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Biomedical subjects

M D Hoffman

Publications and source records attributed to M D Hoffman.

At least 37 records · Page 2Linked to original sources
PubMed1996

Relationships among heart rate, lactate concentration, and perceived effort for different types of rhythmic exercise in women.

OBJECTIVE: Exercise training intensity for aerobic conditioning is typically established by heart rate (HR), oxygen uptake, or rating of perceived exertion (RPE). Recent research, however, suggests that the optimal training intensity may be more appropriately established from measurements of blood lactate concentration ([La]). This study examined the relationships among three of these training intensity variables--HR, RPE, and [La]--for six modes of rhythmic exercise. DESIGN: Ten healthy women subjects underwent a 4-week habituation period to become familiar with the RPE scale and exercise on a treadmill, cycle ergometer, rowing ergometer, Airdyne, stairstepper, and cross-country skiing simulator. Following habituation, each subject underwent graded discontinuation exercise testing on each mode. HR was measured during the last minute of each 4-minute stage. Immediately after each stage, RPE was requested and blood was collected for analysis of [La]. Data were analyzed with repeated measures ANOVA. RESULTS: For given RPE values, the treadmill induced higher (p < .05) HR values compared with the cycle and rowing ergometers, and the cycle ergometer induced lower (p < .05) HR values compared with the treadmill, Airdyne, stairstepper, and cross-country skiing simulator. The relationships of [La] with RPE were similar among modes except for the cross-country skiing simulator, which induced a lower (p < .05) [La] for a given RPE. CONCLUSIONS: Since the relationships of HR and [La] with RPE are not the same for all forms of rhythmic exercise that use a large muscle mass, we conclude that mode specificity should be considered when prescribing aerobic exercise.

Adult↗
PubMed1996

Acute effects of acupuncture on physiological and psychological responses to cycle ergometry.

OBJECTIVE: Recent research has reported an improvement in exercise capacity following a series of acupuncture treatments. We examined the immediate effects of a single acupuncture treatment on perceived exertion and physiological responses during submaximal dynamic exercise, and maximal exercise capacity in human subjects. DESIGN: Ten healthy subjects (5 men, 5 women) participated in the study. After demonstrating consistency in using the rating of perceived exertion (RPE) scale, each subject underwent graded continuous maximal exercise testing on a cycle ergometer after genuine acupuncture, sham acupuncture, and rest without needles being inserted. Treatment conditions were randomized for each subject, and tests were conducted at least 72 hours apart. Heart rate and oxygen uptake were measured, and RPE was requested during the last 30 seconds of each 3-minute exercise stage. Data were analyzed with repeated-measures analysis of variance. RESULTS: There were no statistically significant differences among conditions for RPE, oxygen uptake, heart rate, respiratory exchange ratio, ventilation, and ventilatory equivalent for oxygen at submaximal workloads. In addition, none of the physiological measures at peak exertion or the duration of exercise differed among conditions. CONCLUSIONS: A single acupuncture treatment has no significant immediate effect on the perceived exertion and physiological responses during submaximal dynamic exercise or upon maximal exercise capacity among healthy individuals.

Acupuncture Therapy↗
PubMed1996

Linkage studies of NIDDM with 23 chromosome 11 markers in a sample of whites of northern European descent.

