according to the French-American-British classification). After one course of low-dose cytosine arabinoside (Ara-C), complete remission was obtained. After three courses of low-dose Ara-C for consolidation, allogeneic bone marrow transplantation was performed from HLA-identical sibling. The course of post-transplantation was uneventful. Two years after transplantation, she continues to have durable engraftment and remission. In children with EL, conventional chemotherapy appears to be inadequate for producing durable long-term disease-free survival. Bone marrow transplantation should be considered in children with EL, in cases where suitable donors are available.

Bone Marrow Transplantation↗
PubMed1993

Ultrastructural localization of DNA in leukemic cells using osmium ammine B.

In order to determine whether there were any differences in distribution of nuclear DNA between acute lymphocytic leukemia (ALL) and acute myelogenous leukemia (AML), the localization of DNA in blasts from the bone marrow or buffy coat of 30 patients with ALL and 30 patients with AML was examined ultrastructurally by staining with osmium ammine B. By the ultrastructural cytochemistry, DNA in ALL cells was clumped in the nuclei, while in AML cells, it was dispersed. DNA had accumulated around the nucleoli of some blasts, and flecks of DNA were observed in nucleoli of a majority of blasts. The perinucleolar and intranucleolar DNA distribution could be classified into four types. The types with abundant perinucleolar DNA were frequently observed in ALL blasts, while the majority of AML blasts showed scant perinucleolar DNA. The types with intranucleolar flecks of DNA were more prominent in leukemic cells than in normal immature leukocytes. In conclusion, the pattern of distribution of DNA in the nuclei of leukemic cells differs between ALL and AML.

Adolescent↗
PubMed1993

Ultrastructural investigation of DNA in megakaryoblastic leukemia by using osmium-ammine-B: comparison with several types of leukemia.

Using DNA staining with highly stable osmium-ammine-B, the blasts of AMKL were observed under an electron microscope, in comparison with AML, ALL, CML-MK crisis and TMD. The DNA within the nucleus of the megakaryoblasts was observed as a high electron-density substance and tended to be uniformly dispersed within the nucleus. DNA, associated with nucleoli, could be roughly divided in four types based on the presence or absence of peri-nucleolar clumps and intra-nucleolar clumps. In cases of AMKL, we often observed a type that did not have peri-nucleolar DNA clumps but did have DNA flecks within the nucleoli. By analytical evaluation there were no differences among cells from several subtypes of megakaryocytic leukemia, such as AMKL in children with Down's syndrome, AMKL in children without Down's syndrome, AMKL in adults, and CML-MK crisis. The DNA distribution of TMD blasts, which were self-limited and not malignant, resembled that of AMKL blasts.

Adolescent↗
PubMed1993

Ultrastructural and ultracytochemical alteration of rat neutrophils induced by G-CSF.

Recombinant human granulocyte colony-stimulating factor (G-CSF) was administered intravenously to rats, and its effects on the neutrophils from bone marrow and peripheral blood were examined by electron microscopy. Immature cells such as the promyelocytes in the bone marrow of the rats 12 hours after G-CSF administration revealed more irregular nuclei than those in untreated rats. Forty-eight hours after G-CSF administration, these changes became more marked. In the peripheral blood, the number of cytoplasmic granules was increased 12 hours after administration of G-CSF. The nuclei of mature neutrophils at 48 hours showed hypersegmentation with slight chromatin aggregation. The peroxidase reaction observed by electron microscopy revealed an increase in the number of positive granules in the immature neutrophils 48 hours after G-CSF administration, and some of the granules tended to be large. Different from untreated granulocytes, a positive peroxidase reaction was observed in the perinuclear space and rough endoplasmic reticulum of mature cells in the peripheral blood 48 hours after G-CSF administration. These granules also tended to be large. The present electron microscopic investigations demonstrated alterations of the neutrophils in G-CSF-administered rats, and these cells retained ultracytochemical evidence of prematurity even at their mature stage.

Animals↗
PubMed1993

Protein-restricted diet prior to renal insult improves the recovery of renal function following ischemia.

