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Biomedical subjects

M Dolan

Publications and source records attributed to M Dolan.

At least 73 records · Page 4Linked to original sources

Co-morbidity: lessons learned about post-traumatic stress disorder (PTSD) from developing PTSD scales for the MMPI.

Results from efforts to develop and validate PTSD measures are promising, but a "gold standard" has not been achieved. Keane, Malloy, and Fairbank (1984) have developed an MMPI PTSD subscale that has been cross-validated with clinicians' classification of PTSD at acceptable levels of agreement, specificity, and sensitivity. There is, however, room for improvement. Empirical evidence is presented that indicates that the next round of efforts to increase reliability and validity of PTSD measures must account for the presence/absence of co-morbidity (i.e., the simultaneous occurrence of other psychiatric disorders). For example, differences are noted in MMPI group profiles and PTSD scales between psychiatric patients and substance abusers. Second, different MMPI items emerge as indicative of PTSD; these vary as a function of the presence of other Axis I disorders among groups of Vietnam combat veterans who seek treatment for substance abuse. Results substantiate that different MMPI items for classifying PTSD occur with groups that differ in co-morbidity. Improvements in PTSD scale development are more likely when the contributions of pre-existing or subsequently co-occurring psychiatric disorders are taken in account, as well as variations in level of personality maturity. The evidence suggests that a "family" of PTSD scales need to be developed that take into account co-morbidity differences.

Adaptation, Psychological↗

Ethnicity: post-traumatic stress disorder (PTSD) differences among black, white, and Hispanic veterans who differ in degrees of exposure to combat in Vietnam.

Clinical observations and empirical evidence suggest that, among Vietnam combat veterans, Blacks are more maladjusted than Whites (e.g., Parsons, 1985; Penk et al., 1985). The prediction that minority group status is associated with poorer post-war adjustment and higher rates of PTSD was examined among Vietnam combat veterans who were seeking treatment for addiction disorders. Adjustment scores among groups comparable in combat exposure were found to be similar for both Whites and Hispanics; Blacks, however, score significantly higher on both PTSD symptoms on MMPI scales. These findings indicate that ethnicity contributes importantly to PTSD in selected instances, but that minority group status alone does not account for observed differences. Additional research is indicated in which careful attention is given to the complicating and interacting role of addiction disorders in sampling.

Adaptation, Psychological↗

Transforming activity of DNA from rat liver tumors induced by the carcinogen methyl(acetoxymethyl)nitrosamine.

Altered c-Ha-ras genes have been frequently detected in the DNA of spontaneous or chemically induced mouse liver tumors. To determine if ras gene mutation is a frequent event during liver carcinogenesis in rats, we examined the transforming activity of DNA from liver tumors that developed in rats injected with methyl(acetoxymethyl)nitrosamine (DMN-OAc) after a partial hepatectomy. Three weeks after the injection of DMN-OAc, rats were fed a diet containing phenobarbital. This carcinogen acts only on replicating liver cells. Six of eight tumor DNAs induced the transformation of NIH 3T3 cells. The transforming activity was stable upon a second round of transfection, and the transformants were tumorigenic in nude mice. Southern blot analysis of transformant DNAs showed that the transforming activity was not due to the acquisition of a ras (Ha, Ki, or N), neu, myc, A-raf, v-raf, erbA, or erbB gene of rat origin. Several transformants' restriction enzyme sensitivity was analyzed, and their activity indicated that similar transforming sequences were present in at least two tumors and that one tumor contained two different transforming sequences. These results suggest that during hepatocarcinogenesis induced in rats by DMN-OAc, alterations in the ras gene family occur infrequently or not at all and that several different genes (which are not homologous to common oncogenes) become activated and are capable of transforming NIH 3T3 cells.

Animals↗

The gift.

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Aged↗

Hereditary copper toxicosis in West Highland white terriers.

