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Biomedical subjects

M E Pinto

Publications and source records attributed to M E Pinto.

At least 55 records · Page 3Linked to original sources
PubMed1975

The effect of antibody and complement on perfused hearts: a model for the study of xenograft rejection phenomenon.

An in vitro heart perfusion system was developed to study the effect of antibody and complement on hearts in vitro, as a model to quantitatively evaluate factors involved in xenograft rejection. Both guinea pig and rabbit hearts were tested with various reagents. Perfusion of hearts with normal autologous or "homologous" sera did not induce rejection, while "heterologous" sera, normal or immune, arrested cardiac function. Either the inactivation of certain complement components or the absence of antibodies against heart tissue prevented rejection as determined by lack of change in electrocardiogram and alterations in ultrastructure. Antibody alone had no effect. A lethal effect of C6-deficient rabbit serum on guinea pig hearts was prevented by 2 antihistamines, Chlortripolon and Benadryl.

Animals↗
PubMedDate not supplied

In vitro antibacterial activity of trospectomycin (U-63,366F) against anaerobic bacteria and aerobic gram-positive cocci in Chile.

The in vitro activity of trospectomycin sulfate was compared with those of several antimicrobials, against 301 anaerobic bacteria and 613 aerobic Gram-positive cocci. Trospectomycin was about 4- to 32-fold more active than was spectinomycin. Trospectomycin exhibited consistently good activity against all Bacteroides fragilis group isolates, except Bacteroides vulgatus, and against all other anaerobes comparable or higher to that of clindamycin. The trospectomycin's activity was most similar to that of vancomycin, even against methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis.

Anti-Bacterial Agents↗
PubMedDate not supplied

V3 peptide binding pattern and HIV-1 transmission route in Rio de Janeiro.

To characterize antibody binding to a panel of V3 loop peptides representing diverse HIV-1 neutralization epitopes, 149 HIV-1 infected individuals from Rio de Janeiro (RJ) were investigated. Results were analyzed with respect to risk factors for infection and other epidemiological and clinical data. Peptide reactivity was not associated with sex, clinical status, CD4 counts, antigenemia or beta 2-microglobulin serum level. A segregation of peptide reactivity according to route of infection was encountered. This finding suggests that more then one viral strain may be circulating in RJ, in subjects with different risk factors for HIV-1 infection. An investigation of prevalent HIV-1 genotypes, serotypes and immunotypes may be of importance for the design and selection of potential vaccines to be used in Brazil as well as for the selection of populations to be included in future vaccine efficacy trials.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

[Bacterial isolation in infants hospitalized for acute diarrhea].

Bacteria were investigated in stools of 156 children under two years of age admitted to the pediatric wards of a general hospital at the western metropolitan area of Santiago, Chile, because of acute diarrhea with only one stool sample. A known pathogenic agent was isolated from 115 cases (73.7%), this being a bacteria in 87/115 (75.6%). Most prevalent microorganisms were Enteropathogenic Escherichia coli (EPEC). (65.5%), specially serogroups 0111, 0119 and 055, Enterotoxigenic Escherichia coli (ETEC) (18.4%), Campylobacter yeyuni (13.8%), Salmonellae (9.2%) and Shigellae (6.9%). Invasive Escherichia coli (EIEC) and Aeromonas hydrophila were observed in only one case. Yersinia enterocolitica was not isolated. Age was under one year in 92% of patients and 83% were normally or slightly under nourished. Fecal leucocytes were abnormally increased (greater than 5 per high power field) in 41.4% of positive bacterial isolates, in 83% of children with Shigellae, in 50% of those with EPEC and Salmonellae and in 25% of the Campylobacter yeyuni group.

Acute Disease↗