The effects of a protein-restricted diet on renal recovery following renal ischemia were studied. The renal function was assessed by measuring the inulin clearance (CIN), the p-aminohippurate clearance (CPAH), and the percent fractional sodium excretion (%FENa) 24 h after 45 min renal ischemia. In rats fed with a regular diet (containing 19.6% protein), CIN was 10.0 +/- 2.2 microL/min/100 g body weight (BW), CPAH 0.08 +/- 0.02 mL/min/100 g BW, and %FENa 14.8 +/- 2.0, 24 h after renal ischemia. In contrast, feeding rats with a no-protein diet (0% protein) for 1 week prior to the ischemic insult significantly improved renal recovery (CIN 48.0 +/- 9.3 microL/min/100 g BW, CPAH 0.16 +/- 0.04 mL/min/100 g BW, and %FENa 2.43 +/- 0.58). Feeding rats with a no-protein diet for 3 weeks prior to ischemic insult further improved the renal recovery (CIN 113 +/- 30 microL/min/100 g BW, CPAH 0.47 +/- 0.17 mL/min/100 g BW, and %FENa 1.55 +/- 0.29). When rats fed with a regular diet were exposed to 45 min of ischemia, the survival rate on day 7 was 16.7%. In rats fed with the no-protein diet for 1 week and for 3 weeks, the 7-day survival rate was 100% in each case. The survival rate of rats fed for 3 days instead of 7 days with the no-protein diet was 87.5%. When a no-protein feeding was shortened to 1 day, no beneficial effects were observed and survival rate was 14.3%. (ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1993

Beneficial effects of a non-protein diet on renal function of rats exposed to ischemic and nephrotoxic insults.

We investigated the effects of a non-protein diet on renal recovery in rats following 45-minute renal ischemia and mercuric chloride (3 mg/kg BW:S.C.) poisoning. The rats were fed a non-protein diet for one week before the renal insults. Renal functions were measured 24 hours after renal ischemia or 6 hours after mercuric chloride administration. In the ischemia investigation, the glomerular filtration rate (GFR), the renal plasma flow rate (RPFR) and the percent fractional sodium excretion (%FENa) of rats fed a regular diet (19.6% protein) were 25 +/- 7 microliters/min/g KW, 0.19 +/- 0.1 ml/min/g KW and 14.8 +/- 2.0, respectively. These values in the rats fed a non-protein diet showed better recovery, returning to a GFR of 114 +/- 32 microliters/min/g KW, an RPFR of 0.37 +/- 0.1 ml/min/g KW, and a %FENa of 2.43 +/- 0.6, respectively (p < 0.05). Furthermore, the seven-day survival rate was improved from 17% in the regular diet group to 100% in the non-protein diet group. In the mercuric chloride investigation, the renal functions in rats on a regular diet were shown by a GFR of 461 +/- 51 microliters/min/g KW, an RPFR of 1.91 +/- 0.2 ml/min/g KW, and a %FENa of 2.22 +/- 0.5. One-week feeding with a non-protein diet ameliorated the decrease in renal function, resulting in a GFR of 604 +/- 84 microliters/min/g KW, an RPFR of 2.15 +/- 0.5 ml/min/g KW, and a %FENa of 2.20 +/- 0.6. Consequently, there was a distinct beneficial effect on the survival of these rats.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1993

[Cystic lymphangioma of the scrotum. Apropos of a case].

The authors present the case of a cystic lymphangioma in the scrotum, a tumor which usually occurs in the neck, but is rarely observed in this region. Patient was a 22 year-old man, admitted for fever and a mass within the scrotum. The tumor was completely resected and no recurrence has been observed after a 6 month follow-up.

Adult↗
PubMed1993

Long-term cinemicrography of erythroblasts from a patient with congenital dyserythropoietic anemia type III: direct observation of dysplastic erythroblast formation.

Erythroblasts from a patient with congenital dyserythropoietic anemia type III (CDA III) were observed by time-lapse phase-contrast cinemicrography. The erythroblasts were compressed with a new mechanical device; this procedure enhanced the visualization of their structures. Soret band micrography was carried out simultaneously to assess cytoplasmic hemoglobinization. The proliferation and differentiation course of the majority of CDA III erythroblasts was similar to that of normal erythroblasts. The formation of dysplastic erythroblasts showed two patterns of progression: (1) Usually, the process began with the appearance of a well-demarcated clear area in the nucleus of immature erythroblasts. This clear area reappeared in the progeny of the immature erythroblasts, at an increased incidence and in increasing size and rigidity. It finally came to occupy most of the nucleus, compartmentalizing it. The maturing cells displayed vigorous movement and progression of hemoglobinization and the nuclei became lobular; they grew to the cell size at which most CDA III erythroblasts divide, but did not do so. (2) Less frequently seen were abnormalities in mitosis and cellular behavior (e.g., tri- or bi-polar mitosis and subsequent fusion of daughter cells) or rapid growth of one daughter cell with concomitant shrinkage of the other.

Anemia, Dyserythropoietic, Congenital↗
PubMed1993

[Common acute lymphoblastic leukemia showing hypo-gamma-globulinemia and cerebral infarction due to cerebral artery obstruction].