Considerable data support a genetic basis to susceptibility for NIDDM, but previous analysis of candidate genes has failed to identify a major susceptibility locus. Among regions with multiple potential candidates is chromosome 11, which includes the apolipoprotein C3 cluster, muscle glycogen phosphorylase, two insulin-dependent diabetes loci, the sulfonylurea receptor, and ataxia telangiectasia. To test linkage, we initially typed 19 markers at 10- to 15-cM intervals along chromosome 11. Analyses carried out under parametric models in members of 16-19 families of northern European ancestry detected possible linkage of NIDDM to D11S916. Nonparametric methods detected possible linkage to NIDDM at D11S901, which was 5- 10 cM distant, and at D11S935, which was approximately 30 cM distant. Both D11S916 and D11S901 were near the IDDM4 locus. To further test linkage, we typed five additional markers within 5 cM of D11S916 in the initial 19 families. We also tested markers from the linked region in a second set of recently sampled additional families. Two additional markers (D11S527 and D11S534) showed possible linkage in the initial 19 families, but none of the markers were linked to NIDDM in a separate set of families from the same ethnic background. The best evidence for linkage in the combined data set of the initial 19 families and 26 additional families was at D11S534 under parametric analysis (Z = 1.20) and at D11S935 under nonparametric analysis (affected pedigree number, P = 0.0013). Our findings suggest marginal evidence for a diabetes susceptibility locus in the region between D11S901 and D11S935, with the best evidence for a locus at or near D11S935. Replication of these findings in other populations will be necessary to distinguish false-positive linkage from a true NIDDM susceptibility locus.

Adult↗
PubMed1996

Role of mitochondrial DNA tRNA leucine and glucagon receptor missense mutations in Utah white diabetic patients.

Recently, subtypes of typical NIDDM were suggested based on missense mutations of mitochondrial DNA [tRNALeu(UUR)] and the glucagon receptor gene (Gly40Ser). Together these mutations might explain NIDDM in 5--8% of patients. To test the hypothesis that these mutations play an important role in a Northern European population with a strong family history of diabetes, we screened members of 45 families selected for having two or more diabetic siblings and 62 additional unrelated diabetic individuals for both mutations. We also examined 74 nondiabetic control subjects. Mitochondrial DNA mutations were not detected despite our ability to detect as little as 3% heteroplasmy in a sample from an individual known to carry the mutation. Likewise, the glucagon receptor Gly40Ser mutation was present in a single diabetic patient who on subsequent investigation was of Italian descent. Thus, neither subtype of NIDDM is present in the Utah diabetic population, which is reflective of other Northern European populations.

Alleles↗
PubMed1995

Delta efficiency of uphill roller skiing with the double pole and diagonal stride techniques.

Delta efficiencies for uphill roller skiing with the double pole (DP) and diagonal stride (DS) techniques were determined among 4 female and 4 male cross-country ski racers in order to examine for differences between techniques and between the sexes. Each skier roller-skied on a motorized ski-treadmill at 1.7% and 7.1% grades with both techniques at 2 to 4 different speeds. Steady-state oxygen uptake values were used to calculate the differences in metabolic requirements for roller skiing at the 2 grades (delta E). The differences in external work rates between the 2 grades (delta W) were calculated from the work rates for overcoming rolling resistance and elevating the transported mass against gravity. Delta efficiencies (delta W/ delta E) ranged from 14 to 36%, were significantly greater (p < 0.001) for DS than DP, and showed a significant (p < 0.01) velocity effect for DS. Delta efficiencies were 27% greater (p < 0.05) for the women compared with the men for DP, and significant (p < 0.05) correlations were found between efficiency for DP and body mass. This suggests that the higher efficiency with DP for female skiers is at least partially due to their lower body mass.

Adipose Tissue↗
PubMed1995

Linkage analysis of 19 candidate regions for insulin resistance in familial NIDDM.

As part of an ongoing search for diabetes susceptibility loci, we tested linkage with non-insulin-dependent diabetes mellitus (NIDDM) for 19 candidate loci or regions chosen for their potential to affect directly or indirectly the action of insulin. Loci were associated with insulin resistance, known effects on lipid metabolism, or effects on glucose metabolism or insulin action. Loci included the insulin-responsive (GLUT4) glucose transporter, hexokinase 2, glucagon, growth hormone, insulin receptor substrate 1 (IRS1), phosphoenolpyruvate carboxykinase, hepatic and muscle forms of pyruvate kinase, hepatic phosphofructokinase, the apolipoprotein B and the apolipoprotein A2 cluster, lipoprotein lipase, hepatic triglyceride lipase, the very-low-density-lipoprotein receptor, and the Pima insulin resistance locus on chromosome 4. For several candidates, no specific informative marker was available; consequently, we tested the surrounding region with highly informative markers. These regions included the diabetes-associated ras-like gene, rad, and the cholesterol ester-transfer gene, both mapped to chromosome 16. Additionally, we tested for linkage with markers at the tumor necrosis factor-alpha gene and the Friedreich's ataxia region. All regions were tested for linkage with microsatellite polymorphisms in > 450 individuals from a minimum of 16 Caucasian families under parametric (LINKAGE 5.1) and nonparametric (affected pedigree member) models.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1995

Disseminated acanthamebiasis in patients with AIDS. A report of five cases and a review of the literature.

