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Biomedical subjects

M Dabrowska

Publications and source records attributed to M Dabrowska.

At least 37 records · Page 2Linked to original sources
PubMed2000

Molecular modelling, synthesis and antitumour activity of carbocyclic analogues of netropsin and distamycin--new carriers of alkylating elements.

A series of netropsin and distamycin analogues was synthesised and investigated by molecular modelling. The lowest-energy conformations of four carbocyclic lexitropsins, potential carriers of alkylating elements, were obtained using the HyperChem 4.0 program, and compared with the DNA-lexitropsin crystal structures from the Brookhaven National Laboratory Protein Data Bank. A method for synthesis of carbocyclic lexitropsins was elaborated, with the use of a nitro group or azobenzene as precursors for the aromatic amino group. The influence of methoxy group in ortho position with respect to amide groups on the activity of the new compounds was investigated. All of the compounds tested showed high antitumour activity in the standard cell line of mammalian tumour MCF-7.

Alkylating Agents↗
PubMed2000

[Septic-toxic shock due to rhabdomyolysis in a patient treated with fenofibrate].

Lipid disorders have been treated with fibrates for many years. Rhabdomyolysis is one of the side effects of these drugs. We report a case of a septic-toxic shock due to rhabdomyolysis in a 75-year old patient, who had been treated with fenofibrate for 2 years. This case shows necessity of the standard monitoring of aminotransferase, phosphocreatine kinase and creatinine levels during treatment with fibrates.

Aged↗
PubMed2000

[Biochemical mechanism of raloxifen and tamoxifen action for the prevention of breast cancer. Studies in vitro].

Cancer growth follows fine balance disturbance between cell proliferation, differentiation and death. It has been shown that mutation of 4 to 5 genes controlling cellular proliferation events may be contributive to carcinogenesis. Estrogens play a central role in reproductive physiology. They are also a causative factors in the pathogenesis of neoplastic and non-neoplastic diseases, including breast cancer. The estrogen dependency of human breast cancer has been successfully exploited in the treatment of early and advanced diseases and provides a unique opportunity for chemoprevention of this common malignancy. The aim of present study was to examine the effects of tamoxifen and raloxifen on the induction of apoptosis and proliferative activity of human breast adenocarcinoma MCF-7 cells. It has been found that both tamoxifen and raloxifen decreased the speed of cell cycle in MCF-7 cells and acts as proapoptotic factors. It reduces viability of cancer cells and probability of neoplastic clone multiplification. This effect conducts to limitation of cancer expansion.

Adenocarcinoma↗
PubMed2000

[Levels of alpha-2-macroglobulin in blood serum of women giving birth to hypotrophic and eutrophic newborns].

We tested the concentration of alpha-2-macroglobulin in sera of 33 women bearing eutrophic newborns, 36 women bearing hypotrophic newborns and 30 healthy not pregnant women in reproductive age. The concentration of this inhibitor was measured using radial immunodiffusion method according to Mancini et al. We found distinct decrease of alpha-2-macroglobulin concentration in sera of bearing women. In women bearing eutrophic newborns we found 154 mg/dl, in women bearing hypo-trophic newborns 171 mg/dl whereas in controls 250 mg/dl. We have noted statistically significant differences between tested groups to controls and between investigated groups. Taking under consideration the role of alpha-2-macroglobulin as the modulator of immune system as well as the activity of several cytokins, therefore one can suppose that alpha-2-M may affect on cellular growth developed foetus in intrauterine.

Adult↗
PubMed2000

An assessment of lymphocytic population in peripheral blood of patients with renal cell carcinoma before and after embolization.

