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Biomedical subjects

M E Haynes

Publications and source records attributed to M E Haynes.

12 recordsLinked to original sources
PubMed1980

Metabolic effects of exercise training in hemodialysis patients.

The effects of 9 +/- 6 months of exercise training on lipid and carbohydrate metabolism were studied in six hemodialysis patients. Training lowered triglyceride levels 39 +/- 25% (P less than 0.02) and increased plasma high-density lipoprotein cholesterol levels 23 +/- 22% (before, 26 +/- 5 mg/dl; after, 31 +/- 8 mg/dl; P less than 0.05). There was a 23% improvement in glucose tolerance (P less than 0.01) and a 40% reduction in hyperinsulinism (P less than 0.01) with no significant changes in body weight or diet. There was a 25 +/- 8% increase in hematocrits (before, 22 +/- 2%; after, 27 +/- 2%, P less than 0.01) and a 29 +/- 2% rise in hemoglobin concentrations (before, 7.0 +/- 0.8 g/dl; after, 9.0 +/- 0.6 g/dl, P less than 0.04) in five patients. In addition, during training antihypertensive medications could be reduced in three patients with maintenance of normal blood pressure. The improvements in lipid and carbohydrate metabolism diminished when two patients stopped training. These results suggest that physical training can improve some of the metabolic abnormalities observed in hemodialysis patients and could be important as a therapeutic modality.

Adult↗
PubMed1979

Electron microscopy of skin and peripheral blood lymphocytes in infantile (Santavuori) neuronal ceroid lipofuscinosis.

Skin punch biopsies and peripheral blood lymphocyte preparations from two siblings with early infantile (Santavuori) neuronal ceroid lipofuscinosis have been examined by electron microscopy. In both cases characteristic osmiophilic inclusion bodies were found in various cells in the skin and in lymphocytes. In one case, lymphocyte inclusions were detected before the onset of any symptoms as a result of a family study. This indicates the possibility of screening lymphocytes of siblings of affected patients as a result of a family study. This indicates the possibility of screening lymphocytes of siblings of affected patients with a view to early detection of the disorder.

Ceroid↗
PubMed1976

Electron-microscope observations on cell nuclei in various tissues of a teleost fish: the nucleolus-associated monolayer of chromatin structural units.

Observations on stain uptake by thin sections through condensed interphase chromosomes in cells from epithelial and muscle tissue in kidney and intestine, and also in fibroblasts, show a distribution into DNA-rich and DNA-poor phases similar to that already described in cells from the connective tissue blood. In all the nuclei the nucleolus, when adjacent to the nuclear envelope, is separated from the inner membrane by a monolayer of chromatin structural units, similar to the monolayer enclosed on both sides by nuclear envelope, previously described in a wide variety of organisms. The data provide further support for the hypothesis that the condensed interphase chromosomes in eukaryotes are characterized by essentially similar structural units folded to form similar patterns. This hypothesis, regarding the higher order units, is consistent with data of others which show that histones and DNA fold to form similar repeating subunits in chromatin, irrespective of the base sequence in the DNA and the origin of the histones.

Animals↗
PubMed1975

Light- and electron-microscope observations on certain leukocytes in a teleost fish and a comparison of the envelope-limited monolayers of chromatin structural units in different species.

Previously it was shown that the nuclear envelope-limited sheets of chromatin, monolayers of nucleoprotein structural units, are present in blood cells from 4 classes of vertebrates. Now we show that sheets of similar width are present in certain leukocytes of a fifth class, a teleost fish. We describe the fine structure of leukocytes in peripheral blood and in the main haematopoietic organ, kidney. We also examined the granulocytes of connective tissue in intestine. By May-Grunwald-Giemsa staining and electron microscopy heterophilic granulocytes, cosinophils but no basophils could be recognized in peripheral blood and kidney. Problems in classification of the cells are discussed. In one group (A) of 5 fish, sheets occurred at a frequency of roughly 1% in heterophilic (type 1) granulocytes and lymphocytes from peripheral blood. No sheets were found in a second group (B) of 5 fish. Kidney and intestine were examined in some fish from both groups and no sheets were present. In an atypical group (C) sheets were found in the eosinophilic (type 2) granulocytes from peripheral blood of one fish and in lymphocytes from connective tissue of intestine in another. Sheets were usually associated with nuclei of irregular shape and their width averaged 36 nm. We tabulate data from other workers on occurence and width of sheets. They are found in all the main classes of tissue in mammals, namely blood and other connective tissues, in epithelial, nervous, germinal tissue and muscle, as well as in invertebrate and certain plants. Their nearly constant width, average value 35 nm, provides very convincing evidence for the hypothesis that the molecules of DNA and protein are organized into the same fundamental structural units, irrespective of species. We discuss the variable incidence of sheets among different cell types and the factors which might determine this.

Animals↗
PubMed1972

Cytoplasmic particles in hepatocytes of patients with Australia antigen-positive liver disease.

An electron microscope study was made of liver biopsy material obtained during life or shortly after death from 11 patients. In three of them, where serum was positive for Australia antigen (two had serum hepatitis and one active chronic hepatitis) the hepatocytes contained characteristic particles within membrane-bound cytoplasmic vesicles. The appearance of these particles was very similar to that of the Australia antigen particles found in the serum. Two sizes of cytoplasmic particles were observed, with average diameters of about 26 and 46 nm. Particles of both sizes often had a membrane-like outer component and a moderately electron-dense inner component. They differed in both size and structure from the mainly intranuclear particles described by previous authors and which were not found in the present patients. The nature of the vesicles containing the particles was not apparent.

Adult↗
PubMedDate not supplied

How to conduct quality meetings.

Meetings are commonplace in modern America with the average executive spending 17 hours a week in session. In addition to the working-hours meetings, nearly everyone participates in meetings of professional societies, civic associations, service clubs, and church groups. With meetings occurring so frequently one could expect them to be a source of satisfaction and accomplishment. However, this often is not the case. This article will help to improve the quality of your meetings, whether you are a leader or a participant. It is based on the premise that an effective meeting achieves its objective within a reasonable amount of time. The article is divided into four sections: planning meetings, conducting meetings, improving meetings, and a conclusion.

Administrative Personnel↗