BACKGROUND: Acanthamoeba and Leptomyxida are free-living amebae that cause granulomatous amebic encephalitis, a rare, slowly progressive, fatal neurologic process seen in immunosuppressed hosts. In addition, these organisms produce disseminated cutaneous lesions and involve other organs, particularly in patients with the acquired immunodeficiency syndrome (AIDS). RESULTS: We report five cases of disseminated acanthamebiasis in patients with AIDS, each with cutaneous manifestations but lacking central nervous system involvement. The medial CD4+ T-cell count was 0.024 x 10(9)/L. Skin lesions included pustules, subcutaneous and deep dermal nodules, and ulcers, most often seen on the extremities and face. Histopathologically, both pustular and vasculitic changes were observed; in all cases, the microscopic identification of organisms was difficult because of the macrophagelike appearance of the microbes in routine sections. CONCLUSIONS: Skin lesions are the most common reported presentation of infections caused by Acanthamoeba and Leptomyxida organisms in patients with AIDS, a minority of whom have central nervous system manifestations. A high index of suspicion is necessary for both the dermatologist and the dermatopathologist. Prognosis is guarded, but early treatment using a combination of intravenous pentamidine and oral fluconazole, sulfadiazine, and flucytosine may be beneficial.

Acanthamoeba↗
PubMed1995

Acantholytic epidermolysis bullosa.

BACKGROUND: We describe a new variant of inherited epidermolysis bullosa and elucidate the clinical, histologic, and ultrastructural features of this condition. OBSERVATIONS: This form of epidermolysis bullosa displays an autosomal dominant inheritance pattern, is characterized by acral bullae, and histologically demonstrates suprabasal clefting with acantholysis. Ultrastructural findings are nonspecific but reminiscent of those observed in benign familial pemphigus. CONCLUSION: Acantholytic epidermolysis bullosa is a rare but distinct clinicopathologic entity that warrants inclusion in the nosologic classification of epidermolysis bullosa.

Acantholysis↗
PubMed1995

Lactate response to uphill roller skiing: diagonal stride versus double pole techniques.

The purpose of this experiment was to investigate the lactate responses to roller skiing with double pole and diagonal stride techniques in eight collegiate or national level cross-country ski racers. Four-minute exercise stages were performed on a ski treadmill at 67, 94, 121, 148, and 174 m.min-1 on a 1.7% grade and at 67, 94, and 121 m.min-1 on a 7.1% grade. Whole blood lactate concentration, heart rate, oxygen consumption (VO2) and rating of perceived exertion (RPE) were determined at each exercise stage. Blood lactate concentrations were not significantly different between double poling and diagonal striding at the 1.7% grade. However, the blood lactate concentrations were higher during double poling at the 7.1% grade for all speeds. Blood lactate concentrations were also higher for double poling at the 7.1% grade when compared with diagonal striding at 70% of technique specific peak VO2, a heart rate of 145 and a RPE of 12. We conclude that blood lactate concentrations do not offer physiological justification for choosing one technique over the other when skiing on low grades, but low blood lactate concentrations may provide a physiological advantage for diagonal striding on steep grades.

Exercise↗
PubMed1994

Selective regulation of human neutrophil functions by the cell activation inhibitor CI-959.

