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M Dienst

Publications and source records attributed to M Dienst.

26 records · Page 2Linked to original sources

Postoperative follow-up and rehabilitation after meniscus replacement.

Even though basic scientific knowledge about the meniscal loading pattern may advocate restrictive rehabilitation after meniscus repair, experience and one controlled study favour accelerated rehabilitation. The current protocols for rehabilitation after meniscal substitution follow personal experience with parameters such as pain, effusion, locking, and gait pattern used as clinical guidance. Controlled clinical studies on rehabilitation should be encouraged. An example of a rehabilitation protocol is given. As meniscus replacement is a new treatment option, it is essential to document details about the graft, the knee status at operation and the surgical procedure. The goal of a postoperative follow-up is to control quality, to measure patient satisfaction and to show whether meniscus replacement is beneficial in relation to the natural history of meniscectomy cases. There is a need for a standard follow-up evaluation of patients after meniscus replacement, and the development of a meniscus transplantation score including subjective and objective data is suggested.

Humans↗

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Journal Article↗

Dynamic external fixation for distal radius fractures.

Thirty adult patients with closed comminuted and mostly intraarticular fractures of the distal radius were treated by closed reduction and immobilization with a dynamic external wrist fixator during a 2-year period. In 13 patients with severely comminuted and unstable fractures, additional Kirschner wires were used. After 10 to 14 days of rigid fixation in neutral position, the motion element was unlocked to allow up to 30 degrees flexion. Six weeks later, the fixator was removed. The patients then were observed for an average of 24 weeks. An excellent functional outcome was seen in 6 patients (20%), a good outcome in 20 patients (67%), and a fair outcome in 4 patients (13%). Anatomically, 15 patients (50%) had an excellent result, 14 (47%) a good outcome, and 1 (3%) a fair outcome. Only minimal loss of reduction averaging 1 degree palmar tilt was seen during mobilization. There were 2 major complications: 1 deep Kirschner wire tract infection and 1 index metacarpal fracture. Minor complications such as sensory disturbances and pin tenderness were present but recovered completely after removal of the fixator. This study provides promising data and offers an alternative method in the treatment of distal radius fractures with severe comminution. In cases with postreductive unstable fragments, additional Kirschner wires should be used to allow early mobilization of the wrist.

Adult↗

[Long-term outcome of 57 patients after ventral interbody spondylodesis of the lower cervical spine].

In this retrospective study we present the long-term results in 89 patients who underwent anterior interbody fusion of acute cervical spine injuries in our institution between 1972 and 1983. There were 23 dislocations, 50 fracture-dislocations and 16 burst fractures without significant posterior lesions. Only 20 patients showed no neurologic symptoms. In all 79 patients who were treated initially in our trauma department, closed reduction was performed as soon as possible. No aggravation of the neurologic conditions by this manoeuvre was observed. The mean time between injury and operation was 7 days (range 0-110 days). In 79 patients an H-plate or one-third-tubular plate was used, and in 10 patients fusion was performed only by bone grafting according to the technique of Robinson [40]. Severe complications, i.e. persistent instability with kyphosis or sagittal dislocation, were found in only 2 of these 10 patients secondary to technical problems. The radiologic results demonstrated a high fusion rate, with fusion in 78 out of the 79 patients. Of the 79 patients treated with plate spondylodeses, 7 devices had to be removed without loss of correction: 6 owing to loosening and 1 to breakage of the plate. Only in one additional case after early hardware removal because of loosening was a loss of correction detected. Of the original 89 patients, 17 had died of causes unrelated to the operation. The mean age of the surviving 72 patients at the time of injury was 36 years (14-83 years). A further 15 patients were lost during follow-up, so that 57 (79%) could be included in the study with a mean follow-up of 11 years, 9 months (10-19 years). The functional results were as follows: Active range of motion was limited between 25 and 50%. 40 patients had no pain at rest, 38 only during motion without need for treatment. A significant correlation between pain and fused segments could not be found. Spontaneous fusion and spondylophytes in adjacent motion segments were seen in 27 patients, again without significant correlation to residual pain. No hypermobility of adjacent motion segments in functional X-rays was seen. 46 patients returned to their former work, 7 were not able to work again secondary to the injury, 4 patients received old-age pension. Improvement of the neurologic deficits in the 37 symptomatic patients was observed in 30 cases (81.1%) according to the "Sunnybrook Cord Injury Scale" [49]; according to the ASIA Neurological Impairment Scale [5] 25 (67.6%) of them improved of at least 1 degree. No correlation could be found between the interval injury to operation and neurologic improvement. With regard to the early stage of the procedure of anterior interbody fusion for traumatic lesions this study shows promising data with high fusion rates and low morbidity, especially for the combination with plate fixation. Further improved within the last 12 years this method offers an excellent alternative for operative treatment of most acute lower cervical spine injuries. Using devices which are not angle stable, special attention should be given to precise bicortical screw fixation.

