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Biomedical subjects

M Donner

Publications and source records attributed to M Donner.

At least 91 records · Page 5Linked to original sources
PubMed1979

[Differential interaction of 2,4,6-trinitrobenzenesulfonic (TNBS) and 2,4-dinitrobenzenesulfonic (DNBS) acids on mouse lymphocytes].

Modifications of lymphoid cells with dinitro-2,4 phenylsulfonic acid (DNBS) or trinitro-2,4,6 phenylsulfonic acid (TNBS) have been studied. TNBS action always produces an electrophoretic mobility increase in relation with the amount of amino-groups, according to the cell type. DNBS action produces an electrophoretic mobility increase for B cells of spleen and a decrease for T cells of spleen and thymic cells. The hydrophobic fluorescent probe 1-anilino-8-naphthalene sulphonate used to study conformational changes of cells revealed a slight fluorescence decrease for TNP-modified cells and an important fluorescence increase for DNP-modified cells.

Animals↗
PubMed1979

Incidence and aetiology of infantile spasms from 1960 to 1976: a population study in Finland.

This series of cases of infantile spasms comprised 107 children born between 1960 and 1976 in the county of Uusimaa, Finland. The incidence of the syndrome was 0.38 to 0.42/1000 live births and has remained almost unchanged during those years, although there have been changes in the size of different aetiological groups. The importance of prenatal and perinatal factors, and especially of neonatal hypoglycaemia, as causal factors has decreased, whereas the numbers of cases due to infections and malformations has increased. Compared with investigations in other countries, this Finnish series differs in the incidence of familial cases and congenital cytomegalovirus infection, and especially in the higher incidence of neonatal hypoglycaemia and the lower incidence of tuberous sclerosis.

Birth Injuries↗
PubMed1979

Single cell analysis of changes in electrokinetic properties of a growing ascitic tumor.

An ascitic tumor (SEWA) induced by polyoma virus in A.SW mice was analyzed in vivo as well as in vitro with regard to the electrophoretic mobility (EPM) which may be considered as a reliable criterion of surface charge. After the i.p. transplantation of 10(5) cells, the EPM decreased up to 14th day. Then, the mobility gradually increased with the age of the tumor. In the first phase of tumor growth, we have considered the possibility that immunoglobulin cell coating may be responsible for the decrease in EPM. In the late phase of SEWA growth, the progressive increase in EPM might be due to a rearrangement of sialic acids on the outer part of the cell membrane.

Animals↗
PubMed1979

Characterization of the human peripheral effector cells mediating antibody dependent cellular cytotoxicity against allogenic cells.

The effector cell populations in human peripheral blood responsible for antibody-dependent cellular cytotoxicity against allogenic cells coated with HLA polyspecific antibodies were investigated using several separation techniques including preparative electrophoresis. Electrophoresis produced a marked effector cells enrichment in a range of 2--5 fractions which exhibited an intermediary electrophoretic mobility. Monocytic cells do not contribute an effector mechanism but minor subsets of polymorphonuclear cells and nylon wool non-adherent non-phagocytic lymphocytes displayed ADCC. Both effector cell populations were found to exhibit a similar electrical charge of cell surface centered around -1.05 micrometer . sec-1 V-1 cm. These observations provided a precise biophysical basis for the identification of effector cells in ADCC.

Adult↗
PubMed1978

Occurrence of risk factors in newborn infants. A study of 22359 consecutive cases.

All the 22359 deliveries in 1971-1974 at the Institute of Midwifery, Helsinki, were analyzed for risk factors. 1196 infants (5.35%) had one or more risk factor at birth, the most common being an Apgar score of 6 or less at 5 or 15 minutes, hyperbilirubinaemia, and a birth weight of 2000 g or below. The other risk factors registered were neurological symptoms, respiratory difficulties, hypoglycaemia, newborn infants of diabetic mothers and cases with sepsis. 124 infants in the risk group died during the first week. This was 83% of the total neonatal mortality. Except for hyperbilirubinaemia, which was less frequent in 1974, there was no marked change in the composition of the risk group from 1971 to 1974.

Apgar Score↗
PubMed1977

Fatal measles encephalopathy with retinopathy during cytotoxic chemotherapy.

A 14-year-old boy receiving post-operative cytotoxic chemotherapy for a testicular rhabdomyosarcoma developed a fatal encephalopathy associated with retinal changes 2 months after an episode of acute measles. Post-mortem histological examination showed intranuclear inclusion bodies in the neurons and glial cells, but inflammatory cell infiltrations were absent. Electron-microscopic and immunofluorescent studies revealed intracellular masses of paramyxovirus nucleocapsid-like structures, which had the morphological and antigenic properties of measles virus. Recent reports have emphasized the possibility of occurrence of a similar encephalopathy in treated childhood leukemia. It is evident, however, that this potentially fatal complication must be borne in mind when measles is contracted during any form of cytotoxic treatment or immunosuppression. Retinal changes may be of value for the diagnosis during life. We propose the designation "measles encephalopathy during immunosuppression" (MEI) for this condition.

Adolescent↗
PubMed1976

Modifications of the thymocyte membrane during redistribution of concanavalin A receptors.

The modifications of the thymocyte membrane which are induced by concanavalin A (Con A) were studied by means of the variation of electrophoretic mobility which the lectin induces on the cell. The electrophoretic mobility of thymocytes is increased when Con A is used under conditions expected to induce a cap formation and the subsequent endocytosis. This increase persists as long as the lectin is present in the medium and disappears as soon as the lectin is eliminated. The redistribution of the Con A receptors into spots and caps may partially explain this electrical modification. The ionized groups of the thymocyte membrane are drastically modified during these phenomena with: 1) a diminution by 61% in the density of the sialic carboxyl groups, 2) a decrease of 40% in the density of phosphate groups and of 60% in the density of amino groups, 3) a 20 times higher density of unidentified negatively charged groups. The electrophoretic mobility of normal human blood lymphocytes is similarly increased by Con A. A marginal difference exists in the shape of the dose response curves obtained when normal and cancerous thymocytes react with increasing doses of Con A. No measurable electrical modification was observed during redistribution of H-2 and Thy-1.2 antigens on EL4 cells. Some experiments suggest a possible correlation between the mitogenicity of the lectin and a measurable modification of the cell electrophoretic mobility.

Animals↗
PubMed1976

Creatine kinase isoenzymes in neuromuscular diseases.

Determination of the creatine kinase isoenzyme pattern in 62 biopsy samples obtained from patients with neuromuscular disease revealed changes mainly in Duchenne muscular dystrophy. The BB isoenzyme was detected in 10 out of 17 cases with Duchenne muscular dystrophy and the relative amount of MB+BB isoenzyme was significantly increased in this group (P less than 0.005). In serum the MB isoenzyme was detected in all 28 cases with progressive muscular dystrophy and frequently also in other neuromuscular diseases. Among 152 control samples the MB isoenzyme was detected only in 2 cases. It is suggested that the finding of MB isoenzyme in the serum with normal or only slightly elevated total CK activity may be a further proof of neuromuscular disorder, but the finding is not specific for any particular disease.

Adult↗