The cell activation inhibitor CI-959 [5-methoxy-3-(1-methylethoxy)-N-1H-tetrazol-5-ylbenzo[ b]thiophene-2- carboxamide, monosodium salt] was evaluated for its effects on human neutrophil functions. CI-959 inhibited spontaneous migration and chemotaxis toward N-formyl-methionyl-L-leucyl-L-phenylalanine (fMLP) with 50% inhibition (IC50) values of 3.6 and 3.1 microM, respectively. CI-959 also inhibited superoxide anion generation in response to C5a, fMLP, serum-opsonized zymosan (SOZ), concanavalin A (Con A), and calcium ionophore A23187 with IC50 values of 2.5, 4.7, 14.5, 5.4, and 14.8 microM, respectively. In comparison, CI-959 inhibited myeloperoxidase microM, respectively. In comparison, CI-959 inhibited myeloperoxidase release in response to C5a, fMLP, SOZ, and Con A with IC50 values of 11.6, 16.1, 7.5, and < 1.0 microM, respectively, while inhibiting the response to A23187 by only 5.5% at 100 microM. At concentrations up to 100 microM, CI-959 had no effect on the respiratory burst or degranulation in response to L-alpha-1,2-dioctanoylglycerol (DiC8) or phorbol 12-myristate 13-acetate (PMA). In addition, the compound inhibited leukotriene B4 release stimulated by fMLP and SOZ (IC50 values 4.0 and 2.5 microM, respectively), while having less activity against the A23187-stimulated response (IC50 > 100 microM). These results demonstrate that CI-959 inhibits cellular responses to stimuli that mobilize intracellular calcium. For cellular responses to inophore-mediated calcium influx, only oxygen radical production was inhibited by CI-959. CI-959 was further evaluated for its effects on neutrophil stimulus-response coupling. At 100 microM, CI-959 had no effect on human neutrophil phospholipase C or protein kinase C. CI-959 inhibited fMLP-stimulated intracellular calcium mobilization and calcium influx with IC50 values of 16.7 and 3.1 microM, respectively, and exhibited less potent calmodulin antagonist activity (IC50 = 90.5 microM). These results indicate that CI-959 may exert its stimulus- and response-specific inhibitory effects on neutrophil functions, in part, through inhibition of calcium-regulated signalling mechanisms.

Arachidonic Acid↗
PubMed1994

Physiological comparison of uphill roller skiing: diagonal stride versus double pole.

The physiological responses to treadmill roller skiing with the double pole (DP) and diagonal stride (DS) techniques were compared at 1.7% and 7.1% grades among eight cross-country ski racers. Oxygen uptake (VO2) requirements were found to be lower (P < 0.05) for DP at the 1.7% grade, but similar at the 7.1% grade. In contrast, ratings of perceived exertion (RPE) and percentages of technique-specific peak VO2 were similar between techniques at the 1.7% grade, and lower (P < 0.05) for DS at the 7.1% grade. RPE and percentages of technique-specific peak VO2 were strongly correlated (r = 0.89). The primary findings indicate that 1) the economies for DP and DS are dependent upon the incline, 2) it is possible for the economy of DP to be greater than DS although the percentages of technique-specific peak VO2 are similar, and 3) the perceived effort associated with the use of DP and DS reflects the percentage of technique-specific peak VO2.

Adipose Tissue↗
PubMed1993

Exercise responses to in-line skating: comparisons to running and cycling.

A comparison of the physiological responses to in-line skating with the more traditional modes of exercise training has not been reported. The purpose of this study was to examine the physiological responses to in-line skating compared with running and cycling. Nine trained volunteers (2 male, 7 female) performed 3-6 submaximal (30-90% VO2max) workloads with each exercise mode. Oxygen uptake, heart rate and blood lactate were measured during each trial. Across the spectrum of oxygen uptakes studied, heart rate was higher with in-line skating than with cycling or running. At a lactate concentration of 4 mM, oxygen uptake was less for in-line skating and cycling than for running. Therefore, while in-line skating may be an effective mode of aerobic exercise, the training adaptations for in-line skating at 4 mM lactate may not be as great as for running, and at a given HR may be less than for running and cycling.