A patient who developed hypo-gamma-globulinemia and cerebral infarction during the treatment for acute lymphoblastic leukemia (ALL) is reported. In this patient fever and rash during radio-therapy for central nervous system (CNS) prophylaxis and nausea and vomiting were observed during maintenance therapy. On the laboratory findings high level of the protein level in the cerebrospinal fluid and the eosinophil count of the peripheral blood were found in every examination. We attempted to isolate various viruses in consideration of the possibility of infection, but no virus could be detected. The effects of methotrexate (MTX) and l-asparaginase were also suspected. Since nausea and vomiting disappeared after discontinuation of MTX administration, the drug may had some effect, and the possibility of cerebral damage by MTX can not be excluded. Complication of irradiation are reported to occur often more than 10 years after the treatment, but this patient had the onset only 2 years after the treatment. Therefore, irradiation was unlikely to be responsible for the symptoms. The relation between the hypo-gamma-globulinemia and cerebral infarction was also unknown.

Adolescent↗
PubMed1993

An infant with both autoimmune neutropenia and idiopathic thrombocytopenia with IgG2/IgA deficiency.

We report an infant with autoimmune neutropenia (AIN), idiopathic thrombocytopenia (ITP), and IgG2/IgA deficiency. The patient was referred to our hospital at 5 months of age because of epistaxis and generalized petechiae. Physical examination revealed moderate hepatosplenomegaly. A complete blood count revealed a platelet count of 2.0 x 10(3) cells/microliters, and a white cell count of 3,600 cells/microliters, with severe neutropenia (less than 1% bands and segmented cells). Neutrophils and platelets adhering to megakaryocytes were decreased in the bone marrow. Tests for serum neutrophil-binding IgG (NB-IgG) and platelet-associated IgG (PA-IgG) were positive. A diagnosis of both AIN and ITP was made and therapy with intact-type gamma-globulin and prednisolone was initiated. Improvement occurred, but was temporary. A lack of serum IgA and IgG2 was noted during the clinical course. The patient has not been susceptible to bacterial infections but has had a severe clinical course with rubella and chickenpox.

Autoimmune Diseases↗
PubMed1992

Ultrastructural and ultracytochemical differences between megakaryoblastic leukemia in children and adults. Analysis of 49 patients.

BACKGROUND: Acute megakaryoblastic leukemia (AMKL) has two peaks in distribution of incidence (in adults and children 1 to 2 years of age) and is frequently seen in children with Down syndrome. The current study was undertaken to disclose whether there were any differences between these groups. METHODS: Electron microscopic and ultrastructural cytochemical features of 49 children and adults with a AMKL or chronic myelogenous leukemia (CML) in megakaryoblastic crisis were compared. RESULTS: Blast cells from children with AMKL, including those with and without Down syndrome, had immature features lacking typical alpha granules and a demarcation membrane system (DMS). However, blast cells from patients with AMKL with Down syndrome had more theta, electron-lucent, and basophil-like granules, suggesting that the blast cells had more potential to differentiate into other cell lines than megakaryocytes. The AMKL blast cells of adult patients showed a higher percentage of platelet peroxidase (PPO) positivity than other subgroups, and they occasionally contained typical alpha granules and DMS. This indicated that the blast cells of adults with AMKL were more mature than those of children and CML in megakaryoblastic crisis. CONCLUSIONS: By electron microscopic analysis, leukemic megakaryoblasts differed between children with AMKL with and without Down syndrome, adults with AMKL, and patients with CML in megakaryoblastic crisis.

Acid Phosphatase↗
PubMed1992

11;13 translocation in acute nonlymphocytic leukemia.

In this paper, we report on a 9-year-old girl with acute nonlymphocytic leukemia (FAB-M5) with a rare chromosome abnormality, t(11;23)(q21;p11). Peripheral blood showed Hb 7.5 g/dl, WBC 3,600 cells/microliters (10% blasts), and platelet count 110 x 10(3) cells/microliters. The bone marrow aspirates showed normal cellularity with 36.7% blasts. On morphological characteristics, micromegakaryocytes were observed, and on chromosome examination the karyotype was shown to be 46,XX,t(11;13)(q21;p11) in all metaphases.

Bone Marrow↗
PubMed1992

Pharmacological studies on 3-formylamino-7-methylsulfonylamino-6-phenoxy-4H-1-benzopyran-4-one (T-614), a novel antiinflammatory agent. 3rd communication: the involvement of bradykinin in its analgesic actions.