The immunophenotype of peripheral blood lymphocytes was evaluated in 50 renal cell carcinoma (RCC) patients, before and after embolization. In RCC patients we have observed significant decrease in the total lymphocyte number as well as in T lymphocyte, their subpopulations and B lymphocyte number. Ten to twelve months after the treatment by embolization, significant increase in the total number of not only lymphocytes (p < 0.001), including T lymphocytes (p < 0.001), T-helper lymphocytes (p < 0.05), cytotoxic T lymphocytes (p < 0.05) and B lymphocytes (p < 0.05), but also an increase of NK cells number (p < 0.05) were found, compared to the values obtained before the treatment and reached the level observed in healthy subjects. Cytometric evaluation of peripheral blood lymphocytic phenotype in renal cell carcinoma patients can help evaluate the immune status of RCC patients as well as assess the embolization efficiency.

Adult↗
PubMed1999

[The concentration of alpha-2-antiplasmin in sera of women in post partum].

Alpha-2-antiplasmin is a main inhibitor of plasmin and play a crucial role in regulation of intravascular fibrinolysis. It is important to know, in pregnant women as well in the immediate post partum period the activation of hemostasis takes place and is observed thrombotic incidences. In our studies we have included 33 pregnant women whose delivered eutrophic newborns alpha-2-AP 7.79 mg/dl and 36 pregnant women whose delivered small-for-date newborns, alpha-2-AP 7.49 mg/dl. We found no significant statistically differences between tested groups and in comparison to control group 6.0 mg/dl. The presented results seems to indicate, the determination of concentration of alpha-2-AP in sera of women in immediate post partum period does not reflect the changes in the mechanisms of fibrinolytic system in the course of the partum.

Female↗
PubMed1999

[Flow cytometry in hematologic diagnosis].

The paper presents flow cytometry method in haematology diagnostics. Beside to the estimation of blast cells phenotype in acute leukaemia, flow cytometry allows to evaluate the presence of minimal residual disease (MRD) and multi drug resistance protein (MDR). Flow cytometry plays a crucial role in the estimation of DNA profile in cancer cells and evaluation of stem cells. Repeatedly flow cytometry is used to estimate platelets and reticulocytes.

DNA, Neoplasm↗
PubMed1999

The concentration of alpha-1-antichymotrypsin in blood serum of women in labour.

Concentration of alpha-1-antichymotrypsin (A-1-ACT) in blood serum of parturient women was determined. The investigation was conducted in 33 women bearing eutrophic newborns and 36 women bearing hypotrophic newborns. The control group consisted of 30 healthy non-pregnant women in reproductive age. Concentrations of A-1-ACT were determined using the radial immunodiffusion method according to Mancini et al. Maximal concentration of A-1-ACT determined in group I was three times higher than minimal concentration. Maximal concentration of A-1-ACT determined in group II was two times higher than minimal concentration. In the control group, the difference between minimal and maximal concentrations of A-1-ACT was inconsiderable. The lack of statistically significant differences between these three groups suggests that labour stress does not influence serum concentrations of this inhibitor. The importance of A-1-ACT in the placental tissue may be connected with immunological mechanisms that assure development and maintenance of pregnancy.

Female↗
PubMed1998

Comparison of oxidative base damage in mitochondrial and nuclear DNA.

The levels of endogenous pig liver cells mitochondrial DNA oxidative base damage have been investigated using isotope dilution gas chromatography mass spectrometry (GC/MS). Higher levels of five measured bases were found in mtDNA in relation to nuclear DNA. We have also detected large differences in the modified base ratios of mitochondrial versus nuclear DNA. These ratios for the bases with promutagenic properties as 8OHGua and 5OHCyt are much lower than for other bases (5OHHyd, 5OHMeHyd, 5OHMeUra).

Animals↗
PubMed1998

[Antiproteases in the pathogenesis of neoplastic diseases].

The imbalance between proteases and their inhibitors has substantial significance for the pathogenesis of the neoplastic disease. Proteases have been shown to play multiple roles in carcinogenesis and tumor promotion, while antiproteases (PI) can be regarded as anticarcinogenic agents. Hypotheses concerning the mechanism of PI anticarcinogenic action were presented in this article. The estimation of the antiprotease level may be clinically and therapeutically useful in some cases of cancer disease.

Antimetabolites, Antineoplastic↗
PubMed1998

[Flow cytometric analysis of cord blood lymphocytes].

