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M Dong

Publications and source records attributed to M Dong.

At least 37 records · Page 2Linked to original sources
PubMed2001

Increased nitric oxide in peritoneal fluid from women with idiopathic infertility and endometriosis.

OBJECTIVE: To verify whether nitric oxide in peritoneal fluid is associated with endometriosis and infertility. STUDY DESIGN: Twenty-five women with idiopathic infertility and 38 with endometriosis were recruited, and 18 cases of uterine myomata and 2 cases of ovarian cyst served as controls. Peritoneal fluid samples were aspirated from the pouch of Douglas during laparoscopy or laparotomy. Metabolites of nitric oxide (nitrite and nitrate) in peritoneal fluid were determined by a method using nitrate reductase and the Griess reaction. RESULTS: Peritoneal concentrations of nitrate/nitrite in both infertile women (42.02 +/- 12.98 mmol/L) and patients with endometriosis (41.75 +/- 16.42 mmol/L) were significantly higher than that in controls (33.96 +/- 13.07, P < .05 for both). No significant difference in peritoneal nitrate/nitrite level was found between infertile women and patients with endometriosis (P > .5). Peritoneal levels of nitrate/nitrite were comparable among patients with endometriosis at different stages (P > .5). Patients with endometriosis had more peritoneal fluid than controls and idiopathic infertile women, while controls and idiopathic infertile women had comparable amounts of peritoneal fluid. CONCLUSION: An increased peritoneal level of nitric oxide is a common alteration in endometriosis, endometriosis-associated infertility and idiopathic infertility and may be associated with the pathogenesis of these diseases.

Adult↗
PubMed2001

Influence of growth hormone-insulin-like growth factor I axis on normal pregnancy.

OBJECTIVE: To investigate the role of growth hormone-insulin-like growth factor I (IGF-I) axis in normal pregnancy. METHODS: Totally, 116 normal pregnant women were recruited from January 1997 to June 1998, with 20 normal nonpregnant women as controls. Maternal growth hormone (GH) and IGF-I concentrations were assayed by RIA and enzyme-linked immunosorbent assay, respectively. RESULTS: Maternal serum levels of GH increased throughout gestation, reached a peak at 25 weeks of pregnancy and remained fairly high (chi 2 = 40.458, P < 0.0001). There was a significant difference between samples at 5-9 week gestational age and the controls (3.45 micrograms/L vs 1.61 micrograms/L, P < 0.05). The maternal serum levels of IGF-I increased rapidly throughout gestation from 29-week gestation and reached a peak of 188.86 micrograms/L at term delivery (chi 2 = 50.224, P < 0.0001). CONCLUSIONS: Maternal GH levels increased progressively throughout gestation, which correlated with fetal growth. Maternal GH may regulate nutrition supply among mother, placenta and the fetus and play an important role in transporting nutritional substrates by the placenta. The maternal IGF-I in the third trimester may promote fetal growth and placental functions.

Adult↗
PubMed2001

[Placental expression of epidermal growth factor receptor in pregnancy induced hypertension].

OBJECTIVE: To clarify the expression of epidermal growth factor receptor (EGFR) in placenta from women with pregnancy induced hypertension (PIH). METHODS: EGFR of placenta was determined by immunohistological method and compared between normal pregnancies (34 cases) and pregnancies accompanied by PIH (30 cases). RESULTS: Positive immunostain of EGFR was located on cytoplasm, and membrane of syncytiotrophoblast and a small part of cytotrophoblast. Compared with that of normal pregnancy, the expression of EGFR in placenta from pregnancies complicated with PIH decreased significantly (P < 0.05). No significant differences of EGFR expression in placenta were found among women with mild, moderate and severe PIH (P > 0.5). No significant correlation was found between expression of EGFR and neonatal birthweight, placental weight (P > 0.01, for both). CONCLUSION: Decreased expression of placental EGFR was found in women with PIH, and that may play a role in the pathogenesis of PIH.

Adult↗
PubMed2001

[Study on the relation between the cell cycle regulators and laryngeal carcinogenesis].

