Search PubMed⌕ Search

Biomedical subjects

M Deminatti

Publications and source records attributed to M Deminatti.

At least 55 records · Page 3Linked to original sources
PubMed1976

[Compared cytologic study of (in situ and isolated) metaphasic chromosomes from KB cells, after incorporation of 5 bromodeoxyuridine (5 BrdU)].

Substitution of thymidine by 5 BrdU indduces the same morphological and cytological changes in in situ and isolated metaphase chromosomes of KB cells: a dichroïc fluorescence and a difference in the affinity of each chromatid for Giemsa stain. However, the precursor does not seem to modify the appearance of G or Q bands in in situ and isolated chromosomes. The fibrilar unit which can be seen with electronic microscopy, shows no difference before and after 5-BrdU incorporation.

Bromodeoxyuridine↗
PubMed1975

[Technique for isolation and purification of metaphase chromosomes of KB cells].

Metaphase chromosomes were isolated from KB cells according to Salzman and Mendelsohn's method. Whole-mount electronic microscopic preparations of chromosomes in situ, after isolation and after purification showed that the morphological patterns of this material are preserved in spite of physical and chemical treatments (pH 3).

Cell Fractionation↗