The internal carotid artery and tumours of the neck.
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Adult↗
Biomedical subjects
M Desai
Publications and source records attributed to M Desai.
Correlation of cell surface fluorescence with conventional PAP analysis of cells of cytological interest obtained from cervical scrapes.
Archival PAP stained cervical smears were destained and treated with a fluorescent probe for a cell surface enzyme (GB). Cells which exhibited cell surface fluorescence were demonstrated to be cells of cytological interest in the analysis of cervical smears. These cells could be directly related to PAP and reclassified by subsequent restaining with PAP. Fluorescent cell surface technology was shown to be compatible with conventional PAP staining.
Cervix Uteri↗
Fluorescent location of cells of cytological interest in cervical smears prestained with thionin.
Cervical cells of cytological interest were located in conventional smears, treated with thionin for quantitative DNA staining by subsequent treatment with fluorescent probes for a cell surface protease. Normal mature cervical epithelial cells failed to bind these fluorescent probes whilst metaplastic cells, glandular cells, and dyskaryotic cells were readily located. By this means, the nuclear staining of these fluorescent cells of cyotological interest enabled them to be classified by a cytologist.
Carboxylic Ester Hydrolases↗
A simple clinical method for the preparation of improved cervical smears-approximating to monolayers.
Conventionally prepared cervical smears contain multilayers of cells deposited within strands of mucin. The present study is concerned with chemical reduction of disulphide bonds in the mucin leading to depolymerisation prior to forming a smear in the conventional manner. The resultant distribution of cells on the slide is similar to that obtained by machines designed to produce monolayers of cells. These monolayers have been developed for use in automated analysis of cervical smears and sputum samples. This new technique does not interfere with conventional PAP analysis of dyskaryotic cells nor does it interfere with the fluorescent location of such cells of cytological interest.
Dithiothreitol↗
Large myofascial fibromatosis involving the shoulder girdle.
An 11 year old girl presented with gradually increasing swelling over left scapular region and limitation of movements. On examination, a mass with firm to hard consistency was found fixed to the scapula. Clinical diagnosis of fibromatosis was confirmed by needle biopsy. Radical excision of the tumor was carried out and the resultant large exposed area was covered by transpositional rotation of trapezius myocutaneous flap. Microscopic examination of excised tumor revealed spindle shaped elongated cells in the form of whorls and trabeculae. The post operative course of the patient was uneventful with no evidence of recurrence at the end of 22 months.
Child↗