Adolescent↗

Growth inhibition in clonal subpopulations of a human epithelioid sarcoma cell line by retinoic acid and tumour necrosis factor alpha.

Epithelioid sarcoma is a highly malignant soft tissue tumour that is refractory to conventional chemotherapy and irradiation. Since permanent cell lines of this tumour are extremely rare, in vitro data on compounds with significant antiproliferative effects are still lacking. Therefore, we investigated the effects of retinoic acid (RA) and tumour necrosis factor alpha (TNF-alpha) on tumour cell proliferation of three different clonal subpopulations (GRU-1A, GRU-1B, GRU-1C) derived from the same human epithelioid sarcoma cell line, GRU-1. In GRU-1A both RA (P=0.01) and TNF-alpha (P=0.002) exhibited highly significant and dose-dependent growth inhibitory effects, which could further be increased by a combined application of both compounds (P<0.006). GRU-1B proved to be sensitive to RA (P=0.006), whereas no response to TNF-alpha was observed. GRU-1C was resistant to both RA and TNF-alpha. The antiproliferative effect of TNF-alpha was mediated by TNF receptor 1(TNF-R1) and correlated positively with both the number of TNF-R1 per cell and receptor affinity. No correlation was detected between RA-induced growth inhibition and the expression pattern of the RA receptors (RARs) RAR-alpha, RAR-beta, and RAR-gamma. Plating efficiency, however, could exclusively be reduced by RA in GRU-1B, the only cell line expressing RAR-alpha. Taken together, these data are the first showing significant antiproliferative effects in human epithelioid sarcoma by RA and TNF-alpha. Whereas the TNF-alpha response seems to depend on the expression of TNF-R1, no simple correlation could be found between RA sensitivity and the expression pattern of RARs.

Antineoplastic Agents↗

Characterization of a transglutaminase expressed in human pancreatic adenocarcinoma cells.

A soluble tissue type transglutaminase (TGases; R-glutaminylpeptide:amine gamma-glutamyltransferase, E.C.2.3.2.13) belonging to a group of widely distributed enzymes which catalyze the reaction between a gamma-carboxyamide group of a protein-bound glutamine residue and various amino groups was characterized in cell lines derived from human pancreatic carcinoma. The enzyme activity was measured by incorporation of [3H]putrescine into N,N-dimethylcasein. It showed a strong dependency on Ca2+, which could not be replaced by Mg2+ but was 80% inhibited by 0.7 mM Mg2+ in the presence of optimal Ca2+ concentration (7 mM). The Km-value in regard to putrescine was 2.6 mM. After centrifugation of cell homogenates at 105,000g 95% of the enzyme activity was found in the supernatant indicating that the TGase in pancreatic tumor cells is soluble. This was further substantiated by immunohistochemistry showing a homogeneous cytoplasmic distribution of the TGase in pancreatic tumor cells. Molecular sieve chromatography and Western blot analysis using an antibody against TGase II from human erythrocytes revealed a molecular mass of 80 kDa. In Northern blots with a cDNA of TGase II from mouse macrophages a single transcript approximately 3.4 kbp in size was detected. Polymerase chain reaction analysis using primers for the coding and 3'-non-coding regions showed in each case a single product with the size expected from the human cDNA of TGase II. Taken these data together, we conclude that human pancreatic adenocarcinoma cells express the soluble tissue type TGase II.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenocarcinoma↗

Hip arthroscopy without traction: In vivo anatomy of the peripheral hip joint cavity.