It has been observed that immunological reactivity of neonatal leukocytes is diminished. It seems to result from lymphocytes immaturity. In the present study we have evaluated the phenotype of cord blood lymphocytes with the use of flow cytometry. In neonates we have observed the increase in the absolute number of CD1a and CD7 positive lymphocytes as well as CD2, CD3, CD4, CD8, CD16, CD19 and CD20 positive, while number of CD57 positive cells was significantly decreased. The ratios of CD4/CD8 and T/B lymphocytes were similar to the corresponding ratios observed in adults. Our results have shown the presence of immature lymphocyte population as well quantitative depletion of subpopulation of CD57 cells, what may pose an increased risk of infection in neonates. Nevertheless, increased number of T and B lymphocytes with mature phenotype along with normal ratio of lymphocyte subpopulations and increased number of CD16 positive cells (NK cells), may explain phenomenon of good health among majority of newborns.

Adult↗
PubMed1998

[Serine protease inhibitors in newborns. IV. Levels of alpha-2-macroglobulin in serum of eutrophic and hypotrophic newborns and their mothers].

It is accepted that alpha-2-M may serve as an important factor for the regulation of homeostasis. For these reasons it is valuable to determine its concentration in some clinical conditions. We have found that the level of alpha-2-M is evidently higher in hypotrophic newborns than in neutrophic newborns. It is interesting to note that the mothers of hypotrophic newborns also exhibit increased concentration of alpha-2-M in comparison to the mothers of eutrophic newborns. No differences were observed in concentration of this inhibitor between mothers and this newborns in both groups. Taking under consideration these findings it seems there is an inverse relationship between cord serum alpha-2-M concentrations and birth weight of newborns. Probably the increase of this inhibitor may reflect in some extent its function as moderating factor high susceptibility hypotrophic newborns for infections since alpha-2-M plays so crucial role in inflammatory processes.

Adult↗
PubMed1998

Middle superficial cerebral vein.

The microanatomical study present the anatomy of the middle cerebral vein. On the basis of the anatomical data we distinguished concentric and nonconcentric types of tributaries of the middle superficial cerebral vein. The nonconcentric type prevails.

Cerebral Veins↗
PubMed1997

Does the concentration of alpha 1-proteinase inhibitor reflect the transformation of liver cirrhosis to liver carcinoma?

Serum concentration of alpha1-proteinase inhibitor (alpha1-PI) was determined by nephelometric method in forty seven patients with liver cirrhosis (LC) and fifteen patients with hepatocellular carcinoma (HCC). Our data show an increase in the concentration of alpha1-PI in the group with decompensated LC by 17% and in HCC by 29%. The level of alpha1-PI higher than (220 mg%) may be indicative of the disease progression towards decompensated LC or HCC. In the conclusion, an increase in alpha1-PI concentration in patients with LC may be considered as an alarming factor, but is not sufficiently specific to become a diagnostic tool for the detection of HCC development.

Adult↗
PubMed1996

A general system for generating unlabelled gene replacements in bacterial chromosomes.

A general system is described that facilitates gene replacements such that the recombinant strains are not labelled with antibiotic resistance genes. The method is based on the conditional replication of derivatives of the lactococcal plasmid pWV01, which lacks the repA gene encoding the replication initiation protein. Replacement vectors can be constructed in and isolated from gram-positive and gram-negative helper strains that provide RepA in trans. Cointegrate formation of the integration vectors with the chromosome of the target strain is selected by antibiotic resistance. Resolution of the cointegrate structure is identified in the second step of the procedure by the loss of the lacZ reporter gene present in the delivery vector. The second recombination event results either in gene replacement or in restoration of the original copy of the gene. As no antibiotic resistance marker is present in the genome of the mutant the system can be used to introduce multiple mutations in one strain. A feasibility study was performed using Lactococcus lactis and Bacillus subtilis as model organisms. The results indicate that the method should be applicable to any non-essential gene in numerous bacterial species.

Bacillus subtilis↗