OBJECTIVE: To investigate the clinicopathological significance of the Cyclin E, p21WAF1/CIP1 and p53 expression in laryngeal carcinogenesis. METHOD: The expression of Cyclin E, p21WAF1/CIP1 and p53 was examined by using immunohistochemical method in normal epithelia (20), dysplastic epithelia (40) and laryngeal cancers (60). RESULT: 1. In normal epithelium, dysplastic squamous epithelium and in squamous cell carcinoma of the larynx, the positive immunostaining rate of Cyclin E protein was 5.0%(1/20), 20.0%(8/40) and 45.0%(27/60) respectively (P < 0.001), and p21WAF1/CIP1 protein was 95.0%(19/20), 75.0%(30/40) and 63.3%(38/60) respectively (P < 0.01), while the rate of p53 was 0, 30.0%(12/40) and 61.7%(37/60)(P < 0.001) respectively. 2. p21WAF1/CIP1 positive expression in highly, moderately and poorly differentiated carcinomas were 76.2%(16/21), 66.5%(19/29) and 30.0%(3/10) respectively (P < 0.05), p21WAF1/CIP1 protein expression was significantly associated with tumour cell differentiation. 3. The positive expression of Cyclin E closely related with the positive expression of p53 (P < 0.05). No correlation was found between p21WAF1/CIP1 expression and p53 expression. CONCLUSION: 1. Cyclin E and p53 could be an early molecular event in the carcinogenesis of larynx. 2. p21WAF1/CIP1 expression is associated with tumour cell differentiation. 3. The mutual mechanism of cell cycle regulators (Cyclin E, p21WAF1/CIP1 and p53) play an important role in the laryngeal carcinoma.

Adult↗
PubMed2001

[Isolation and identification of steroidal saponins from Dioscorea panthaica Prain et Burkill].

AIM: To investigate the chemical constituents of the rhizomes of Dioscorea panthaica Prain et Burkill and look for new active compounds. METHODS: The compounds were isolated with silica gel and HPLC, and their structures were elucidated on the basis of chemical evidences and spectral analysis (IR, ESI-MS, 1HNMR, 13CNMR, DEPT, 1H-1H COSY, HMQC, HMBC, NOESY). RESULTS: Three steroidal saponins were isolated from the rhizomes of Dioscorea panthaica. They were elucidated as 26-O-beta-D-glucopyranosyl-3 beta, 26-diol-23(S)-methoxy-25(R)-delta 5,20(22)-diene-furosta-3-O-[alpha-L-rhamnopyranosyl-(1-->2)- O-alpha-L-rhamnopyranosyl-(1-->4)]-beta-D-glucopyranoside (I), pseudoprotodioscin (II), 26-O-beta-D-glucopyranosyl-3 beta, 26-diol-25 (R)-delta 5,20(22)-diene-furosta-3-O-alpha-L-rhamnopyranosyl- (1-->2)-O-beta-D-glucopyranoside (III). CONCLUSION: Compound I is a new steroidal saponin and was named as dioscoreside C. Compound II and III were obtained from this genus for the first time.

Dioscorea↗
PubMed2001

[Expression of BDNF and FGF-2 following axotomy in rat facial motoneurons].

OBJECTIVE: To study the expression and distribution of brain derived neurotrophic factor (BDNF) and fibroblast growth factor-2 (FGF-2) in normal facial motoneurons (FMNs) and in FMNs following axotomy. METHODS: The right facial nerves were transected 6 mm distal to the stylomastoid foraman in adult Wistar rats except the normal group. Serial 20 microns cryosections were cut through the whole brainstems. Expressions of BDNF and FGF-2 mRNA as well as BDNF and FGF-2 protein were studied by in situ hybridization, immunohistochemistry and image analysis. RESULTS: BDNF mRNA and its protein were observed in widespread areas of normal rat facial nucleus, and those increased 1 day after axotomy. In addition to neurons, glial cells were also stained. FGF-2 mRNA and its protein were mainly localized in normal FMNs of ventral facial nucleus. After axotomy, expression of FGF-2 mRNA started to up-regulate in FMNs at 7 days, however FGF-2 protein drastically reduced at 3 and 7 days. CONCLUSION: In addition to target-supporting, there may be BDNF autocrine and paracrine mechanisms as well as FGF-2 autocrine mechanisms in normal rat FMN. When target-derived BDNF is deprived, alternative sources of BDNF support may substitute immediately after axotomy. However, deprivating of target--derived FGF-2 may result in down-regulation of FGF-2 protein in adult rat FMNs at early stage after axotomy. These suggest that the exogenous FGF-2 might provide a supportive environment for the recovery of metabolism and function of FMNs at early stage following axotomy.

