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Biomedical subjects

M D Turner

Publications and source records attributed to M D Turner.

At least 91 records · Page 5Linked to original sources
PubMed1970

Studies on the heterogeneity of human gastric zymogens.

THE PEPTIC ZYMOGENS OF HUMAN GASTRIC MUCOSA HAVE BEEN STUDIED IN THE PAST BY THREE DIFFERENT TECHNIQUES: phosphate-gradient chromatography on a column of DEAE-cellulose, chloride-gradient chromatography on DEAE-cellulose and agar-gel electrophoresis. These techniques have given different results, each suggesting the presence of a different number of pepsin zymogens in human gastric mucosa. In the present experiments gastric mucosal homogenates were subjected to analysis by all three techniques. The two major zymogen peaks obtained from phosphate-gradient chromatography were found to be composed of the three fractions found on chloride-gradient chromatography; these fractions in turn were shown to be heterogeneous on agar-gel electrophoresis. The latter technique demonstrated the presence of seven separable enzymically active components in human gastric mucosal homogenates. The relationships of the components separated by the three techniques are discussed.

Agar↗
PubMed1970

Pepsin secretion in man after Histalog stimulation.

Human gastric juice shows three separable pepsin fractions on chromatography on DEAE-cellulose. The response of these fractions to stimulation with beta-aminoethylpyrazole hydrochloride (Histalog) was studied. After a single intramuscular injection of Histalog (1.7 mg/kg body weight) there was an increase in the output of all three chromatographically distinguishable pepsin fractions. Little change was observed in the relative proportions of these three fractions and there was no evidence of selective stimulation of any individual fraction.

Chromatography, DEAE-Cellulose↗