PURPOSE: To describe the in vivo anatomy of the peripheral compartment of the hip joint using a systematic sequence of examination without traction. TYPE OF STUDY: Case series. METHODS: We performed 35 hip arthroscopies without traction from an anterolateral portal in the supine position. Free draping and a good range of movement of the hip joint were used to relax parts of the capsule and increase the intra-articular volume of the area that was inspected. Each procedure was documented on a standard protocol including detailed information on technical features and normal and pathologic intra-articular findings. RESULTS: A comprehensive inspection of the peripheral compartment was obtained from the anterolateral portal. A systematic sequence of examination was developed separating the periphery of the hip joint into 7 areas: anterior neck area, medial neck area, medial head area, anterior head area, lateral head area, lateral neck area, and posterior area. The arthroscopic in vivo anatomy of each area is described. In 3 patients, 1 to 3 loose bodies were removed. In 1 patient with a synovial chondromatosis, 40 chondromas were retrieved. In osteoarthritis, impinging osteophytes were trimmed in 3 cases and partial synovectomy was performed in 10 patients. The following complications were observed: a temporary sensory deficit of the lateral femoral cutaneus nerve in 1 patient, scuffing of the anterior surface of the femoral head in 3 patients, detaching of an osteophyte in 1 patient, and partial tears of the anterior synovial fold in 10 patients. CONCLUSIONS: Arthroscopy without traction allows for a complete evaluation of hip anatomy without the loaded articular surfaces, the acetabular fossa, and the ligamentum teres. For a complete overview of both the central and peripheral part of the hip, traction is necessary for the central part.

Arthroscopy↗

Effects of TNF-alpha and retinoic acid on the proliferation of the clear cell and chromophilic types of human renal cell carcinoma in vitro.

Since no effective therapeutic approach is known so far for metastatic renal cell carcinoma (RCC), we analyzed the anti-proliferative effects of TNF-alpha and retinoic acid (RA), applied either alone or in combination on 7 different RCC cell lines in vitro. In 5 out of 7 cell lines, a significant (p < 0.05) dose-dependent inhibition of tumor cell proliferation became evident after exposure to TNF-alpha, the response being of modest magnitude in 5 cell lines. In 2 cell lines the effects were more pronounced with a reduction of cell viability to 55 +/- 11% of the control. Northern blot analysis revealed no expression of TNF-alpha and p75 TNF-receptor in any cell line. All the cell lines showed p55 TNF-receptor mRNA. Scatchard analysis revealed no correlation between TNF-alpha receptor status and growth inhibitory response to TNF-alpha, the number of TNF-receptors per cell ranging from 0 to 3,976, and the affinity values from Kd = 0.621 nmol/l to Kd = 4.28 nmol/l. Exposure to RA alone resulted in significant (p < 0.05), but modest growth inhibition in 2 out of 7 cell lines with a reduction of cell viability to 83 +/- 1% of the control. In 2 out of 7 cell lines, combination of RA and TNF-alpha was significantly (p < 0.05) more effective than the single application of each compound. Northern blot analysis revealed no transcripts of CRABP I and retinoic acid receptor (RAR)-beta. All the cell lines expressed RAR-alpha mRNA and one cell line additionally expressed RAR-gamma mRNA. A correlation between RAR status and RA response was not seen.

Adenocarcinoma, Clear Cell↗