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Biomedical subjects

M Delaney

Publications and source records attributed to M Delaney.

50 records · Page 3Linked to original sources
PubMed1980

Radioimmunoassay of flurazepam in human plasma.

A radioimmunoassay for the determination of the hypnotic flurazepam in plasma was developed. Plasma levels of intact flurazepam were measured following oral administration of therapeutic doses to humans. The procedure employs an antiserum obtained from a rabbit immunized with 3-hemisuccinyloxyfluorazepam covalently coupled to bovine serum albumin and tritium-labeled flurazepam as the radioligand. Assay specificity was achieved by chromatographic isolation of flurazepam from plasma extracts on Sephadex LH-20 prior to analysis. The method has a sensitivity limit of 0.1 ng of flurazepam/ml using a 1-ml plasma sample, and the intra- and interassay coefficients of variation did not exceed 9% over a range of 0.1-5 ng/ml. In eight subjects who received a single 30-mg dose of flurazepam, peak plasma concentrations of 0.5-3.0 ng of intact drug/ml were reached after 0.5-1 hr, except in one subject where the peak occurred at 4 hr. The plasma concentrations of flurazepam declined with a harmonic mean apparent half-life of 2.3 hr.

Chromatography, Ion Exchange↗
PubMed1978

Affinity chromatography of bacterial lactate dehydrogenases.

The affinity system used was the immobilized oxamate derivative previously used to purify mammalian lactate dehydrogenases. The bacterial dehydrogenases specific for the L-stereoisomer of lactate behaved in the same way as the mammalian enzymes, binding strongly in the presence of NADH. The D-lactate-specific enzymes, however, did not show any biospecific affinity for this gel. The L-specific enzymes could be purified to homogeneity in one affinity-chromatographic step. The D-specific enzymes could be efficiently separated from the L-specific ones and could then be further purified on an immobilized NAD derivative. The mechanism of activation of the lactate dehydrogenase from Streptococcus faecalis by fructose 1,6-bisphosphate was investigated by using the immobilized oxamate gel.

Bacteria↗
PubMedDate not supplied

Economic and demographic consequences of AIDS in Namibia: rapid assessment of the costs.

Recent announcements by the Government of Namibia to provide financial support to people living with AIDS (and their family members) have received considerable media attention. However, given the fact that government budgets are already stretched, and the need for resources to devote the prevention efforts remains, there is an urgent need to assign some values to the support the government is considering within the context of an explosive epidemic. It is against this background that this study attempts to provide a rapid assessment of the economic costs of HIV/AIDS in Namibia over the next 5 years of the First National Development Plan. The estimates include the direct and indirect costs. The direct costs are costs to the economy for inpatient and outpatient medical services, as well as the costs of support payments to people living with AIDS, their families and children orphaned by AIDS. Government and donor expenditure on national prevention and control efforts are also included. The study concludes that no sector of the Namibian economy will escape the impact of AIDS. The epidemic will definitely tax hospital, public health, private and community resources, and these substantial burdens underscore the need for coordinated long-term planning.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Long-term care: workable options for employers now.

Lost in the labyrinth of long-term care benefits possibilities (or impossibilities)? There are ways that employers can begin today to provide at least a modicum of long-term care coverage for retired (and active) employees--and at very little cost. A long-term care consultant shares some practical approaches, along with insights into how federal government activities may affect employers' efforts.

Aged↗
PubMedDate not supplied

Signet-ring cell stromal tumor of ovary cytologic appearances of fine needle aspiration biopsy.

The cytologic appearances of fine needle aspiration biopsy from a benign signet-ring cell tumor of ovarian stroma are presented. The aspirate is characterized by the presence of many cells with large vacuoles in their cytoplasm giving them the signet-ring appearance. The lack of malignant criteria in the nuclei and the failure to demonstrate mucin or lipid by special stains helps to differentiate these cells from other sources of signet-ring cells such as mucinous cystadenomas and cystadenocarcinomas and Krukenberg tumors.

Biopsy, Needle↗
PubMedDate not supplied

Fine needle aspiration of ovarian masses. II. Correlative cytologic and histologic study of nonneoplastic cysts and noncelomic epithelial neoplasms.

A wide variety of neoplasms and nonneoplastic cysts involves the ovary. Seventy-seven ovarian masses were aspirated using fine needles. They included 32 nonneoplastic cysts of various types, 29 celomic epithelial neoplasms, 10 germ cell neoplasms and 6 other neoplasms. The study was performed in order to elucidate the cytologic features of aspirates from these lesions and correlate them with the histologic features. Material aspirated from malignant neoplasms resulted in abundantly cellular specimens while aspirates from benign neoplasms and nonneoplastic cysts showed only scanty cells. Follicular cysts and corpus luteum cysts showed similar cell types but with various degrees of cellularity and incidence of cell types. Cysts lined by a single layer of cuboidal cells, such as paraovarian and paratubal cysts, were very similar cytologically. The three types of germ cell neoplasms encountered showed features which were diagnostic of each entity. It was difficult to discriminate between some of the types of the sex cord and mesenchymal neoplasms. Aspiration cytology can achieve a satisfactory classification of ovarian masses. It has a specific role to play especially in young patients, in whom preservation of ovarian function is advisable.

Biopsy, Needle↗
PubMedDate not supplied

Fine needle aspiration of ovarian masses. I. Correlative cytologic and histologic study of celomic epithelial neoplasms.

The ovary is commonly involved by a wide variety of neoplasms and nonneoplastic masses. Seventy-seven ovarian masses were aspirated using fine needles. They included 29 neoplasms classified as being of celomic epithelial origin. The study was performed in order to elucidate the cytologic features of material aspirated from these neoplasms and to correlate them with the histologic features. As expected, material aspirated from malignant neoplasms was much more cellular than material obtained from benign neoplasms. By studying the background material, nuclear and cytoplasmic features of the epithelial cells, as well as the presence of other structures such as psammoma and hyaline bodies, a fairly specific pattern can be recognized for each neoplasm. Using aspiration cytology, a conclusion can be made regarding the malignant or benign nature as well as the specific type and classification of the ovarian neoplasms. Aspiration cytology, especially with the increased use of laparoscopy, has a specific role to play in the diagnosis of celomic epithelial neoplasms of the ovary. It is a valuable method when preservation of ovarian function or minimizing surgical trauma to the patient is desirable.

Adenocarcinoma↗
PubMedDate not supplied

The economics of a nurse-developed critical pathway.

During this study the use of a nurse-developed, patient-oriented critical pathway and its effects on two indicators of cost were analyzed at St. John Hospital and Medical Center in Detroit, Michigan. Financial data for 64 mastectomy cases were tracked and analyzed. Findings showed improved length of stay with substantial decreases in cost per case.

Cost-Benefit Analysis↗