In order to elucidate the analgesic mechanism of 3-formylamino-7-methylsulfonylamino-6-phenoxy-4H-1-benzopyran-4-on e (T-614), its effects on the kinin-forming system were examined both in vivo and in vitro. T-614, at doses more than 10 mg/kg p.o., exhibited a significant inhibitory effect on the increased levels of bradykinin released into the pouch fluid of kaolin-induced inflammation in rats. In the kaolin-induced writhing response in mice, which is shown to be mainly dependent on the action of bradykinin, T-614 reduced not only the writhing frequency but also the peritoneal levels of bradykinin in a dose-dependent manner. Whereas, in the zymosan-induced writhing response in which prostaglandin I2 (PGI2) is shown to be an important mediator, it did not exert an obvious inhibition on either writhing responses or peritoneal PGI2 levels at a highest dose of 100 mg/kg. T-614 did not inhibit the activities of serine proteases, such as trypsin, thrombin, kallikrein and plasmin. Furthermore, it did not affect the kinin-forming enzymes of rat plasma in vitro. The above results suggest that the analgesic effects of T-614 may be partly mediated by the inhibition of bradykinin release in the local inflamed tissue.

Analgesia↗
PubMed1992

[Sphenoid sinus aspergillosis presenting abducens nerve palsy and visual field impairment; a case report].

A case of sphenoid sinus aspergillosis presenting abducens nerve palsy and visual field impairment is reported. A 73-year-old woman visited our hospital with the complaint of head heaviness on the 27th of March, 1989. Although results of neurological examinations were normal, craniogram revealed the destruction of the clivus, and CT scan and MRI showed a mass lesion, which was thought to be a mucocele in the sphenoid sinus. On the 1st of September, she developed right abducens nerve palsy and visual field impairment. MRI performed on the same day showed an enlargement of the mass lesion in the sphenoid sinus. In order to decompress the involved cranial nerves, her sphenoid sinus was explored on the 22nd of September. The sphenoid sinus was filled with purulent fluid and yellowish mass. Histopathological examination revealed colonies of aspergillus fumigatus. Fluconazole, a new antifungal drug, was given for 34 days postoperatively. The right abducens nerve palsy and the visual field impairment gradually improved along with a reduction of the mass lesion in her sphenoid sinus. Sphenoid sinus aspergillosis is a rare disease. Its diagnosis is difficult. However, MRI can show a specific low signal intensity in T2-weighted image. Also in our case, MRI on the first admission showed a definite low signal intensity in some parts of the lesion, which exhibited a high intensity later on during the second administration, probably due to a qualitative change. To our knowledge, only 33 such cases have been previously reported. Intracranial involvement occasionally occurs in this disease. In its early stage, cranial nerve palsies are caused by nerve compression or invasion by this disease.(ABSTRACT TRUNCATED AT 250 WORDS)

Abducens Nerve↗
PubMed1992

[Analysis of lymphocyte surface markers in childhood acute lymphoblastic leukemia during maintenance chemotherapy].

In this study we investigated T-cell subsets, HLA-DR and IL2 receptor (IL2R, CD25) expression on T-cells in peripheral blood lymphocytes from the patients with childhood acute leukemia undergoing maintenance chemotherapy with 6-MP and MTX, and compared them with those in the healthy controls. There were decrease of relative number of CD4 positive (CD4+) cells and the increase of relative number of CD8 positive (CD8+) cells in comparison with those in controls; thus the ratio of CD4+ cells to CD 8+ cells decreased. HLA-DR and IL2R expression on T-cells increased in the patients. Increased HLA-DR expression was detected on both CD4+ cells and CD8+ cells, but the increased IL2R expression was related only to CD4+ cells. IL2R expression in the patients with side effects was higher than in controls. Methotrexate (MTX)-induced increase of HLA-DR and IL2R expression was observed in 2 of every 3 patients undergoing intensive chemotherapy.

Antigens, Surface↗
PubMed1992

Establishment of a monocytoid cell line (DOP-M1) from an infant with acute monocytic leukemia.

A monocytoid cell line (DOP-M1) was established from mononuclear cells separated from the cerebrospinal fluid of a 1-year-old girl with acute monoblastic leukemia (AMoL) (French-American-British; FAB-M5a). Judged by morphological, cytochemical, and immunological criteria, the DOP-M1 cells showed immature monocytoid characteristics. They were positive for alpha-naphthyl butyrate esterase, the expression of which was inhibited by NaF, and for myeloperoxidase (MPO). Positive MPO findings in nuclear envelope were detectable by electron microscopy. The cell surface was positive for CD15, CD33, and CDw65, but negative for CD4, CD14, and HLA-DR. HLA-DR expression was detected after treatment with IFN-gamma. Chromosome analysis of DOP-M1 cells revealed 47,X,-X,-13,+19,+20,+mar. Our established cell line, DOP-M1 appears to be a cell line which will be a useful tool for studying the phenotype, morphology, and function of monocytoid cells.

Female↗