Adult↗
PubMed1993

Physiological responses to specific maximal exercise tests for cross-country skiing.

The present study attempted to quantify differences in peak physiological responses to pole-striding (PS), double poling on roller skis (DP), and diagonal striding on roller skis (DS) during maximal exercise. Six expert cross-country ski racers (3 M, 3 F) with a mean age of 20.2 +/- 1.3 yrs served as subjects. Testing was conducted on a motorized ski treadmill with a tracked belt surface. Expired air was analyzed continuously via an automated open-circuit system and averaged each 20 s. Heart rate was monitored via telemetry and arterialized blood was collected within 1 min of test termination and analyzed immediately for lactate. Peak values for heart rate and blood lactate did not differ among techniques. Peak oxygen uptake was higher for PS and DS versus DP whereas no difference was found between PS and DS. The VO2peak for DP was 77 and 81% of VO2peak for PS and DS, respectively. It was concluded that despite similar peak heart rate and blood lactate values, DP elicits a lower VO2peak than DS or PS and that PS responses appear to closely reflect those of DS.

Adult↗
PubMed1993

Physiologic responses to shoveling and thermal stress in men with cardiac disease.

To investigate the effect of temperature stress on responses to static-dynamic work in patients with ischemic heart disease (IHD), 10 men with IHD shoveled gravel for 30 min in a warm (29 degrees C), neutral (24 degrees C), and cold (-8 degrees C) environment (on separate days). A pace of 15 lifts.min-1 was set, and the load per lift approximated 5.5 kg. Heart rate (HR), oxygen consumption (VO2), and systolic (SBP) and diastolic blood pressures (DBP) were evaluated at 5-min intervals. Arrhythmias and ST-segment depression were evaluated by ambulatory electrocardiographic monitoring. At 30 min, VO2, SBP, and DBP were higher (P < 0.05) in the cold environment, and HR was higher (P < 0.05) in the warm environment compared with the neutral environment. HR increased (P < 0.05) from 5 to 30 min in all three conditions. The increase in HR was greater (P < 0.05) in the warm environment. None of the subjects reported angina or demonstrated electrocardiographic ST-segment changes during shoveling in any environment. The results indicate that low-risk patients with stable IHD show modest temperature-induced alterations in hemodynamic and VO2 responses during 30 min of moderate intensity (50-60% of peak VO2) static-dynamic work without adverse electrocardiographic responses or symptomatology.

Aged↗
PubMed1992

Effect of age and coronary artery disease on response to snow shoveling.

OBJECTIVES: The objective of this study was to evaluate the effect of age and coronary artery disease on responses to snow shoveling. BACKGROUND: Little information is available on the hemodynamic and metabolic responses to snow shoveling. METHODS: Sixteen men with asymptomatic coronary artery disease and relatively good functional work capacity, 13 older normal men and 12 younger normal men shoveled snow at a self-paced rate. Oxygen consumption, heart rate and blood pressure were determined. In nine men with coronary artery disease left ventricular ejection fraction was evaluated with an ambulatory radionuclide recorder. RESULTS: Oxygen consumption during snow shoveling differed (p < 0.05) among groups; it was lowest (18.5 +/- 0.8 ml/kg per min) in those with coronary artery disease, intermediate (22.2 +/- 0.9 ml/kg/min) in older normal men and highest (25.6 +/- 1.3 ml/kg/min) in younger normal men. Percent peak treadmill oxygen consumption and heart rate with shoveling in the three groups ranged from 60% to 68% and 75% to 78%, respectively. Left ventricular ejection fraction and frequency of arrhythmias during shoveling were similar to those during treadmill testing. CONCLUSIONS: During snow shoveling 1) the rate of energy expenditure selected varied in relation to each man's peak oxygen consumption; 2) older and younger normal men and asymptomatic men with coronary artery disease paced themselves at similar relative work intensities; 3) the work intensity selected represented hard work but was within commonly recommended criteria for aerobic exercise training; and 4) arrhythmias and left ventricular ejection fraction were similar to those associated with dynamic exercise.

Adult↗