Biomedical subjects
M Day
Publications and source records attributed to M Day.
Ethidium cooperativity in the formation of complexes with CpG.
The formation of complexes between the self-complementary ribo-dinucleoside monophosphate CpG and ethidium ion is observed by use of an ethidium ion selective electrode. The ratio of total CpG to total ethidium was varied from 50:1 to .4:1, with CpG concentrations ranging from 0.2 to 1.1 mM. Scatchard plots show that the system is strongly cooperative with respect to ethidium ion; cooperativity with respect to dinucleoside has been previously reported (Krugh, T.R., Wittlin, F.N., and Cramer, S.P. (1975) Biopolymers 14,197-210). Cooperative behavior with respect to ethidium ion implies the existence of complexes containing at least two molecules of ethidium ion in combination with one or more CpG molecules.
Computer-aided modeling in the assessment of the biomechanical determinants of diverse skeletal patterns.
Aberrant functional patterns of the masticatory musculature have been alleged as a factor influencing the morphogenesis of mandibular dysplasias. These include such expressions as the hyperdivergent skeletal open bite and its counterpart, the hypodivergent skeletal deep bite. A lack of anatomic data dealing with the actual musculoskeletal orientations of these types has resulted in speculative reports on divergent anatomy and its purported effects. In this study anatomic dissections of a hyperdivergent and a hypodivergent human cadaver illustrated that skeletally dysmorphic types possess different spatial orientations of the masticatory adductors in relation to dentofacial structures. A two-dimensional analysis of static equilibrium using computer-aided modeling demonstrated that these two types produce biomechanically distinct modes of muscle action. Temporalis activity must be 40% higher in the hyperdivergent model as compared with its counterpart to produce a biting force of 500 N (112 lb). The data suggest a rationale for the weak biting forces previously reported in long-faced persons when compared with horizontally developed subjects. The direction of condylar reaction force was also found to vary from a vertical orientation in the hyperdivergent model to oblique in the hypodivergent model. "Physis-like" behavior of the condyle, which can orient axially toward loading forces, may present an explanation for the reported differences in the direction of condylar growth in dysmorphic persons. A finite element analysis pictured distinct regions of deformation and compression in a novel perspective to help evaluate the currency of accepted hypotheses of stress-induced osteogenesis. The data support previous work reporting a latent geometric interdependency existing between muscle orientation and mandibular morphology.
Bacteriophage and their roles in biotechnology.
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Comparative effects of long-acting and conventional propranolol on stress-induced angina pectoris and on frequency of ventricular premature beats.
Ten male patients with chronic stable angina pectoris completed a randomized, double-blind cross-over study, with matched placebo run-in period (P), to compare the effects of a long-acting preparation of propranolol (LA, 160 mg once a day) with that of conventional propranolol (CP, 40 mg four times a day) each given for 14 days. Response was assessed by symptom-limited bicycle ergometry, degree of ST segment depression, daily anginal attack rate and glyceryl trinitrin consumption (GTN). Heart rate and ventricular extra-systolic frequency (VES) were recorded by 24 h Holter monitor. Bicycle ergometry was performed and a trough blood sample taken for propranolol estimation on day 14 prior to the morning dose. Both formulations increased total work capacity (P 3412, LA 4095, CP 3697 kpm/min), reduced rate-pressure product (P 21896, LA 16011, CP 15609 mm Hg beats/min), and degree of ST segment depression (P 4.53, LA 2.48, CP 2.43), but without differences between the formulations. Daily anginal attack rate was reduced from 30 (placebo) to 7.5 (CP) and 14.5 (LA) (P less than 0.05 between treatment groups). There was a reduction in daily GTN consumption by both treatments. The heart rate and total number of VESs during 24 h was similar in the two treatment groups and was reduced in comparison with placebo. Both formulations were well tolerated. Long-acting propranolol is an effective and well-tolerated alternative to conventional propranolol in the treatment of chronic stable and stress-induced angina, and in reducing VES frequency.
Immune complexes in systemic sclerosis; detection by C1q binding, K-cell inhibition and Raji cell radioimmunoassays.
Thirty-four patients with systemic sclerosis (SS) were investigated for the presence of circulating immune complexes by means of a fluid phase C1q binding assay, K-cell inhibition assay and a Raji cell radioimmunoassay and the results compared with those obtained in 21 patients with systemic lupus erythematosus (SLE) and 52 normal healthy controls. Patients with SS showed an incidence of circulating immune complexes comparable to that found in SLE, with 20 patients (58.5%), giving a positive result with at least one of the assays. The presence of circulating immune complexes in patients with SS was found to be associated with both elevation of serum IgG and IgA levels and extensive visceral involvement by the disease. These findings raise the possibility that circulating immune complexes could be involved in the pathogenesis of SS.
Short-term efficacy of oral verapamil in rest angina. A double-blind placebo controlled trial in CCU patients.
To determine the efficacy and safety of oral verapamil in patients with rest angina admitted to the Coronary Care Unit (CCU), a double-blind placebo-controlled trial was undertaken. Of the 65 patients with rest angina screened for the study, 15 met the inclusion criteria (at least two episodes of chest pain associated with ST-T segment changes per 24 hours) during single-blind placebo phase (Day 1). Patients were then randomized to receive either placebo or verapamil (80 mg every 6 hours) on Day 2. Protocol was designed such that those who did not respond to the placebo (nonresponders) received verapamil, 80 mg every 6 hours, whereas verapamil nonresponders received increased doses (120 mg every 6 hours) on Day 3. Those who did respond (responders) continued to receive their medication. Similar action was taken on Day 4, depending on chest pain frequency and clinical evaluation. The study drug was unblinded on Day 4. At the end of the four-day period, 13 patients were receiving verapamil (nine patients, 80 mg every 6 hours, and four patients, 120 mg every 6 hours) and all but one were responders. One patient received placebo all through the period of the study and was also considered to be a responder. In the remaining one patient evidence of myocardial necrosis developed after he received a single dose of verapamil (80 mg on Day 2). Except for the prolongation of PR interval in two patients while taking verapamil, no side effects from verapamil therapy were observed. These data demonstrate the efficacy of oral verapamil in reducing episodes of myocardial ischemia in the majority of all patients with rest angina.
Process in classical psychodynamic groups.
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Psychoanalytic group therapy in clinic and private practice.
The author reviews the process of psychoanalytic group psychotherapy in private practice and in the clinic setting. He describes the patients who are most likely to be referred for such therapy, most of whom have "failed" in other types of therapy. The group therapist begins with an empathic diagnostic evaluation of the patient's difficulty. As the patient engages in the group, he or she can compensate his or her ego; at the same time the patient develops a transference that is the main source of future work. In time, ego analysis and analysis of the infantile neurosis can be done. Finally, all the analytic work is consolidated during the process of termination.
Tracheobronchial cytologic changes and abnormal serum electrophoretic pattern during induced hypotension.
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Treatment of dysarthria: a case report.
There is little information available to the speech clinician on management of dysarthria. This article presents the rationale, procedural outline, and results of an approach to communication training for one dysarthic subject in view of suggestions made by Darley et al. (1975).
Rapid concentration of bacteriophages from aquatic habitats.
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Genetic analysis of amidase mutants of Pseudomonas aeruginosa.
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Hemogram of the Arabian horse.
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Evaluation of 'Alcotest R80' reagent tubes.
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Precision of urine: whole blood and urine: plasma ratio for alcohol.
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