Animals↗
PubMed2000

Identification of two pairs of spatially approximated residues within the carboxyl terminus of secretin and its receptor.

The carboxyl-terminal domains of secretin family peptides have been shown to contain key determinants for high affinity binding to their receptors. In this work, we have examined the interaction between carboxyl-terminal residues within secretin and the prototypic secretin receptor. We previously utilized photoaffinity labeling to demonstrate spatial approximation between secretin residue 22 and the receptor domain that includes the first 30 residues of the amino terminus (Dong, M., Wang, Y., Pinon, D. I., Hadac, E. M., and Miller, L. J. (1999) J. Biol. Chem. 274, 903-909). Here, we further refined the site of labeling with the p-benzoyl-phenylalanine (Bpa(22)) probe to receptor residue Leu(17) using progressive cleavage of wild type and mutant secretin receptors (V13M and V16M) and sequence analysis. We also developed a new probe incorporating a photolabile Bpa at position 26 of secretin, closer to its carboxyl terminus. This analogue was also a potent agonist (EC(50) = 72 +/- 6 pm) and bound to the secretin receptor specifically and with high affinity (K(i) = 10.3 +/- 2.4 nm). It covalently labeled the secretin receptor at a single site saturably and specifically. This was localized to the segment between residues Gly(34) and Ala(41) using chemical and enzymatic cleavage of labeled wild type and A41M mutant receptor constructs and immunoprecipitation of epitope-tagged receptor fragments. Radiochemical sequencing identified the site of covalent attachment as residue Leu(36). These new insights, along with our recent report of contact between residue 6 within the amino-terminal half of secretin and this same amino-terminal region of this receptor (Dong, M., Wang, Y., Hadac, E. M., Pinon, D. I., Holicky, E. L., and Miller, L. J. (1999) J. Biol. Chem. 274, 19161-19167), support a key role for this region, making the molecular details of this interaction of major interest.

Alanine↗
PubMed2000

Comparison of K-ras point mutations at codon 12 and p21 expression in pancreatic cancer between Japanese and Chinese patients.

BACKGROUND AND OBJECTIVES: K-ras (Kirsten-ras) point mutation (PM) in codon 12 are suggested to be significantly associated with the tumorigenesis of pancreatic cancer. The incidences of K-ras PMs in human pancreatic cancer are reported to be different between Europeans and Japanese. The present study was designed to compare the incidences and profile of K-ras PMs and ras-p21 expression in primary invasive ductal carcinoma (IDC) of the pancreas between Japanese and Chinese. METHODS: The specimens included 51 Japanese and 34 Chinese patients with the primary IDC of the pancreas. K-ras PMs were tested by allele specific oligonucleotide dot blot hybridization methods and ras-p21 expression was stained by the immunohistochemical method. RESULTS: K-ras PMs were detected in 48 Japanese IDCs (94%) and in 24 Chinese ones (71%). There was a significant difference between the two groups. The GAT mutation was more frequent both in Japanese (61%, 33/54) and in Chinese (60%, 18/30) IDCs. The transitions/transversions ratio in the Japanese group was 2.4 in this study. By contrast, that in the Chinese group was 1.5. The expression of p21 was detected in 24 Japanese IDCs (47%) and in 24 Chinese IDCs (71%). There was a significant difference between the two groups. The expression of p21 and the patterns of K-ras PMs did not show any significant influence on the survival of the patients both in Japanese and Chinese. In the present study, Chinese IDC had a lower frequency of K-ras PMs in codon 12 than Japanese IDC. The pattern of K-ras PMs in Chinese IDC was different from that in Japanese and European IDC, respectively. CONCLUSIONS: Ki-ras PM and p21 expression were frequently seen both in Japanese and Chinese patients with pancreatic cancer. Factors such as lifestyle and environment may have influences on pancreatic carcinogenesis in various populations.

