Biomedical subjects

M E Stromski

Publications and source records attributed to M E Stromski.

21 records · Page 2Linked to original sources

PubMed1986

Postischemic ATP-MgCl2 provides precursors for resynthesis of cellular ATP in rats.

Postischemic administration of ATP-MgCl2 is known to enhance recovery of renal function and accelerate the restitution of cellular ATP levels. To differentiate between a direct and indirect effect of the exogenous nucleotide, rats were subjected to 45 min of bilateral renal ischemia and were infused with either ATP-MgCl2, AMP-MgCl2, or normal saline. The immediate recovery of the cellular ATP was similar in all three groups of animals, whereas the subsequent recovery was accelerated by the infusion of either nucleotide. Since ATP-MgCl2 and AMP-MgCl2 produced similar results, this study provides evidence that exogenous ATP may act by providing precursors for the resynthesis of the cellular adenine nucleotide pool rather than being a direct source of energy.

Adenosine Triphosphate↗

PubMed1983

High-resolution 1H nuclear magnetic resonance study of cerebral hypoxia in vivo.

1H NMR spectra at 360.13 MHz were obtained of the rat brain in vivo by using a surface coil placed over the skull. Resonances of numerous metabolites were identified by comparison with the 1H NMR spectra of excised rat brain tissue and acid extracts of the tissue. Changes in cerebral lactate levels resulting from the administration of gas mixtures low in oxygen were monitored in the in vivo brain spectra with a time resolution of 2.3 min. The electroencephalogram and electrocardiogram were recorded simultaneously during the NMR experiment. Reversibility of the hypoxic response was documented when, upon oxygen administration, cerebral lactate returned to its prehypoxic level. These experiments demonstrate the applicability of high-resolution 1H NMR to monitor pathophysiology of brain metabolism in real time.

Animals↗

PubMedDate not supplied

Methylamines and polyols in kidney, urinary bladder, urine, liver, brain, and plasma. An analysis using 1H nuclear magnetic resonance spectroscopy.

Methylamines and polyols are known to behave as organic osmolytes in the adaptation of many cells to hyperosmolar conditions. Using 1H nuclear magnetic resonance spectroscopy to analyze perchloric acid extracts we have examined several tissues in the rat for the presence of these compounds. Methylamines such as glycerophosphorylcholine, choline and betaine were observed in the renal inner medulla, urinary bladder, urine, liver, brain, and plasma. Myoinositol was relatively abundant in the renal inner medulla and brain whereas sorbitol was detected only in the inner medulla. A variety of unidentified compounds was also detected in each tissue. Although these methylamines and polyols are known to respond to osmotic changes in the renal inner medulla, their responses in other tissues remain to be investigated.

Animals↗