Search PubMed⌕ Search

Biomedical subjects

M E Phillips

Publications and source records attributed to M E Phillips.

97 records · Page 6Linked to original sources
PubMedDate not supplied

Neoplastic vs inflammatory meningeal enhancement with Gd-DTPA.

We studied 40 consecutive patients clinically suspected of having meningeal disease, in whom both Gd-diethylenetriamine pentaacetic acid (DTPA)-enhanced magnetic resonance (MR) examinations and CSF or surgical pathologic samples were obtained. The MR examinations were retrospectively reviewed for the presence of abnormal meningeal enhancement, as well as pattern and site of enhancement. The MR findings correctly correlated with pathology results in 15 of 19 patients with suspected tumor involvement (including one true negative) and in 18 of 21 patients with suspected inflammatory meningeal conditions (including 2 true negatives). Pial enhancement was demonstrated in 62% (10 of 16) of patients with meningitis and in 29% (4 of 14) of patients with meningeal neoplastic involvement. A diffuse meningeal distribution was commonly present with inflammatory conditions (69%, 11 of 16). A nodular appearance was seen in two patients with tumor involvement of the meninges as well as in a single case of sarcoidosis but was not seen with infectious meningitis. Our data suggest that Gd-DTPA-enhanced MR imaging is sensitive to the presence of meningeal pathology but is nonspecific as to disease entity. Recognition of certain MR patterns in conjunction with clinical presentation may improve diagnosis and management of patients with suspected meningeal disease.

Contrast Media↗
PubMedDate not supplied

The substitution of glucose-antiglucose oxidase complex (GAG) for peroxidase-antiperoxidase (PAP) in immunohistochemical studies of skin.

The substitution of glucose-antiglucose oxidase complex (GAG) for peroxidase antiperoxidase (PAP) in the Sternberger three-layer unlabeled antibody method avoids the problems associated with the use of the chromogen 3,3,diaminobenzidine (DAB). The blue final reaction product obtained using the GAG complex is not obscured by melanin granules, which is often the case when using the PAP-DAB combination. The replacement of PAP with GAG complexes facilitates the detection of melanocytes in melanoma and blue nevi. The absence of endogenous glucose oxidase in mammals provides for greater image contrast, which is not possible using alternative chromogens and immunoperoxidase.

Glucose Oxidase↗