Aged↗
PubMed2000

Effects of pioglitazone on promoting energy storage, not expenditure, in brown adipose tissue of obese fa/fa Zucker rats: comparison to CL 316,243.

Recent advances in the treatment of non-insulin-dependent diabetes mellitus (NIDDM) include the use of thiazolidinediones (TZDs), agents that enhance insulin action, in part, through an activation of adipose tissue peroxisome proliferator-activated receptor gamma. Current evidence also indicates that these agents upregulate uncoupling protein 1 (UCP1) gene expression in brown adipocytes and increase interscapular brown adipose tissue (IBAT) mass in rodents, suggestive of a thermogenic component to their mechanism of action. In the present study, the TZD pioglitazone (PIO) and the beta3-adrenoceptor agonist CL 316,243 (CL), were used to determine whether the antidiabetic effects of PIO, like those of CL, may, in part, be mediated by an increase in either IBAT thermogenesis or whole-body energy expenditure. Treatment of obese, insulin resistant fa/fa Zucker rats with PIO for 10 days resulted in a 2- to 3-fold increase in IBAT mass, due largely to an increase in adipocyte size and number, and increased fatty acid biosynthesis. However, unlike the effects of CL, the PIO-induced IBAT changes were not associated with an increase in UCP1 expression or whole-body energy expenditure. In contrast to CL, PIO substantially increased body weight gains over the 10-day treatment period by increasing feeding efficiency. These data suggest that, unlike CL, the actions of PIO in the obese Zucker rat does not include increased energy expenditure, but rather strengthens its role as an adipogenic and lipogenic agent, which promotes energy storage.

Adipose Tissue, Brown↗
PubMed2000

Dual contacts between peptide agonist ligands and the secretin receptor directly established by photoaffinity labeling.

Structural analysis of secretin in solution has demonstrated extended helical domains within both amino- and carboxyl-terminal halves, with a possible turn in between. However, the conformation of this peptide as it resides in its binding site within the receptor has not been established. In the work reported here, we performed affinity labeling of the secretin receptor with radioiodinated secretin analogues having photolabile benzoyl-phenylalanine residues positioned in each half of the peptide. The probes had sites of covalent attachment in positions 6 and 22, and have been recently synthesized and characterized to represent high affinity agonist ligands. Both covalently labeled the secretin receptor in a saturable, specific, and efficient manner. After purification of the labeled receptor, we used a series of chemical and enzymatic cleavage techniques to define the domain of labeling. We complemented this by receptor mutagenesis, followed by additional cleavage and Edman degradation sequencing to refine our insights into the labeled residues. This has allowed us to demonstrate that sites of attachment were both within the extracellular aminoterminal domain of the receptor. Of particular interest, both probes labeled residues within the amino-terminal thirty residues at the distal end of the receptor. It will be particularly interesting to use these molecular approximations to model the binding domain of this important receptor.

Affinity Labels↗
PubMed2000

Structural insights into the amino-terminus of the secretin receptor: I. Status of cysteine and cystine residues.

The secretin receptor is prototypic of the class II family of G protein-coupled receptors, with a long extracellular amino-terminal domain containing six highly conserved Cys residues and one Cys residue (Cys(11)) that is present only in the most closely related family members. This domain is critical for function, with some component Cys residues believed to be involved in key disulfide bonds, although these have never been directly demonstrated. Here, we examine the functional importance of each of these residues and determine their involvement in disulfide bonds. Secretin binding was markedly diminished after treating cells with cell-impermeant reducing reagents, supporting the presence of important extracellular disulfide bonds. To determine whether the amino-terminal domain was covalently attached to the receptor body by disulfide linkage, a strategy was implemented that involved introduction of an acid-labile Asp-Pro sequence to enable specific cleavage at the boundary of these domains. Under nonreducing conditions, the amino terminus was released from the receptor body, supporting the absence of covalent association between these domains. Quantitative [(14)C]iodoacetamide incorporation into the isolated amino-terminal domain of the receptor in the absence and presence of chemical reduction established the ratio of free to total Cys residues as 1:7, consistent with three disulfide bonds. Mutagenesis of each of the amino-terminal Cys residues to Ala was tolerated only for Cys(11), suggesting that these bonds linked the conserved Cys residues. This was further supported by treatment of intact cells expressing wild-type or C11A mutant secretin receptor with a cell-impermeant sulfhydryl-reactive reagent. Thus, the functionally important amino terminus of the secretin receptor represents a structurally independent, highly folded, and disulfide-bonded domain, with a pattern that is likely critical and conserved throughout this receptor family.