Histologic, histochemical and atomic absorption studies on liver tissue from 71 West Highland white terriers are reported. Twenty-seven dogs had histologically normal liver and copper concentration comparable to mongrel control dogs. Forty-four dogs had hepatic copper concentrations up to 22 times the mean copper concentration found in clinically normal mongrel dogs. Hepatitis, hepatic necrosis and cirrhosis were associated with the increased copper concentration in some dogs. Matings between dogs with high liver copper concentration produced pups with high liver concentration. The copper storage defect is inherited.

Aging↗

Histochemical demonstration of copper and copper-associated protein in the canine liver.

Three different histochemical methods for copper detection were compared. Atomic absorption analysis was used to substantiate the tissue stains. There was good correlation between rhodanine staining and rubeanic acid-stained tissue sections. The orcein reaction for copper-associated protein did not consistently correlate with the methods demonstrating copper. Prolonged staining (72 hours) with rubeanic acid more consistently and clearly detected increased copper in canine livers than did staining with rhodanine. Seventy-two hour staining with rubeanic acid is the method of choice for histochemical detection of copper in canine liver.

Animals↗

Paraquat ingestion.

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Acute Kidney Injury↗

Serological and bacteriological survey of three horse studs for contagious equine metritis.

A bacteriological and serological survey for evidence of contagious equine metritis (CEM) was made during the 1980 breeding season on 3 horse studs in South Australia with a history of previous infection. Swabs from the clitoral sinus and the cervix were cultured for Haemophilus equigenitalis and serum was screened for antibody using the complement fixation test (CFT) and the enzyme-linked immunosorbent assay (ELISA). The specificity of both tests was greater than 0.99 but the ELISA was more sensitive in detecting antibody in infected mares. On the evidence presented it was concluded that H. equigenitalis is no longer present in the horse studs investigated.

Animals↗

The nucleotide sequence of the embryonic chicken beta-type globin genes.

The complete nucleotide sequence is reported for both of the embryonic chicken beta-type globin genes, rho and epsilon. These two genes lie at the 5' end and 3' end, respectively, of the four closely linked chicken beta-type globin genes relative to the direction of transcription. Both genes have structures that are typical of functional beta-type globin genes in that they contain two intervening sequences with the 5'-most intron being relatively small (108 base pairs in both) and the 3' intron being large (541 base pairs in rho and 973 base pairs in epsilon). Both embryonic genes contain consensus flanking sequences which are similar to those previously found in the adult chicken beta-globin gene and in a variety of other genes transcribed by RNA polymerase II. The rho- and epsilon-globin gene sequences are very similar from approximately nucleotide -130 (with the mRNA initiation site as +1) to the end of exon II. Noncoding sequences outside this region (e.g. intron II and DNA 5' to -130) are highly divergent. This may indicate that the DNA sequence elements which specifically activate embryonic beta-type globin gene expression in primitive red cells are contained within the -130 to +468 region. Comparison of the overall sequences of rho and epsilon to that of beta strongly supports the hypothesis that the embryonic avian beta-type globin genes diverged from the adult beta-gene by a process separate from that which was responsible for the generation of nonadult mammalian beta-type globin genes.

Amino Acid Sequence↗

Analysis of the adult chicken beta-globin gene. Nucleotide sequence of the locus, microheterogeneity at the 5'-end of beta-globin mRNA, and aberrant nuclear RNA species.

