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Biomedical subjects

M Daniel

Publications and source records attributed to M Daniel.

At least 217 records · Page 12Linked to original sources
PubMedDate not supplied

CT in adrenal enlargement due to tuberculosis: a review of literature with five new cases.

Tubercular adrenalitis presents with adrenal enlargement prior to its atrophy and calcification. Imaging modalities like ultrasound, computerized tomography (CT), and magnetic resonance imaging have depicted adrenal enlargement due to various causes. Presented is a clinical-cum-CT review of 14 cases of tubercular adrenal enlargement with addition of five new cases and their CT follow-up.

Addison Disease↗
PubMedDate not supplied

Complex chromosome abnormality mimicking t(8;21)in an acute myeloblastic leukemia.

A patient with acute myeloblastic leukemia (AML) and karyotypic abnormality mimicking t(8;21) is reported. Because of the uncommon morphology of blasts for AML with t(8;21), and absence of AML1-ETO fusion in the RT-PCR analysis, fluorescence in situ hybridization (FISH) was applied to precise the abnormality. FISH revealed a complex rearrangement involving chromosomes 7, 21 and 8, and involvement of the AML1 gene without evidence of AML1-ETO fusion. This case of unusual AML illustrates the necessity of collecting all hematologic, cytogenetic, and eventual FISH and RT-PCR data to analyse genetic rearrangements in leukemic patients.

Adult↗
PubMedDate not supplied

Bone mineral density and adipose tissue distribution in young women: relationship to smoking status.

Interrelationships between anthropometric variables and bone mineral density (BMD) may allow simple and inexpensive identification of those at risk for low bone density and osteoporosis. Risk appraisal is particularly important in young women, as lifestyle modifications may slow the rate of bone loss through adulthood and decrease the risk of osteoporosis later in life. Though weight, height and body mass index are frequently assessed in relation to bone, adipose tissue distribution has rarely been considered. Smoking is associated with low bone density and abdominally localized adipose tissue. The relationship between smoking, adipose tissue distribution and BMD in women has not been examined. Regional and whole-body BMD were assessed by dual-energy X-ray absorptiometry, and adipose tissue distribution using the anthropometric indicator, waist-to-hip girth ratio (WHR), in 52 women (25 smokers, and 27 nonsmokers) aged 20-35 years. There were significant (p < 0.05) positive correlations between WHR and BMD for smokers and nonsmokers separately (r = 0.44-0.57), and for the sample overall (r = 0.30-0.51). Most relationships remained significant after adjustment for weight. Smokers did not differ significantly from nonsmokers in relationships between WHR and BMD. The results indicate a positive relationship between BMD and abdominal relative to gluteal-femoral adiposity. This relationship is independent of smoking status. The biological determinants underlying a relationship between BMD and WHR require consideration before inferences regarding BMD can reasonably be considered on the basis of WHR.

Adipose Tissue↗
PubMedDate not supplied

Lectin-binding sites on human sperm during acrosome reaction: modifications judged by electron microscopy/flow cytometry.

Biochemical surface modifications occur during the capacitation and acrosome reaction of human sperm and among those, variations in the expression of carbohydrates moieties. A sequential study was performed with electronic microscopy and flow cytometry techniques, where the binding of 4 lectins was assessed on normal human sperm samples during in vitro induction of the acrosome reaction with calcium ionophore A-23187. Triticum vulgaris agglutinin (WGA) was shown to bind strongly the whole surface of sperm before induction of the acrosome reaction, and in lesser amounts after incubation with calcium ionophore. Arachis hypogea agglutinin (PNA) and mostly Concanavalia ensiformis agglutinin (Con-A) and Ulex europaeus agglutinin (UEA-1) binding evolved in an opposite pattern with an increase of the labeling parallel to that of GB24 antibody binding. Electron microscopy showed that the fluorescence patterns observed correlated with increased access to the inner membrane of the acrosome. This was significant 60 min after the induction of acrosome reaction. Lectin binding could be a useful tool to examine the ability of sperm samples to undergo the acrosome reaction.

Acrosome↗
PubMedDate not supplied

[Effect of microclimate on the development of parasitic arthropods].

The paper contains a survey of methods used and experience gained during the 25-year research of the influence of microclimate on the existence and development of parasitic arthropods at the Institute of Parasitology, Czechoslovak Academy of Sciences. Research methods by means of apparatuses, organization of long-term experiments and procedures in the assessment of results are described. An emphasis is put on the inter-disciplinary character of such working trend and on the advantage of creating complex working teams, in which the biologist must be the initiator and coordinator of the studies.

Academies and Institutes↗
PubMedDate not supplied

[Biochemical characterization and study by quantitative autoradiography of the binding sites of indalpine, a 5-HT uptake inhibitor, in cat brain].

The [3H]indalpine binding sites have been characterized in slide-mounted cat brain sections. This inhibitor of 5-HT reuptake binds with a very high affinity to sites which have the pharmacological properties of the serotonin carrier. These sites can, however, be differentiated from the [3H]imipramine binding sites by their Na+ dependency and competitive inhibition by serotonin. Quantitative autoradiographic studies demonstrate that indalpine binding sites are localized in structures rich in serotonergic neurons. The widespread distribution of indalpine binding sites in limbic and associative areas is consisted with its well characterized antidepressant activity in human.

Animals↗