Animals↗
PubMed2000

(10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid methyl ester as an anti-inflammatory compound from Ehretia dicksonii.

The methanol extract of Ehretia dicksonii provided (10E, 12Z, 15Z)-9-hydroxy-10,12,15-octadecatrienoic acid methyl ester (1) which was isolated as an anti-inflammatory compound. Compound 1 suppressed 12-Otetradecanoyl-phorbol-13-acetate (TPA)-induced inflammation on mouse ears at a dose of 500 microg (the inhibitory effect (IE) was 43%). Linolenic acid methyl ester did not inhibit this inflammation at the same dose. However, the related compounds of 1, (9Z,11E)-13hydroxy-9,11-octadecadienoic acid (5) and (9Z,llE)13-oxo-9,11-octadecadienoic acid (6), showed potent activity (IE500 microg of 63% and 79%, respectively). Compounds 1, 4 ((9Z, 12Z, 14E)-16-hydroxy-9,12,14-octadecatrienoic acid), 5 and 6 also showed inhibitory activity toward soybean lipoxygenase at a concentration of 10 microg/ml.

Animals↗
PubMed2000

Expression of transforming growth factor beta1 and its type II receptor in mouse colon tumors induced by azoxymethane.

Alterations in transforming growth factor beta1 (TGF-beta1) and its type II receptor (TbetaR-II) have been implicated in the pathogenesis of a variety of human cancers and animal tumor models. We postulated that TGF-beta1 and TbetaR-II alterations may also be involved in mouse colon tumorigenesis induced by the chemical carcinogen, azoxymethane (AOM). In the present study, normal colon tissues and AOM-induced colon tumors from SWR/J mice were analyzed for mutational changes in the TbetaR-II gene, and the expression and localization of TGF-beta1 and TbetaR-II were examined by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemisty. Direct DNA sequencing of the coding sequence of the TbetaR-II gene revealed no mutational changes in tumors induced by AOM when compared with the sequence from normal colon tissue. However, TGF-beta1 and TbetaR-II mRNA levels in tumor samples were increased 1.8-fold (p<0.01) and 1.3-fold (p<0.01), respectively, when compared with control mouse colon tissue. The results of immunohistochemical analysis of TGF-beta1 and TbetaR-II were correlated with mRNA expression data. An increase in staining intensity of both TGF-beta and TbetaR-II were observed in colon tumors. These findings suggest that alterations in the expression of TGF-beta1 and TbetaR-II may be involved in the pathogenesis of colon tumors induced by AOM in mice.

Adenocarcinoma↗
PubMed2000

Ki-ras point mutation and p53 expression in human pancreatic cancer: a comparative study among Chinese, Japanese, and Western patients.

The aim of this study was to clarify features of Ki-ras point mutation (PM) and p53 expression in Chinese pancreatic cancer and to compare those with that in other countries. Dot blot hybridization and immunohistochemical methods were performed in 59 Chinese patients. The results showed that Ki-ras PMs at codon 12 and p53 expression were frequent in this group. No relationships were found between Ki-ras PM alone and p53 expression alone, and clinicopathological parameters, including age, gender, clinical stage, and histological grade and classification in Chinese patients. However, their cooperation was significantly associated with a poor prognosis in this group. Comparison showed that there were significant differences in the overall frequency and substitution of Ki-ras PM and in the ratio of transition:transversion in pancreatic cancer among various countries. In addition, the effect of Ki-ras PM and p53 expression on a poor prognosis of pancreatic cancer may be different among various countries. These findings suggested that not only Ki-ras PM and p53 expression are frequent in Chinese pancreatic cancer, but also a gene component to pancreatic cancer may be different between Asian and Western pancreatic cancer. In addition, it seems that cooperation of Ki-ras PM and p53 expression may predict a poor prognosis in Chinese patients with pancreatic cancer.

