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Biomedical subjects

M E Cruz

Publications and source records attributed to M E Cruz.

43 records · Page 3Linked to original sources
PubMed1985

Pilot study to detect neurologic disease in Ecuador among a population with a high prevalence of endemic goiter.

A census, uniform screening questionnaire, and simple screening neurologic examination were administered in a door-to-door survey to residents of Quiroga, Ecuador, a rural community in the Andes Mountains. The screening procedures had been pretested to assure a high level of sensitivity for detecting children and adults with major neurologic disease. A total of 1,113 participated in the study. Of these, 399 had responses or findings suggesting the presence of neurologic disease. These individuals were then examined by a neurologist, who used fixed diagnostic criteria. The prevalence ratios (per 1,000) for the most common neurologic conditions identified in this survey are: recurrent/persistent severe headache = 68.3, and epilepsy = 17.1.

Adolescent↗
PubMedDate not supplied

Biochemical characterization of an L-asparaginase bioconjugate.

In this work is characterized a bioconjugate of L-asparaginase, obtained by linkage of palmitic acid chains to the native enzyme in the presence of substrate as a protein protective molecule. Comparisons between isoelectric points, hydrophobicity, pH, and temperature profiles for the bioconjugate and the native enzyme were performed. A shift of pI from 5.03 to 4.58 was observed after conjugation. The modified enzyme evidences a 10-fold increase of the hydrophobicity. A small shift from 7.5 to 7 of the pH for maximal catalytic activity and a 5 degree C increase of temperature for maximal activity were observed with conjugation. Stability studies in human serum and on storage evidence similar behaviors for both bioconjugate and native enzyme. The retention of catalytic activity of the bioconjugate is dependent on the presence of micelles. The bioconjugate evidenced 65% retention of activity when catalytic activity was assayed without a surfactant and 98-100% retention of activity when catalytic activity was assayed in the presence of surfactant micelles. The kinetic characteristics of the bioconjugate and of the native enzyme, in micelles of different hydrophobicities, were compared. The Michaelis constant of native enzyme is 0.030 mM, independent of the surfactant, and the Michaelis constant of the bioconjugate varies with the surfactant, from 0.036 to 0.046 mM.

Asparaginase↗
PubMedDate not supplied

Low blood glucose levels and other complications during growth hormone supplementation in sepsis.

Blood glucose levels in the high normal range or even moderate hyperglycemia is the expected profile in septic postoperative patients receiving high-calorie enteral alimentation. The addition of growth hormone as an anabolic agent should additionally reinforce this tendency. In a cancer patient undergoing partial gastrectomy with lymphadenectomy and suffering from postoperative subphrenic abscess and prolonged sepsis, tube feeding (38.3 kcal/kg/day) and growth hormone (0.17 IU/kg/day) were simultaneously administered for 25 days. Blood glucose levels were in the lower limits of the normal range before growth hormone introduction, and continued with a similar tendency during most of the therapeutic period. Two additional complications, namely heart arrest and peripheral edema, were documented during the same period. It is concluded that sepsis was the most likely mechanism for low glucose values, and that high-calorie enteral diet and growth hormone supplementation did not prevent that result. It is uncertain whether heart arrest was due to the drug, but its association with peripheral edema is well documented in clinical series.

Aged↗