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Biomedical subjects

M Eguchi

Publications and source records attributed to M Eguchi.

At least 253 records · Page 14Linked to original sources
PubMed1979

Hurler-Scheie phenotype: a report of two pairs of inbred sibs.

Four cases from two families with dermatan sulfate mucopolysacchariduria who lack alpha-L-iduronidase in peripheral leukocytes are described. The clinical and roentgenographic features of these cases represent an intermediate phenotype between Hurler's syndrome and Scheie's syndrome, and both parents in each family are first cousins. In the presence of parental consanguinity, a phenotypic variation or a third mutant allele at the iduronidase locus seems to be a more reasonable explanation for these cases than a genetic compound.

Adult↗
PubMed1979

Subcellular localization of complex carbohydrates in rat macrophages and monocytes.

Methods for visualization of complex carbohydrates ultrastructurally were employed to study specific organelles of the rat monocyte and macrophage. Vicinal glycols of glycoconjugates were demonstrated with the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) postembedding sequence and acid groups were delineated by the dialyzed iron (DI) and high iron diamine (HID) preembedding techniques. Lysosomal bodies were generally found reactive with all three methods, although those of monocytes from the bone marrow and peripheral blood were notably lacking in acidic groups. The Golgi complex was consistently PA-TCH-SP-reactive, as were associated vesicles and occasional cisternal expansions, possibly related to GERL. Numerous cytoplasmic vesicles and small granulated structures and cisternae of the rough endoplasmic reticulum were also PA-TCH-SP-reactive.

Animals↗
PubMed1979

Peroxisomes of rat peritoneal macrophages during phagocytosis.

The peroxisomes of resident macrophages in the rat peritoneal cavity were examined during the phagocytosis of latex microbeads, employing the akaline diaminobenzidine (DAB) technique. Peroxisomes generally were located in close proximity to phagosomes and were often observed in a process of apparent fusion with phagosomes. Cytochemical evidence was also obtained for discharge of catalase from peroxisomes to phagosomes. The profiles indicating fusion were observed after 10 minutes of incubation with microbeads. The number of peroxisomes was increased in macrophage profiles examined 30 minutes after exposure to microbeads. Acid phosphatase was localized in small vesicles that were distinct from peroxisomes, and peroxidase was not demonstrable in peroxisomes. A method for ultrastructural localization of periodate reactive complex carbohydrate demonstrated glycoproteins in numerous small vesicles or granules, some of which possibly represented peroxisomers. The possible function of peroxisomes during phagocytosis in rat peritoneal macrophages is considered.

Acid Phosphatase↗
PubMed1979

Ultrastructural localization of complex carbohydrates in rat monocytes and peritoneal and alveolar macrophages.

Complex carbohydrates are demonstrated in lysosomes and other organelles of rat monocytes and macrophages by means of ultrastructural morphochemical techniques for localizing acidic groups and vicinal glycols that are characteristic of glycoconjugates. The presence of glycoconjugates in various cell organelles differ in monocytes and macrophages from different sites. Bone marrow and blood monocytes contained glycoproteins with periodate-reactive vicinal glycols, as shown by the periodate-thiocarbohydrazide-silver proteinate sequence in lysosomes, a population of small vesicular structures, and Golgi lamellae. However, these cells contained no dialyzed iron or high iron diamine reactivity indicative of acidic groups. Alveolar macrophages were similarly periodate-thiocarbohydrazide-silver proteinate-reactive, and contained a variable degree of dialyzed iron and high iron diamine reactivity demonstrative of acid mucosubstances in lysosomes. Peritoneal macrophages, on the other hand, exhibited highly reactive lysosomes with all three techniques, and revealed a population of periodate-thiocarbohydrazide-silver proteinate-positive vesicles not previously recognized and not readily discernable by other means. These cells also displayed periodate-thiocarbohydrazide-silver proteinate staining in large cisternal elements, possibly related to Golgi-endoplasmic reticulum-lysosome (GERL) and in other Golgi complex-related elements, as well as in cisternae of rough endoplasmic reticulum.

Animals↗
PubMed1979

Idiopathic sideroblastic anemia: presence of sideroblastic changes in the erythropoietic precursors cultured from peripheral blood.

Peripheral blood mononuclear cells from five patients with idiopathic sideroblastic anemia were examined in clonal culture for circulating erythropoietic precursors. Three of these patients had relatively mild anemia and revealed significant burst formation. Two patients who had severe anemia and required regular blood transfusions showed severe diminution or absence of circulating precursors. These results could suggest that the number of circulating erythropoietic precursors reflects effective erythropoiesis in the marrow. Ultrastructural morphology and ultrastructural Prussian-blue staining of the bursts from a patient revealed the characteristic mitochondrial deposition of ferrugionous material in pathologic normoblasts. Peripheral blood clonal cell cultures would, therefore, appear to be a potentially useful tool for analysis of biochemical abnormalities in idiopathic sideroblastic anemia.

Aged↗