Adult↗
PubMed2000

[Changes of maternal and umbilical serum nitric oxide in patients with the intrauterine growth retardation].

OBJECTIVE: To determine whether the levels of maternal and umbilical serum nitric oxide (NO) were changed in cases of intrauterine growth retardation (IUGR). METHODS: Fifteen pregnant women with fetuses of IUGR (IUGR group) and 25 normal pregnant women in late trimester (normal control group) were studied. Maternal and fetal umbilical venous blood were collected from all the cases. Serum NO2-/NO3-, the end products of NO, were measured with the Griess reaction after reduction with nitrate reductase. RESULTS: Mean NO2-/NO3- values in maternal serum were (74.22 +/- 28.99) mumol/L in IUGR group and (56.71 +/- 22.81) mumol/L in the control. Umbilical serum NO2-/NO3- values were (39.73 +/- 24.65) mumol/L in IUGR group and (21.49 +/- 6.69) mumol/L in the control. Compared with the control, maternal as well as umbilical serum NO2-/NO3- in IUGR group were significantly higher (P < 0.05 and P < 0.001, respectively). The total nitrite levels in fetal circulation were lower than in maternal circulation in both groups (P < 0.05). CONCLUSIONS: Changes of NO in the pregnant women and their fetuses were due to the result of secondary complementary response in the patients with IUGR. The metabolic disorder of nitric oxide in maternal and fetal circulation may play an important role in pathogenesis of IUGR.

Female↗
PubMed2000

Indirect neurotrophic effect of neuropeptide ZNC(C)PR on PC12 cells via peptide-stimulation of C6 cells.

AIM: To understand the mechanism of neurotrophic action of neuropeptide ZNC(C)PR and its effect on which could affect both growth and apoptosis of C6 cells. METHODS: Effects of ZNC(C)PR-treated C6 conditioned medium was observed on on culture of PC12 cells. The development of PC12 cells was determined by ratio of neurite-bearing cells in the total cells. The specific binding of ZNC(C)PR on C6 cells was determined by radioligand binding assay (RBA). RESULTS: ZNC(C)PR-treated C6 conditioned medium increased the ratio of neurite-bearing PC12 cells by 36% compared to the untreated C6 conditioned medium or to a mixture of ZNC(C)PR with the untreated C6 conditioned medium. RBA showed a specific binding site of ZNC(C)PR on C6 cells with Kd value of 2.74 nmol.L-1 and Bmax value of 19 pmol.g-1 protein. CONCLUSION: ZNC(C)PR enhanced C6 cells induced secretion of some neurotrophic factors which acted as enhancers for PC12 cells differentiation, through its specific receptor sites on the neuroglioma cell.

Animals↗
PubMed2000

[The effects of PDGF-AB on pro alpha 1 (III). mRNA expression of fibroblasts].

OBJECTIVE: To investigate the mechanism of PDGF-AB to promote wound healing and its role during the process of scar formation. METHODS: In vitro cultured fibroblasts of human normal skin and hypertrophic scar were treated with PDGF-AB. The expression of Pro alpha 1 (III) mRNA of the fibroblasts was evaluated by in situ hybridization technique. RESULTS: PDGF-AB could increase Pro alpha 1 (III) mRNA expression of fibroblasts of both normal skin and hypertrophic scar in a dose-dependent manner. However, the effect of PDGF-AB on hypertrophic scar fibroblasts was stronger. CONCLUSIONS: PDGF-AB may promote wound healing by stimulating extracellular matrix synthesis, e.g. collagen. PDGF-AB may also play a role in scar formation through collagen deposition.

Biomarkers↗