We have determined the nucleotide sequence of the adult chicken beta-globin gene locus. The structure of the adult chicken beta-globin gene is, for the most part, analogous to that of mammalian beta-globin genes thus far examined. The gene occupies 1525 nucleotides (nt) from the major cap site to the poly(A) addition site. Introns of 92 and 810 nt interrupt the coding sequence at positions corresponding to amino acid codons 30/31 and 104/105. The regions of most significant homology outside of the transcribed sequence occur within the 5'-untranslated region where the CCAAT and ATA boxes are found -74 and -30 nucleotides from the major chicken beta-globin mRNA CAP site. Using both primer-extension and mung bean nuclease mapping to localize the 5' terminus of the adult beta-globin mRNA within the transcription unit of this gene it was found that 5'-untranslated region is heterogeneous in length with termini 80 and 83 nucleotides from the translational initiation codon. In examining the transcription unit of this gene and the processing of its RNA species during maturation to mRNA, three distinct classes of beta-globin complementary nuclear RNAs are observed. One class of RNA consists of species whose size ranges from 1750 to 900 nt. These RNAs contain all or portions of the introns and are polyadenylated. Although the introns appear to be removed in a multistep fashion, a single kinetic processing pathway is not obvious. An additional processing pathway which leads to the accumulation of stable aberrant RNAs is also observed. This second class of RNA species contains first intron sequences, but lacks portions of the beta-globin coding sequence and thus is too small to become processed to functional mRNA. The third class of beta-globin complementary RNAs appear to migrate during gel electrophoresis at positions equivalent in size to RNAs of greater than gene length (2400-4500 nt), but are probably also never processed into mature mRNA.

Amino Acid Sequence↗

Intensive plasma exchange in small and critically ill pediatric patients: techniques and clinical outcome.

Standard apheresis techniques require modification of use in children, particularly those with serious concurrent medical problems, as they are prone to apheresis-induced disturbances of volume, metabolism, and coagulation. We report 112 plasma exchanges (TPE) on 11 children, 9 of whom weighed less than 20 kg and 7 of whom were critically ill. All were treated on continuous flow apparatus; seven on centrifugal systems (CS), two on a membrane filtration system (MFS), and two on both. Perturbations of blood and red blood cell (RBC) volume were prevented by priming the extracorporeal circuits with a red cell saline mixture having an hematocrit equal to or greater than the patient's hematocrit. Priming volume and minimal flow rates were 170 ml and 40 cc/min (MFS) and 350 ml and 10 cc/min (CS). TPE dose varied from 1.3 to 3 plasma volumes. Immunoglobulins fell by the following amounts: IgG 43.7%, IgA 36.7%, and IgM 41% per plasma volume. Platelets fell by 20-90% (CS) and 5-7% (MFS). Vascular access was obtained by various means including Thomas shunts, dialysis catheters, and standard 16-19 gauge butterflies and angiocaths. Bleeding in patients with coagulopathies was prevented by using repeated small boluses of heparin to maintain a clotting time of 2.5-3 minutes. Morbidity from TPE was limited to citrate toxicity (2 patients) and transient pulmonary edema (1 patient). Treatment outcome was successful in 8 out of 11 patients. We have shown that if PEX is otherwise indicated, it should not be withheld solely for patient size or the complexity of concurrent medical problems.

Adolescent↗

Chromosomal arrangement of the chicken beta-type globin genes.

We have isolated the chicken beta-type globin genes from a library of chicken DNA--lambda Charon 4A recombinant bacteriophage. There are four beta-type genes within this segment of the genome; we believe this represents all of the beta-type genes of the chicken. The recombinant lambda C beta G1 contains the embryonic epsilon- and adult beta-globin genes. The hatching beta H- and embryonic rho-globin genes are found in the recombinant lambda C beta G2. Although lambda C beta G1 and lambda C beta G2 do not physically overlap, we present evidence that all four genes are closely linked and transcribed from the same DNA strand. These experiments demonstrate that the chromosomal regions represented by lambda C beta G1 and lambda C beta G2 lie approximately 1.6 kb apart in the chicken genome. A third recombinant lambda C beta G3 extends the genomic locus studied in the vicinity of the beta-type globin genes to approximately 39 kb. The physical order of the chicken beta-type globin genes within this segment of the chromosome is 5' ... rho--beta H--beta--epsilon ... 3'. This arrangement is unique among the vertebrate beta-type globin gene clusters thus far examined, in that embryonic genes are located at the 5' and 3' ends of the cluster while the hatching and adult genes occupy central positions.

Animals↗